N2 7A1 Armadillo

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$40.00
SKU: N2 7A1 Armadillo
View product citations for antibody N2 7A1 Armadillo on CiteAb

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DSHB Data Sheet
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Catalog Fields

Antigen: Armadillo segment polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Wieschaus, E.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Notes: This antigen is also known as armadillo drosophila protein. Armadillo is the drosophila homolog of mammalian beta-catenin.
Antigen Molecular Weight: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: arm
Immunogen: Recombinant ATH-fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P18824 
Immunogen Sequence: a.a. 68-122 ( bp 2011-2175)
Entrez Gene ID: 31151 
Additional Characterization:
Antibody Registry ID: AB_528089 
Additional Information: FLYBASE:FBgn0000117
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
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These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2 7A1 Armadillo was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product N2 7A1 Armadillo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

28 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
    • Initial Publication

    Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
    Wieschaus E
    Cell 63.3 (1990 Nov 2): 549-60.

    IF References

    A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche.
    Riechmann V
    Journal of cell science 127.Pt 12 (2014 Jun 15): 2736-48.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Socs36E attenuates STAT signaling to optimize motile cell specification in the Drosophila ovary.
    Starz-Gaiano M
    Developmental biology 379.2 (2013 Jul 15): 152-66.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity.
    Uemura T
    Developmental cell 19.3 (2010 Sep 14): 389-401.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Adult Muscle Formation Requires <i>Drosophila</i> Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors.
    Geisbrecht ER
    Genetics 206.1 (2017 May): 199-213.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Cell shape change and invagination of the cephalic furrow involves reorganization of F-actin.
    Thomas JH
    Developmental biology 402.2 (2015 Jun 15): 192-207.

    src64 and tec29 are required for microfilament contraction during Drosophila cellularization.
    Wieschaus E
    Development (Cambridge, England) 131.4 (2004 Feb): 863-71.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    JAK/STAT signaling is involved in air sac primordium development of Drosophila melanogaster.
    Srivastava A
    FEBS letters 593.7 (2019 Apr): 658-669.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly.
    Tepass U
    The Journal of cell biology 218.10 (2019 Oct 7): 3397-3414.

    WB References

    E-cadherin missense mutations, associated with hereditary diffuse gastric cancer (HDGC) syndrome, display distinct invasive behaviors and genetic interactions with the Wnt and Notch pathways in Drosophila epithelia.
    Casares F
    Human molecular genetics 15.10 (2006 May 15): 1704-12.

    Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
    Wieschaus E
    Cell 63.3 (1990 Nov 2): 549-60.

    A new secreted protein that binds to Wnt proteins and inhibits their activities.
    Nathans J
    Nature 398.6726 (1999 Apr 1): 431-6.

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    IHC References

    Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
    Barber DL
    eLife 4. (2015 Mar 20): .

    Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
    Wieschaus E
    Cell 63.3 (1990 Nov 2): 549-60.

    CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.
    Giangrande A
    Developmental biology 359.1 (2011 Nov 1): 37-46.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    IP References

    The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction.
    Peifer M
    Journal of cell science 105 ( Pt 4). (1993 Aug): 993-1000.

    Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    Epitope Map References

    Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
    Wieschaus E
    Cell 63.3 (1990 Nov 2): 549-60.

    All References

    Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
    Barber DL
    eLife 4. (2015 Mar 20): .

    Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless.
    Wieschaus E
    Cell 63.3 (1990 Nov 2): 549-60.

    CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.
    Giangrande A
    Developmental biology 359.1 (2011 Nov 1): 37-46.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche.
    Riechmann V
    Journal of cell science 127.Pt 12 (2014 Jun 15): 2736-48.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Socs36E attenuates STAT signaling to optimize motile cell specification in the Drosophila ovary.
    Starz-Gaiano M
    Developmental biology 379.2 (2013 Jul 15): 152-66.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity.
    Uemura T
    Developmental cell 19.3 (2010 Sep 14): 389-401.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    Adult Muscle Formation Requires <i>Drosophila</i> Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors.
    Geisbrecht ER
    Genetics 206.1 (2017 May): 199-213.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Cell shape change and invagination of the cephalic furrow involves reorganization of F-actin.
    Thomas JH
    Developmental biology 402.2 (2015 Jun 15): 192-207.

    src64 and tec29 are required for microfilament contraction during Drosophila cellularization.
    Wieschaus E
    Development (Cambridge, England) 131.4 (2004 Feb): 863-71.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    JAK/STAT signaling is involved in air sac primordium development of Drosophila melanogaster.
    Srivastava A
    FEBS letters 593.7 (2019 Apr): 658-669.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly.
    Tepass U
    The Journal of cell biology 218.10 (2019 Oct 7): 3397-3414.

    E-cadherin missense mutations, associated with hereditary diffuse gastric cancer (HDGC) syndrome, display distinct invasive behaviors and genetic interactions with the Wnt and Notch pathways in Drosophila epithelia.
    Casares F
    Human molecular genetics 15.10 (2006 May 15): 1704-12.

    A new secreted protein that binds to Wnt proteins and inhibits their activities.
    Nathans J
    Nature 398.6726 (1999 Apr 1): 431-6.

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction.
    Peifer M
    Journal of cell science 105 ( Pt 4). (1993 Aug): 993-1000.

    Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

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