F18

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SKU: F18

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DSHB Data Sheet

Catalog Fields

Product Name/ID: F18
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528369 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: myosin isolated from chicken pectoralis major muscle
Clone:
Immunogen Sequence:
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Mapped: No
Antigen Name: Myosin heavy chain, avian fast isoforms and rat, rabbit isoforms including type IIb fibers
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/1/2004
Antigen Molecular Weight:
Depositor: Stockdale, F.E.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine
Antigen Species: chicken
Depositor Notes: Species specificity note: reacts with a number of species. Characterization: specific for isoforms of myosin heavy chain associated with fast contracting muscle; recognizes multiple fast isoforms including isoforms present in avian and mammalian species. IHC note: works well on Western blots and immunostaining of tissue sections.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:  
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F18 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    All References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

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