39.4D5

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$40.00
SKU: 39.4D5
View product citations for antibody 39.4D5 on CiteAb

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Available: 398

DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 & Islet-2 homeobox
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish
Depositors Notes: MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006) TEST
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Isl1 & Isl2
Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)
Alternate Gene Names: ISL1, ISL LIM homeobox 1, ISL2; ISL LIM homeobox 2
Alternate Antibody Name: anti-islet1; anti-isl1;anti-islet2; anti-isl2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 178-349 of Isl1 protein
Uniprot ID: P61374 P50480 
Immunogen Sequence: Partial protein
Entrez Gene ID: 64444 57233 
Additional Characterization:
Antibody Registry ID: AB_2314683 
Additional Information: also detects Islet-2. The protein region used for immunization lacks the LIM motifs shared with other proteins.
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.4D5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.4D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

52 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Cell
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    IF References

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Neuron
    Castellani V
    Neuron 95.4 (2017 Aug 16): 834-851.e5.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Suter U
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.17 (2014 Apr 23): 6112-22.

    Glia
    Barresi MJ
    Glia 64.7 (2016 Jul): 1170-89.

    Genesis (New York, N.Y. : 2000)
    Gan L
    Genesis (New York, N.Y. : 2000) 57.10 (2019 Oct): e23328.

    eLife
    Chen JA
    eLife 7. (2018 Oct 12): .

    WB References

    The Journal of biological chemistry
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    IHC References

    Investigative ophthalmology & visual science
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    Developmental biology
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Cell
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Cell
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Developmental biology
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Journal of neuroscience research
    deLapeyrière O
    Journal of neuroscience research 55.1 (1999 Jan 1): 119-26.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Developmental biology
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    The Journal of comparative neurology
    Kaprielian Z
    The Journal of comparative neurology 439.3 (2001 Oct 22): 368-83.

    Genes & development
    Takada S
    Genes & development 16.5 (2002 Mar 1): 548-53.

    Current biology : CB
    Nabeshima Y
    Current biology : CB 12.13 (2002 Jul 9): 1157-63.

    The Journal of comparative neurology
    Gahr M
    The Journal of comparative neurology 453.1 (2002 Nov 4): 57-70.

    Biotechnology progress
    Hickman JJ
    Biotechnology progress 19.6 (2003 Nov-Dec): 1756-61.

    Investigative ophthalmology & visual science
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    Development (Cambridge, England)
    Johnson JE
    Development (Cambridge, England) 131.6 (2004 Mar): 1319-30.

    The Journal of clinical investigation
    Jessop M
    The Journal of clinical investigation 59.4 (1977 Apr): 652-8.

    Proceedings of the National Academy of Sciences of the United States of America
    Sasai Y
    Proceedings of the National Academy of Sciences of the United States of America 99.3 (2002 Feb 5): 1580-5.

    Neuroscience
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Proceedings of the National Academy of Sciences of the United States of America
    McLennan IS
    Proceedings of the National Academy of Sciences of the United States of America 102.45 (2005 Nov 8): 16421-5.

    Development (Cambridge, England)
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Developmental biology
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Gene expression patterns : GEP
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Nature
    Chien KR
    Nature 460.7251 (2009 Jul 2): 113-7.

    Developmental biology
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Development (Cambridge, England)
    Takebayashi H
    Development (Cambridge, England) 139.6 (2012 Mar): 1125-32.

    Cell
    Pfaff SL
    Cell 110.2 (2002 Jul 26): 237-49.

    Cell
    Jessell TM
    Cell 76.4 (1994 Feb 25): 761-75.

    Mechanisms of development
    Goldstone K
    Mechanisms of development 91.1-2 (2000 Mar 1): 69-80.

    Mechanisms of development
    Salbaum JM
    Mechanisms of development 71.1-2 (1998 Feb): 201-4.

    Developmental dynamics : an official publication of the American Association of Anatomists
    El-Hodiri HM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.2 (2005 Feb): 313-24.

    Development (Cambridge, England)
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Neuroscience
    Liu FC
    Neuroscience 103.4 (2001): 999-1016.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Katz LC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.44 (2004 Nov 3): 9962-70.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Appel B
    Developmental dynamics : an official publication of the American Association of Anatomists 241.2 (2012 Feb): 315-26.

    PloS one
    Bakkers J
    PloS one 7.10 (2012): e47644.

    Neural development
    Appel B
    Neural development 7. (2012 May 3): 15.

    Developmental biology
    Artinger KB
    Developmental biology 356.2 (2011 Aug 15): 496-505.

    Development (Cambridge, England)
    le Noble F
    Development (Cambridge, England) 138.10 (2011 May): 1935-45.

    Investigative ophthalmology & visual science
    Gross JM
    Investigative ophthalmology & visual science 52.12 (2011 Nov 17): 8868-77.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.9 (2010 Mar 3): 3254-63.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Proceedings of the National Academy of Sciences of the United States of America
    Stainier DY
    Proceedings of the National Academy of Sciences of the United States of America 107.3 (2010 Jan 19): 1142-7.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Eisen JS
    Developmental dynamics : an official publication of the American Association of Anatomists 238.12 (2009 Dec): 3226-36.

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Biology open
    Knabe W
    Biology open 7.6 (2018 Jun 19): .

    FFPE References

    Biology open
    Knabe W
    Biology open 7.6 (2018 Jun 19): .

    Epitope Map References

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Gel Supershift References

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    All References

    Investigative ophthalmology & visual science
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    Developmental biology
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Cell
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Cell
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Developmental biology
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Journal of neuroscience research
    deLapeyrière O
    Journal of neuroscience research 55.1 (1999 Jan 1): 119-26.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Developmental biology
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    The Journal of comparative neurology
    Kaprielian Z
    The Journal of comparative neurology 439.3 (2001 Oct 22): 368-83.

    Genes & development
    Takada S
    Genes & development 16.5 (2002 Mar 1): 548-53.

    Current biology : CB
    Nabeshima Y
    Current biology : CB 12.13 (2002 Jul 9): 1157-63.

    The Journal of comparative neurology
    Gahr M
    The Journal of comparative neurology 453.1 (2002 Nov 4): 57-70.

    Biotechnology progress
    Hickman JJ
    Biotechnology progress 19.6 (2003 Nov-Dec): 1756-61.

    Development (Cambridge, England)
    Johnson JE
    Development (Cambridge, England) 131.6 (2004 Mar): 1319-30.

    The Journal of clinical investigation
    Jessop M
    The Journal of clinical investigation 59.4 (1977 Apr): 652-8.

    Proceedings of the National Academy of Sciences of the United States of America
    Sasai Y
    Proceedings of the National Academy of Sciences of the United States of America 99.3 (2002 Feb 5): 1580-5.

    Neuroscience
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Proceedings of the National Academy of Sciences of the United States of America
    McLennan IS
    Proceedings of the National Academy of Sciences of the United States of America 102.45 (2005 Nov 8): 16421-5.

    Development (Cambridge, England)
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Gene expression patterns : GEP
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Nature
    Chien KR
    Nature 460.7251 (2009 Jul 2): 113-7.

    Developmental biology
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Development (Cambridge, England)
    Takebayashi H
    Development (Cambridge, England) 139.6 (2012 Mar): 1125-32.

    Cell
    Pfaff SL
    Cell 110.2 (2002 Jul 26): 237-49.

    Cell
    Jessell TM
    Cell 76.4 (1994 Feb 25): 761-75.

    Mechanisms of development
    Goldstone K
    Mechanisms of development 91.1-2 (2000 Mar 1): 69-80.

    Mechanisms of development
    Salbaum JM
    Mechanisms of development 71.1-2 (1998 Feb): 201-4.

    Developmental dynamics : an official publication of the American Association of Anatomists
    El-Hodiri HM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.2 (2005 Feb): 313-24.

    Development (Cambridge, England)
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Neuroscience
    Liu FC
    Neuroscience 103.4 (2001): 999-1016.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Katz LC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.44 (2004 Nov 3): 9962-70.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Appel B
    Developmental dynamics : an official publication of the American Association of Anatomists 241.2 (2012 Feb): 315-26.

    PloS one
    Bakkers J
    PloS one 7.10 (2012): e47644.

    Neural development
    Appel B
    Neural development 7. (2012 May 3): 15.

    Developmental biology
    Artinger KB
    Developmental biology 356.2 (2011 Aug 15): 496-505.

    Development (Cambridge, England)
    le Noble F
    Development (Cambridge, England) 138.10 (2011 May): 1935-45.

    Investigative ophthalmology & visual science
    Gross JM
    Investigative ophthalmology & visual science 52.12 (2011 Nov 17): 8868-77.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.9 (2010 Mar 3): 3254-63.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Proceedings of the National Academy of Sciences of the United States of America
    Stainier DY
    Proceedings of the National Academy of Sciences of the United States of America 107.3 (2010 Jan 19): 1142-7.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Eisen JS
    Developmental dynamics : an official publication of the American Association of Anatomists 238.12 (2009 Dec): 3226-36.

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Biology open
    Knabe W
    Biology open 7.6 (2018 Jun 19): .

    Neuron
    Castellani V
    Neuron 95.4 (2017 Aug 16): 834-851.e5.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Suter U
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.17 (2014 Apr 23): 6112-22.

    Glia
    Barresi MJ
    Glia 64.7 (2016 Jul): 1170-89.

    Genesis (New York, N.Y. : 2000)
    Gan L
    Genesis (New York, N.Y. : 2000) 57.10 (2019 Oct): e23328.

    eLife
    Chen JA
    eLife 7. (2018 Oct 12): .

    The Journal of biological chemistry
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

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