39.4D5

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$40.00
SKU: 39.4D5
View product citations for antibody 39.4D5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 & Islet-2 homeobox
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish
Depositors Notes: MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Isl1 & Isl2
Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)
Alternate Gene Names: ISL1, ISL LIM homeobox 1, ISL2; ISL LIM homeobox 2
Alternate Antibody Name: anti-islet1; anti-isl1;anti-islet2; anti-isl2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 178-349 of Isl1 protein
Uniprot ID: P61374 
Immunogen Sequence: Partial protein
Entrez Gene ID: 64444 
Additional Characterization:  
Antibody Registry ID: AB_2314683 
Additional Information: This antibody also detects Islet-2 (Uniprot P50480. and Gene ID 57233). The protein region used for immunization lacks the LIM motifs shared with other proteins.
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.4D5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.4D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

51 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References

    Islet-1 regulates Arx transcription during pancreatic islet alpha-cell development.
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    IHC References

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    p57 and Hes1 coordinate cell cycle exit with self-renewal of pancreatic progenitors.
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Patterns of programmed cell death in populations of developing spinal motoneurons in chicken, mouse, and rat.
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneurons purified by a novel technique.
    deLapeyrière O
    Journal of neuroscience research 55.1 (1999 Jan 1): 119-26.

    GFRalpha 1 is required for development of distinct subpopulations of motoneuron.
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis.
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    Identification and characterization of a cell surface marker for embryonic rat spinal accessory motor neurons.
    Kaprielian Z
    The Journal of comparative neurology 439.3 (2001 Oct 22): 368-83.

    Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord.
    Takada S
    Genes & development 16.5 (2002 Mar 1): 548-53.

    The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages.
    Nabeshima Y
    Current biology : CB 12.13 (2002 Jul 9): 1157-63.

    Androgen receptors in the embryonic zebra finch hindbrain suggest a function for maternal androgens in perihatching survival.
    Gahr M
    The Journal of comparative neurology 453.1 (2002 Nov 4): 57-70.

    Electrophysiological and morphological characterization of rat embryonic motoneurons in a defined system.
    Hickman JJ
    Biotechnology progress 19.6 (2003 Nov-Dec): 1756-61.

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    Distinct domains within Mash1 and Math1 are required for function in neuronal differentiation versus neuronal cell-type specification.
    Johnson JE
    Development (Cambridge, England) 131.6 (2004 Mar): 1319-30.

    Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity.
    Sasai Y
    Proceedings of the National Academy of Sciences of the United States of America 99.3 (2002 Feb 5): 1580-5.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Mullerian inhibiting substance acts as a motor neuron survival factor in vitro.
    McLennan IS
    Proceedings of the National Academy of Sciences of the United States of America 102.45 (2005 Nov 8): 16421-5.

    Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    p57 and Hes1 coordinate cell cycle exit with self-renewal of pancreatic progenitors.
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells.
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages.
    Chien KR
    Nature 460.7251 (2009 Jul 2): 113-7.

    The Retinal Homeobox (Rx) gene is necessary for retinal regeneration.
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation.
    Takebayashi H
    Development (Cambridge, England) 139.6 (2012 Mar): 1125-32.

    LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions.
    Pfaff SL
    Cell 110.2 (2002 Jul 26): 237-49.

    Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord.
    Jessell TM
    Cell 76.4 (1994 Feb 25): 761-75.

    The control of Xenopus embryonic primary neurogenesis is mediated by retinoid signalling in the neurectoderm.
    Goldstone K
    Mechanisms of development 91.1-2 (2000 Mar 1): 69-80.

    Punc, a novel mouse gene of the immunoglobulin superfamily, is expressed predominantly in the developing nervous system.
    Salbaum JM
    Mechanisms of development 71.1-2 (1998 Feb): 201-4.

    Xenopus aristaless-related homeobox (xARX) gene product functions as both a transcriptional activator and repressor in forebrain development.
    El-Hodiri HM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.2 (2005 Feb): 313-24.

    Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification.
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Developmental restriction of the LIM homeodomain transcription factor Islet-1 expression to cholinergic neurons in the rat striatum.
    Liu FC
    Neuroscience 103.4 (2001): 999-1016.

    Molecular organization of the ferret visual thalamus.
    Katz LC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.44 (2004 Nov 3): 9962-70.

    olig2-Expressing hindbrain cells are required for migrating facial motor neurons.
    Appel B
    Developmental dynamics : an official publication of the American Association of Anatomists 241.2 (2012 Feb): 315-26.

    Identification and functional characterization of cardiac pacemaker cells in zebrafish.
    Bakkers J
    PloS one 7.10 (2012): e47644.

    Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate.
    Appel B
    Neural development 7. (2012 May 3): 15.

    prdm1a and olig4 act downstream of Notch signaling to regulate cell fate at the neural plate border.
    Artinger KB
    Developmental biology 356.2 (2011 Aug 15): 496-505.

    Neuron navigator 3a regulates liver organogenesis during zebrafish embryogenesis.
    le Noble F
    Development (Cambridge, England) 138.10 (2011 May): 1935-45.

    Ectopic proliferation contributes to retinal dysplasia in the juvenile zebrafish patched2 mutant eye.
    Gross JM
    Investigative ophthalmology & visual science 52.12 (2011 Nov 17): 8868-77.

    MicroRNA-183 family members regulate sensorineural fates in the inner ear.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.9 (2010 Mar 3): 3254-63.

    Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division.
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Suppression of Alk8-mediated Bmp signaling cell-autonomously induces pancreatic beta-cells in zebrafish.
    Stainier DY
    Proceedings of the National Academy of Sciences of the United States of America 107.3 (2010 Jan 19): 1142-7.

    DeltaA mRNA and protein distribution in the zebrafish nervous system.
    Eisen JS
    Developmental dynamics : an official publication of the American Association of Anatomists 238.12 (2009 Dec): 3226-36.

    Eye development and retinal differentiation in an altricial fish species, the senegalese sole (Solea senegalensis, Kaup 1858).
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Cell differentiation in the retina of an epibenthonic teleost, the Tench (Tinca tinca, Linneo 1758).
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals.
    Knabe W
    Biology open 7.6 (2018 Jun 19): .

    FFPE References
    Epitope Map References

    Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Gel Supershift References
    All References

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    p57 and Hes1 coordinate cell cycle exit with self-renewal of pancreatic progenitors.
    Bhushan A
    Developmental biology 298.1 (2006 Oct 1): 22-31.

    Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Patterns of programmed cell death in populations of developing spinal motoneurons in chicken, mouse, and rat.
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneurons purified by a novel technique.
    deLapeyrière O
    Journal of neuroscience research 55.1 (1999 Jan 1): 119-26.

    GFRalpha 1 is required for development of distinct subpopulations of motoneuron.
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis.
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    Identification and characterization of a cell surface marker for embryonic rat spinal accessory motor neurons.
    Kaprielian Z
    The Journal of comparative neurology 439.3 (2001 Oct 22): 368-83.

    Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord.
    Takada S
    Genes & development 16.5 (2002 Mar 1): 548-53.

    The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages.
    Nabeshima Y
    Current biology : CB 12.13 (2002 Jul 9): 1157-63.

    Androgen receptors in the embryonic zebra finch hindbrain suggest a function for maternal androgens in perihatching survival.
    Gahr M
    The Journal of comparative neurology 453.1 (2002 Nov 4): 57-70.

    Electrophysiological and morphological characterization of rat embryonic motoneurons in a defined system.
    Hickman JJ
    Biotechnology progress 19.6 (2003 Nov-Dec): 1756-61.

    Distinct domains within Mash1 and Math1 are required for function in neuronal differentiation versus neuronal cell-type specification.
    Johnson JE
    Development (Cambridge, England) 131.6 (2004 Mar): 1319-30.

    Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity.
    Sasai Y
    Proceedings of the National Academy of Sciences of the United States of America 99.3 (2002 Feb 5): 1580-5.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Mullerian inhibiting substance acts as a motor neuron survival factor in vitro.
    McLennan IS
    Proceedings of the National Academy of Sciences of the United States of America 102.45 (2005 Nov 8): 16421-5.

    Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells.
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages.
    Chien KR
    Nature 460.7251 (2009 Jul 2): 113-7.

    The Retinal Homeobox (Rx) gene is necessary for retinal regeneration.
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation.
    Takebayashi H
    Development (Cambridge, England) 139.6 (2012 Mar): 1125-32.

    LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions.
    Pfaff SL
    Cell 110.2 (2002 Jul 26): 237-49.

    Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord.
    Jessell TM
    Cell 76.4 (1994 Feb 25): 761-75.

    The control of Xenopus embryonic primary neurogenesis is mediated by retinoid signalling in the neurectoderm.
    Goldstone K
    Mechanisms of development 91.1-2 (2000 Mar 1): 69-80.

    Punc, a novel mouse gene of the immunoglobulin superfamily, is expressed predominantly in the developing nervous system.
    Salbaum JM
    Mechanisms of development 71.1-2 (1998 Feb): 201-4.

    Xenopus aristaless-related homeobox (xARX) gene product functions as both a transcriptional activator and repressor in forebrain development.
    El-Hodiri HM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.2 (2005 Feb): 313-24.

    Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification.
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Developmental restriction of the LIM homeodomain transcription factor Islet-1 expression to cholinergic neurons in the rat striatum.
    Liu FC
    Neuroscience 103.4 (2001): 999-1016.

    Molecular organization of the ferret visual thalamus.
    Katz LC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.44 (2004 Nov 3): 9962-70.

    olig2-Expressing hindbrain cells are required for migrating facial motor neurons.
    Appel B
    Developmental dynamics : an official publication of the American Association of Anatomists 241.2 (2012 Feb): 315-26.

    Identification and functional characterization of cardiac pacemaker cells in zebrafish.
    Bakkers J
    PloS one 7.10 (2012): e47644.

    Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate.
    Appel B
    Neural development 7. (2012 May 3): 15.

    prdm1a and olig4 act downstream of Notch signaling to regulate cell fate at the neural plate border.
    Artinger KB
    Developmental biology 356.2 (2011 Aug 15): 496-505.

    Neuron navigator 3a regulates liver organogenesis during zebrafish embryogenesis.
    le Noble F
    Development (Cambridge, England) 138.10 (2011 May): 1935-45.

    Ectopic proliferation contributes to retinal dysplasia in the juvenile zebrafish patched2 mutant eye.
    Gross JM
    Investigative ophthalmology & visual science 52.12 (2011 Nov 17): 8868-77.

    MicroRNA-183 family members regulate sensorineural fates in the inner ear.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.9 (2010 Mar 3): 3254-63.

    Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division.
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Suppression of Alk8-mediated Bmp signaling cell-autonomously induces pancreatic beta-cells in zebrafish.
    Stainier DY
    Proceedings of the National Academy of Sciences of the United States of America 107.3 (2010 Jan 19): 1142-7.

    DeltaA mRNA and protein distribution in the zebrafish nervous system.
    Eisen JS
    Developmental dynamics : an official publication of the American Association of Anatomists 238.12 (2009 Dec): 3226-36.

    Eye development and retinal differentiation in an altricial fish species, the senegalese sole (Solea senegalensis, Kaup 1858).
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Cell differentiation in the retina of an epibenthonic teleost, the Tench (Tinca tinca, Linneo 1758).
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals.
    Knabe W
    Biology open 7.6 (2018 Jun 19): .

    Molecular Memory of Morphologies by Septins during Neuron Generation Allows Early Polarity Inheritance.
    Castellani V
    Neuron 95.4 (2017 Aug 16): 834-851.e5.

    HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
    Suter U
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.17 (2014 Apr 23): 6112-22.

    Gfap-positive radial glial cells are an essential progenitor population for later-born neurons and glia in the zebrafish spinal cord.
    Barresi MJ
    Glia 64.7 (2016 Jul): 1170-89.

    Generation and characterization of Lhx4<sup>tdT</sup> reporter knock-in and Lhx4<sup>loxP</sup> conditional knockout mice.
    Gan L
    Genesis (New York, N.Y. : 2000) 57.10 (2019 Oct): e23328.

    <i>Dlk1-Dio3</i> locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity.
    Chen JA
    eLife 7. (2018 Oct 12): .

    Islet-1 regulates Arx transcription during pancreatic islet alpha-cell development.
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

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