N2 7A1 Armadillo
Catalog Fields
Antigen: Armadillo segment polarity protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a, kappa light chain
Depositor: Wieschaus, E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Institution: Princeton University
Antigen Molecular Weight: 110 kDa
Depositors Notes: This antigen is also known as armadillo drosophila protein. Armadillo is the drosophila homolog of mammalian beta-catenin.
Immunogen: Recombinant ATH-fusion protein
Gene: arm
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P18824
Epitope Location or Sequence:
Entrez Gene ID: 31151
Immunogen Sequence: a.a. 68-122 ( bp 2011-2175)
Antibody Registry ID: AB_528089
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: FLYBASE:FBgn0000117
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2 7A1 Armadillo was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product N2 7A1 Armadillo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
41 References
Initial Publication
Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
Wieschaus E
Cell 63.3 (1990 Nov 2): 549-60.