Product Name/ID: BEN
Available to For-Profits: Yes
Alternate Antibody Name: hematopoetic/neuronal cell surface glycoprotein
Ab Isotype: MIgG1
Antibody Registry ID: AB_2313998
Immunogen: bursa of Fabricius
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: neuronal, motor, marker (SC-1)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/13/1992
Antigen Molecular Weight: 95-110 kDa
Depositor: Le Douarin, N. / Pourquie, O.
Depositor Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France
Antigen Species: chicken
Depositor Notes: Cell binding note: peripheral projecting neurons, climbing fibers, hemopoietic precursors, immature and activated T lymphocytes. IP note: immunoglobulin superfamily molecule identical to SC1 and DM-GRASP.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick, Quail
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BEN was deposited to the DSHB by Le Douarin, N. / Pourquie, O. (DSHB Hybridoma Product BEN)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).