M3F7

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$40.00
SKU: M3F7
View product citations for antibody M3F7 on CiteAb

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Available: 265

DSHB Data Sheet

Catalog Fields

Antigen: Collagen, type IV, alpha 1/ collagen, type IV alpha 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Furthmayr, H.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Human, Quail, Rat
Depositors Notes: M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.
Antigen Molecular Weight: 100 kDa/ 50 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: COL4A1/COL4A2
Immunogen: Human placenta
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: Triple helical domain about 900 Å from the N-terminus
Uniprot ID: P02462 
Immunogen Sequence: alpha1 (IV)-alpha2(IV) triple helix
Entrez Gene ID: 1282 
Additional Characterization:
Antibody Registry ID: AB_528167 
Additional Information: RRID:AB_528167
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M3F7 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product M3F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

30 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    IF References

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms.
    Kenney MC
    Laboratory investigation; a journal of technical methods and pathology 72.4 (1995 Apr): 461-73.

    Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane.
    Shimizu H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.1 (2006 Jan): 109-18.

    Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures.
    Deschaseaux F
    Scientific reports 9.1 (2019 May 10): 7250.

    Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.
    Lansford R
    Frontiers in cell and developmental biology 7. (2019): 35.

    Novel Function of Serine Protease HTRA1 in Inhibiting Adipogenic Differentiation of Human Mesenchymal Stem Cells via MAP Kinase-Mediated MMP Upregulation.
    Richards PJ
    Stem cells (Dayton, Ohio) 34.6 (2016 Jun): 1601-14.

    Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo.
    Müller HW
    PloS one 10.7 (2015): e0134371.

    Preferential utilization of perilipin 2-associated intramuscular triglycerides during 1 h of moderate-intensity endurance-type exercise.
    Shaw CS
    Experimental physiology 97.8 (2012 Aug): 970-80.

    Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation.
    Konttinen YT
    Journal of cellular and molecular medicine 16.7 (2012 Jul): 1485-95.

    Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
    Beaulieu JF
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 18.1 (2010 Jan-Feb): 114-22.

    Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines.
    Binas B
    PloS one 4.9 (2009 Sep 28): e7216.

    Cardiac cushions modulate action potential phenotype during heart development [corrected].
    Modgi A
    Developmental dynamics : an official publication of the American Association of Anatomists 238.3 (2009 Mar): 611-23.

    Blood vessels of human islets of Langerhans are surrounded by a double basement membrane.
    Otonkoski T
    Diabetologia 51.7 (2008 Jul): 1181-91.

    Reperfusion activates metalloproteinases that contribute to neurovascular injury.
    Sharp FR
    Experimental neurology 210.2 (2008 Apr): 549-59.

    Mutations of ESRRB encoding estrogen-related receptor beta cause autosomal-recessive nonsyndromic hearing impairment DFNB35.
    Kremer H
    American journal of human genetics 82.1 (2008 Jan): 125-38.

    Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor.
    Lin HY
    Diabetes 56.2 (2007 Feb): 380-8.

    WB References
    IHC References

    Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue.
    Bürgi U
    Thyroid : official journal of the American Thyroid Association 7.3 (1997 Jun): 347-56.

    Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents.
    Malda J
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 15.2 (2007 Mar-Apr): 266-74.

    Human skeletal muscle xenograft as a new preclinical model for muscle disorders.
    Wagner KR
    Human molecular genetics 23.12 (2014 Jun 15): 3180-8.

    No changes in cerebellar microvessel length density in sudden infant death syndrome: implications for pathogenetic mechanisms.
    Schmitz C
    Journal of neuropathology and experimental neurology 73.4 (2014 Apr): 312-23.

    A rapid in vivo assay system for analyzing the organogenetic capacity of human kidney cells.
    Goldstein RS
    Organogenesis 7.2 (2011 Apr-Jun): 140-4.

    Peroxynitrite decomposition catalyst prevents matrix metalloproteinase activation and neurovascular injury after prolonged cerebral ischemia in rats.
    Lu A
    Journal of neurochemistry 115.5 (2010 Dec): 1266-76.

    Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
    Bassuk JA
    American journal of physiology. Renal physiology 299.5 (2010 Nov): F1094-110.

    The treatment of traumatic brain injury with velcade.
    Chopp M
    Journal of neurotrauma 27.9 (2010 Sep): 1625-34.

    Abnormal basement membrane type IV collagen alpha-chain composition in labial salivary glands in Sjögren's syndrome.
    Konttinen YT
    Arthritis and rheumatism 60.4 (2009 Apr): 938-45.

    Compositional differences between infant and adult human corneal basement membranes.
    Ljubimov AV
    Investigative ophthalmology and visual science 48.11 (2007 Nov): 4989-99.

    IP References

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    ELISA References

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    FFPE References
    Epitope Map References

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    All References

    Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue.
    Bürgi U
    Thyroid : official journal of the American Thyroid Association 7.3 (1997 Jun): 347-56.

    Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents.
    Malda J
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 15.2 (2007 Mar-Apr): 266-74.

    Human skeletal muscle xenograft as a new preclinical model for muscle disorders.
    Wagner KR
    Human molecular genetics 23.12 (2014 Jun 15): 3180-8.

    No changes in cerebellar microvessel length density in sudden infant death syndrome: implications for pathogenetic mechanisms.
    Schmitz C
    Journal of neuropathology and experimental neurology 73.4 (2014 Apr): 312-23.

    A rapid in vivo assay system for analyzing the organogenetic capacity of human kidney cells.
    Goldstein RS
    Organogenesis 7.2 (2011 Apr-Jun): 140-4.

    Peroxynitrite decomposition catalyst prevents matrix metalloproteinase activation and neurovascular injury after prolonged cerebral ischemia in rats.
    Lu A
    Journal of neurochemistry 115.5 (2010 Dec): 1266-76.

    Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
    Bassuk JA
    American journal of physiology. Renal physiology 299.5 (2010 Nov): F1094-110.

    The treatment of traumatic brain injury with velcade.
    Chopp M
    Journal of neurotrauma 27.9 (2010 Sep): 1625-34.

    Abnormal basement membrane type IV collagen alpha-chain composition in labial salivary glands in Sjögren's syndrome.
    Konttinen YT
    Arthritis and rheumatism 60.4 (2009 Apr): 938-45.

    Compositional differences between infant and adult human corneal basement membranes.
    Ljubimov AV
    Investigative ophthalmology and visual science 48.11 (2007 Nov): 4989-99.

    Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Furthmayr H
    Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.

    Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms.
    Kenney MC
    Laboratory investigation; a journal of technical methods and pathology 72.4 (1995 Apr): 461-73.

    Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane.
    Shimizu H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.1 (2006 Jan): 109-18.

    Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures.
    Deschaseaux F
    Scientific reports 9.1 (2019 May 10): 7250.

    Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.
    Lansford R
    Frontiers in cell and developmental biology 7. (2019): 35.

    Novel Function of Serine Protease HTRA1 in Inhibiting Adipogenic Differentiation of Human Mesenchymal Stem Cells via MAP Kinase-Mediated MMP Upregulation.
    Richards PJ
    Stem cells (Dayton, Ohio) 34.6 (2016 Jun): 1601-14.

    Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo.
    Müller HW
    PloS one 10.7 (2015): e0134371.

    Preferential utilization of perilipin 2-associated intramuscular triglycerides during 1 h of moderate-intensity endurance-type exercise.
    Shaw CS
    Experimental physiology 97.8 (2012 Aug): 970-80.

    Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation.
    Konttinen YT
    Journal of cellular and molecular medicine 16.7 (2012 Jul): 1485-95.

    Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
    Beaulieu JF
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 18.1 (2010 Jan-Feb): 114-22.

    Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines.
    Binas B
    PloS one 4.9 (2009 Sep 28): e7216.

    Cardiac cushions modulate action potential phenotype during heart development [corrected].
    Modgi A
    Developmental dynamics : an official publication of the American Association of Anatomists 238.3 (2009 Mar): 611-23.

    Blood vessels of human islets of Langerhans are surrounded by a double basement membrane.
    Otonkoski T
    Diabetologia 51.7 (2008 Jul): 1181-91.

    Reperfusion activates metalloproteinases that contribute to neurovascular injury.
    Sharp FR
    Experimental neurology 210.2 (2008 Apr): 549-59.

    Mutations of ESRRB encoding estrogen-related receptor beta cause autosomal-recessive nonsyndromic hearing impairment DFNB35.
    Kremer H
    American journal of human genetics 82.1 (2008 Jan): 125-38.

    Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor.
    Lin HY
    Diabetes 56.2 (2007 Feb): 380-8.

    Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.
    Keller KE
    Investigative ophthalmology and visual science 60.2 (2019 Feb 1): 843-851.

    Type IV collagen is a novel DEJ biomarker that is reduced by radiotherapy.
    Walker MP
    Journal of dental research 93.10 (2014 Oct): 1028-34.

    Enamel organic matrix: potential structural role in enamel and relationship to residual basement membrane constituents at the dentin enamel junction.
    Gorski JP
    Connective tissue research 55 Suppl 1. (2014 Aug): 33-7.

    Matrix metalloproteinase-2 deletions protect against hemorrhagic transformation after 1 h of cerebral ischemia and 23 h of reperfusion.
    Clark JF
    Neuroscience 253. (2013 Dec 3): 361-7.

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