6D6

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$40.00
SKU: 6D6
View product citations for antibody 6D6 on CiteAb

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Available: 100

DSHB Data Sheet

Catalog Fields

Antigen: Decorin
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Pringle, G.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Temple University
Positive Tested Species Reactivity: Bovine, Canine, Chick, Elk, Equine, Human, Mouse, Porcine, Rabbit
Depositors Notes: For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the 6D6 epitope.
Antigen Molecular Weight: 39.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DCN
Immunogen: Bovine skin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 315.43
Epitope Location or Sequence: C-terminal core protein a.a. 270-273
Uniprot ID: P21793 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 280760 
Additional Characterization:  
Antibody Registry ID: AB_1157861 
Additional Information: RRID:AB_1157861
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6D6 was deposited to the DSHB by Pringle, G.A. (DSHB Hybridoma Product 6D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

34 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Mapping the locations of the epitopes of five monoclonal antibodies to the core protein of dermatan sulfate proteoglycan II (decorin).
    Pringle GA
    The Journal of biological chemistry 268.16 (1993 Jun 5): 11558-64.

    Immunohistochemical localization of the proteoglycans decorin, biglycan and versican and transforming growth factor-beta in human post-burn hypertrophic and mature scars.
    Rahemtulla F
    Histopathology 26.5 (1995 May): 423-31.

    Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Strauss BH
    The American journal of pathology 163.3 (2003 Sep): 869-78.

    Age-related changes in the proteoglycans of human skin. Specific cleavage of decorin to yield a major catabolic fragment in adult skin.
    Caplan AI
    The Journal of biological chemistry 278.19 (2003 May 9): 17566-72.

    Light and X-ray scattering show decorin to be a dimer in solution.
    Bishop PN
    The Journal of biological chemistry 278.20 (2003 May 16): 18353-9.

    Increased diameters of collagen fibrils precipitated in vitro in the presence of decorin from various connective tissues.
    Scott PG
    Connective tissue research 36.4 (1997): 287-96.

    Isolation and characterization of proteoglycans from growing antlers of wapiti (Cervus elaphus).
    Sim JS
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 121.4 (1998 Dec): 437-42.

    Fibroblasts from post-burn hypertrophic scar tissue synthesize less decorin than normal dermal fibroblasts.
    Tredget EE
    Clinical science (London, England : 1979) 94.5 (1998 May): 541-7.

    Effect of decorin and dermatan sulfate on the mechanical properties of a neocartilage.
    Westendorf JJ
    Connective tissue research 51.2 (2010 Apr): 159-70.

    Vascular accumulation of the small leucine-rich proteoglycan decorin in CADASIL.
    Wang MM
    Neuroreport 25.13 (2014 Sep 10): 1059-63.

    Glycosaminoglycans and proteoglycans from different zones of the porcine knee meniscus.
    Scott PG
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 15.2 (1997 Mar): 213-20.

    Purification of decorin core protein from human lung tissue.
    Bischoff R
    Journal of chromatography. A 1123.2 (2006 Aug 11): 151-9.

    In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
    Strauss BH
    Cardiovascular research 65.3 (2005 Feb 15): 702-10.

    Effect of dermatan sulfate on the indentation and tensile properties of articular cartilage.
    Lewis JL
    Osteoarthritis and cartilage 17.5 (2009 May): 655-61.

    A comparison of in vivo gene delivery methods for antisense therapy in ligament healing.
    Frank CB
    Gene therapy 5.11 (1998 Nov): 1455-61.

    Murine tendon function is adversely affected by aggrecan accumulation due to the knockout of ADAMTS5.
    Plaas A
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 30.4 (2012 Apr): 620-6.

    Biochemical identification and immunolocalizaton of aggrecan, ADAMTS5 and inter-alpha-trypsin-inhibitor in equine degenerative suspensory ligament desmitis.
    Galante JO
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 29.6 (2011 Jun): 900-6.

    Extraction and characterization of the tissue forms of collagen types II and IX from bovine vitreous.
    Ayad S
    The Biochemical journal 299 ( Pt 2). (1994 Apr 15): 497-505.

    Ageing of equine articular cartilage: structure and composition of aggrecan and decorin.
    Bayliss MT
    Equine veterinary journal 30.1 (1998 Jan): 43-52.

    IHC References

    Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis.
    Borowiak M
    Nature communications 9.1 (2018 Aug 22): 3356.

    Decorin and biglycan retain LDL in disease-prone valvular and aortic subendothelial intimal matrix.
    Balaban RS
    Atherosclerosis 233.1 (2014 Mar): 113-21.

    Immunoelectron microscopic localization of the core protein of decorin near the d and e bands of tendon collagen fibrils by use of monoclonal antibodies.
    Dodd CM
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.10 (1990 Oct): 1405-11.

    Immunohistochemical localization of the proteoglycans decorin, biglycan and versican and transforming growth factor-beta in human post-burn hypertrophic and mature scars.
    Rahemtulla F
    Histopathology 26.5 (1995 May): 423-31.

    Localization of transforming growth factor beta and its natural inhibitor decorin in the human placenta and decidua throughout gestation.
    Lala PK
    Placenta 16.3 (1995 Apr): 221-31.

    An immunohistochemical study of the localization of biglycan, decorin and large chondroitin-sulphate proteoglycan in adult rat temporomandibular joint disc.
    Mitani H
    Archives of oral biology 43.11 (1998 Nov): 889-98.

    Age-related changes in the proteoglycans of human skin. Specific cleavage of decorin to yield a major catabolic fragment in adult skin.
    Caplan AI
    The Journal of biological chemistry 278.19 (2003 May 9): 17566-72.

    Light microscopic histochemical and immunohistochemical localisation of sulphated glycosaminoglycans in the rooster comb and wattle tissues.
    Sim JS
    Journal of anatomy 189 ( Pt 3). (1996 Dec): 643-50.

    Vascular accumulation of the small leucine-rich proteoglycan decorin in CADASIL.
    Wang MM
    Neuroreport 25.13 (2014 Sep 10): 1059-63.

    Decorin and biglycan retain LDL in disease-prone valvular and aortic subendothelial intimal matrix.
    Balaban RS
    Atherosclerosis 233.1 (2014 Mar): 113-21.

    Proteoglycan expression in the rat temporomandibular joint in response to unilateral bite raise.
    Scott PG
    Journal of dental research 77.7 (1998 Jul): 1520-8.

    Cellular and molecular pathology of HTS: basis for treatment.
    Tredget EE
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 15 Suppl 1. (2007 Sep-Oct): S6-17.

    Murine tendon function is adversely affected by aggrecan accumulation due to the knockout of ADAMTS5.
    Plaas A
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 30.4 (2012 Apr): 620-6.

    The formation of extracellular matrix during chondrogenic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I.
    Götting C
    Stem cells (Dayton, Ohio) 24.10 (2006 Oct): 2252-61.

    A method to cleave target molecules in a neocartilage.
    Lewis JL
    Connective tissue research 53.5 (2012): 430-6.

    Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine.
    Agrawal DK
    Arthritis research & therapy 18.1 (2016 Sep 13): 203.

    IP References
    ELISA References

    Mapping the locations of the epitopes of five monoclonal antibodies to the core protein of dermatan sulfate proteoglycan II (decorin).
    Pringle GA
    The Journal of biological chemistry 268.16 (1993 Jun 5): 11558-64.

    De novo synthesis of human dermis in vitro in the absence of a three-dimensional scaffold.
    Parenteau NL
    In vitro cellular & developmental biology. Animal 45.8 (2009 Sep): 430-41.

    FFPE References
    Epitope Map References
    All References

    Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis.
    Borowiak M
    Nature communications 9.1 (2018 Aug 22): 3356.

    Decorin and biglycan retain LDL in disease-prone valvular and aortic subendothelial intimal matrix.
    Balaban RS
    Atherosclerosis 233.1 (2014 Mar): 113-21.

    Immunoelectron microscopic localization of the core protein of decorin near the d and e bands of tendon collagen fibrils by use of monoclonal antibodies.
    Dodd CM
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.10 (1990 Oct): 1405-11.

    Immunohistochemical localization of the proteoglycans decorin, biglycan and versican and transforming growth factor-beta in human post-burn hypertrophic and mature scars.
    Rahemtulla F
    Histopathology 26.5 (1995 May): 423-31.

    Localization of transforming growth factor beta and its natural inhibitor decorin in the human placenta and decidua throughout gestation.
    Lala PK
    Placenta 16.3 (1995 Apr): 221-31.

    An immunohistochemical study of the localization of biglycan, decorin and large chondroitin-sulphate proteoglycan in adult rat temporomandibular joint disc.
    Mitani H
    Archives of oral biology 43.11 (1998 Nov): 889-98.

    Age-related changes in the proteoglycans of human skin. Specific cleavage of decorin to yield a major catabolic fragment in adult skin.
    Caplan AI
    The Journal of biological chemistry 278.19 (2003 May 9): 17566-72.

    Light microscopic histochemical and immunohistochemical localisation of sulphated glycosaminoglycans in the rooster comb and wattle tissues.
    Sim JS
    Journal of anatomy 189 ( Pt 3). (1996 Dec): 643-50.

    Vascular accumulation of the small leucine-rich proteoglycan decorin in CADASIL.
    Wang MM
    Neuroreport 25.13 (2014 Sep 10): 1059-63.

    Proteoglycan expression in the rat temporomandibular joint in response to unilateral bite raise.
    Scott PG
    Journal of dental research 77.7 (1998 Jul): 1520-8.

    Cellular and molecular pathology of HTS: basis for treatment.
    Tredget EE
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 15 Suppl 1. (2007 Sep-Oct): S6-17.

    Murine tendon function is adversely affected by aggrecan accumulation due to the knockout of ADAMTS5.
    Plaas A
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 30.4 (2012 Apr): 620-6.

    The formation of extracellular matrix during chondrogenic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I.
    Götting C
    Stem cells (Dayton, Ohio) 24.10 (2006 Oct): 2252-61.

    A method to cleave target molecules in a neocartilage.
    Lewis JL
    Connective tissue research 53.5 (2012): 430-6.

    Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine.
    Agrawal DK
    Arthritis research & therapy 18.1 (2016 Sep 13): 203.

    A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the <i>Drosophila</i> motor system.
    Yao CK
    eLife 8. (2019 Jul 18): .

    Mapping the locations of the epitopes of five monoclonal antibodies to the core protein of dermatan sulfate proteoglycan II (decorin).
    Pringle GA
    The Journal of biological chemistry 268.16 (1993 Jun 5): 11558-64.

    Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Strauss BH
    The American journal of pathology 163.3 (2003 Sep): 869-78.

    Light and X-ray scattering show decorin to be a dimer in solution.
    Bishop PN
    The Journal of biological chemistry 278.20 (2003 May 16): 18353-9.

    Increased diameters of collagen fibrils precipitated in vitro in the presence of decorin from various connective tissues.
    Scott PG
    Connective tissue research 36.4 (1997): 287-96.

    Isolation and characterization of proteoglycans from growing antlers of wapiti (Cervus elaphus).
    Sim JS
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 121.4 (1998 Dec): 437-42.

    Fibroblasts from post-burn hypertrophic scar tissue synthesize less decorin than normal dermal fibroblasts.
    Tredget EE
    Clinical science (London, England : 1979) 94.5 (1998 May): 541-7.

    Effect of decorin and dermatan sulfate on the mechanical properties of a neocartilage.
    Westendorf JJ
    Connective tissue research 51.2 (2010 Apr): 159-70.

    Glycosaminoglycans and proteoglycans from different zones of the porcine knee meniscus.
    Scott PG
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 15.2 (1997 Mar): 213-20.

    Purification of decorin core protein from human lung tissue.
    Bischoff R
    Journal of chromatography. A 1123.2 (2006 Aug 11): 151-9.

    In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
    Strauss BH
    Cardiovascular research 65.3 (2005 Feb 15): 702-10.

    Effect of dermatan sulfate on the indentation and tensile properties of articular cartilage.
    Lewis JL
    Osteoarthritis and cartilage 17.5 (2009 May): 655-61.

    A comparison of in vivo gene delivery methods for antisense therapy in ligament healing.
    Frank CB
    Gene therapy 5.11 (1998 Nov): 1455-61.

    Biochemical identification and immunolocalizaton of aggrecan, ADAMTS5 and inter-alpha-trypsin-inhibitor in equine degenerative suspensory ligament desmitis.
    Galante JO
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 29.6 (2011 Jun): 900-6.

    Extraction and characterization of the tissue forms of collagen types II and IX from bovine vitreous.
    Ayad S
    The Biochemical journal 299 ( Pt 2). (1994 Apr 15): 497-505.

    Ageing of equine articular cartilage: structure and composition of aggrecan and decorin.
    Bayliss MT
    Equine veterinary journal 30.1 (1998 Jan): 43-52.

    TGF beta 1 and biglycan, decorin, and fibromodulin metabolism in canine cartilage.
    Cs-Szabó G
    Osteoarthritis and cartilage 11.3 (2003 Mar): 167-76.

    De novo synthesis of human dermis in vitro in the absence of a three-dimensional scaffold.
    Parenteau NL
    In vitro cellular & developmental biology. Animal 45.8 (2009 Sep): 430-41.

    Production and characterization of monoclonal antibodies to bovine skin proteodermatan sulfate.
    Mosmann TR
    Collagen and related research 5.1 (1985 Jan): 23-39.

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