N2 7A1 Armadillo
Catalog Fields
Antigen: Armadillo segment polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Wieschaus, E.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Notes: This antigen is also known as armadillo drosophila protein. Armadillo is the drosophila homolog of mammalian beta-catenin.
Predicted Species Reactivity:
Gene: arm
Immunogen: Recombinant ATH-fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P18824
Immunogen Sequence: a.a. 68-122 ( bp 2011-2175)
Entrez Gene ID: 31151
Antibody Registry ID: AB_528089
Additional Information: FLYBASE:FBgn0000117
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2 7A1 Armadillo was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product N2 7A1 Armadillo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
41 References
Initial Publication
Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
Wieschaus E
Cell 63.3 (1990 Nov 2): 549-60.