6D6

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$40.00
SKU: 6D6
View product citations for antibody 6D6 on CiteAb

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Available: 115

DSHB Data Sheet

Catalog Fields

Antigen: Decorin
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Pringle, G.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Temple University
Positive Tested Species Reactivity: Bovine, Canine, Chick, Elk, Equine, Human, Mouse, Porcine, Rabbit
Depositors Notes: For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the 6D6 epitope.
Antigen Molecular Weight: 39.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DCN
Immunogen: Bovine skin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 315.43
Epitope Location or Sequence: C-terminal core protein a.a. 270-273
Uniprot ID: P21793 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 280760 
Additional Characterization:
Antibody Registry ID: AB_1157861 
Additional Information: RRID:AB_1157861
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6D6 was deposited to the DSHB by Pringle, G.A. (DSHB Hybridoma Product 6D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

34 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References
    FFPE References
    Epitope Map References
    All References

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