Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Pringle, G.A.
Host Species: mouse
Depositors Institution: Temple University
Positive Tested Species Reactivity: Bovine, Canine, Chick, Elk, Equine, Human, Mouse, Porcine, Rabbit
Depositors Notes: For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the 6D6 epitope.
Antigen Molecular Weight: 39.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Bovine skin
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 315.43
Epitope Location or Sequence: C-terminal core protein a.a. 270-273
Uniprot ID: P21793
Immunogen Sequence: Full length sequence
Entrez Gene ID: 280760
Antibody Registry ID: AB_1157861
Additional Information: RRID:AB_1157861
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6D6 was deposited to the DSHB by Pringle, G.A. (DSHB Hybridoma Product 6D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References