39.4D5
Catalog Fields
Clone ID/Product Name: 39.4D5
Available to For-Profits: Yes
Alternate Antibody Name: anti-islet1; anti-isl1;anti-islet2; anti-isl2
Gene Symbol: Isl1 & Isl2
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2314683
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Islet-1 & Islet-2 homeobox
Epitope Location or Sequence: aa 178-349 of Isl1 protein
Alternate Antigen Name:
Deposit Date: 11/3/1995
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University / HHMI
Antigen Species: Rat
Depositor Notes: MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish
Additional Information: This antibody also detects Islet-2 (Uniprot P50480. and Gene ID 57233). The protein region used for immunization lacks the LIM motifs shared with other proteins.
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot



![Immunofluorescent image of an embryonic day 9.5 mouse section labeled with DSHB anti-Isl1/2 [39.4D5] (green). Image credit Sophie Wizniak, University of South Australia.](https://dshb.biology.uiowa.edu/SSP Applications/NetSuite Inc. - SCS/SuiteCommerce Standard/img/39.4D5_media-02.png?resizeid=3&resizeh=600&resizew=600)