Clone ID / Name: 39.4D5

Anti - Islet-1 & Islet-2 homeobox

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TRKC expression in E11.5 ISL1+ DRG neurons using DSHB antibody 39.4D5 anti-Islet-1 & Islet-2 homeobox (blue). PMID: 31515492, Fig 1E.

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SKU: 39.4D5

Product Form :

Supernatant 1.0 ml

~ 25 – 43 μg/mL

Concentrate 0.1 ml

~ 277 – 372 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Available: 1

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Catalog Fields

Identity

Clone ID / Name: 39.4D5

Alternate Antibody Name: anti-islet1; anti-isl1;anti-islet2; anti-isl2

Antigen Name: Islet-1 & Islet-2 homeobox

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: Isl1 & Isl2

Entrez Gene ID: 64444, 57233

Antigen Sequence: —

Antigen Species: Rat

Antigen Mol. Weight: Predicted: 39kDa; Apparent: 39kDa

Uniprot ID: P61374, P50480

Human Protein Atlas: —

Antibody Properties

Host Species: Mouse

Clonality: Monoclonal

Isotype: MIgG2b

Myeloma Strain: NS1

Antibody Registry / RRID: AB_2314683

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)

Immunogen Sequence: Partial protein

Epitope Mapped: Yes

Epitope Location / Seq: aa 178-349 of Isl1 protein

Clone: —

Additional Info: This antibody also detects Islet-2 (Uniprot P50480. and Gene ID 57233). The protein region used for immunization lacks the LIM motifs shared with other proteins.

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish

Predicted Reactivity: —

Depositor

Depositor Name: T.M. Jessell / S. Brenner-Morton

Institution: Columbia University / HHMI

Deposit Date: November 3, 1995

Depositor Notes: MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)

Recommended Applications

ChIP

Gel Supershift

IHC

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtP61374 ↗P50480 ↗

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Could not load UniProt data for P61374

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Clone ID / Name: 39.4D5

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