N2 7A1 Armadillo
Catalog Fields
Antigen: Armadillo segment polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Wieschaus, E.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Notes: This antigen is also known as armadillo drosophila protein. Armadillo is the drosophila homolog of mammalian beta-catenin.
Antigen Molecular Weight: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: arm
Immunogen: Recombinant ATH-fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P18824
Immunogen Sequence: a.a. 68-122 ( bp 2011-2175)
Entrez Gene ID: 31151
Additional Characterization:
Antibody Registry ID: AB_528089
Additional Information: FLYBASE:FBgn0000117
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2 7A1 Armadillo was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product N2 7A1 Armadillo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
28 References
Initial Publication