(0)No Reviews yet
$45.00 to $425.00
SKU: AGP78.20
View product citations for antibody AGP78.20 on CiteAb

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Product Name/ID: AGP78.20
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Gp210
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528271 
Uniprot ID: Q9GPI0 
Entrez Gene ID: 35512 
Clonality: Monoclonal
Immunogen: Purified native NMPCL glycoprotein
Immunogen Sequence: Full length protein
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: No
Antigen Name: Integral membrane pore glycoprotein gp210
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/11/2003
Antigen Molecular Weight: 209 kDa
Depositor: Fisher, P.A.
Antigen Sequence:
Depositor Institution: SUNY at Stony Brook, Pharmacological Sciences
Antigen Species: Drosophila
Depositor Notes: This antibody recognizes a a glycoprotein component of the nuclear matrix-pore complex-lamina (NMPCL).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_528271. FBgn0266580.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AGP78.20 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product AGP78.20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review