BF-F3

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$40.00
SKU: BF-F3
View product citations for antibody BF-F3 on CiteAb

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Available: 203

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain Type IIB
Hybridoma Cells Available: Yes
Antigen Species: bovine
Depositor: Schiaffino, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova
Positive Tested Species Reactivity: Bovine, Mouse, Porcine, Rat, Sheep
Depositors Notes: Fusion date: 1983. Western Blot recognizes 2B myosin heavy chain
Antigen Molecular Weight: Predicted: 223 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYH4
Immunogen: Purified myosin from fetal bovine skeletal muscle.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: myosin heavy chain 2B
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: E1BP87 
Immunogen Sequence: Full length protein
Entrez Gene ID: 408020 
Additional Characterization:  
Antibody Registry ID: AB_2266724 
Additional Information: RRID:AB_2266724
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-F3 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

46 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    IF References

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology & visual science 41.11 (2000 Oct): 3391-8.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death & disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle & nerve 54.2 (2016 Aug): 292-9.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Synergistic PXT3003 therapy uncouples neuromuscular function from dysmyelination in male Charcot-Marie-Tooth disease type 1A (CMT1A) rats.
    Sereda MW
    Journal of neuroscience research 98.10 (2020 Oct): 1933-1952.

    Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.
    Calderó J
    Journal of cachexia, sarcopenia and muscle 11.6 (2020 Dec): 1628-1660.

    Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.
    Calderó J
    Journal of cachexia, sarcopenia and muscle 11.6 (2020 Dec): 1628-1660.

    Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading.
    Dupont-Versteegden EE
    GeroScience 43.1 (2021 Feb): 65-83.

    Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.
    Rovere-Querini P
    Aging 12.23 (2020 Nov 26): 23497-23508.

    The ROS scavenger PDTC affects adaptation to treadmill running in mice: distinct effects on murine body mass, resting heart rate and skeletal muscle fiber type composition.
    Munz B
    The Journal of experimental biology 224.Pt 6 (2021 Mar 18): .

    Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice.
    Shanely RA
    International journal of environmental research and public health 18.2 (2021 Jan 6): .

    Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2β.
    Gregorevic P
    Molecular & cellular proteomics : MCP 20. (2021 Jan 29): 100050.

    Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy.
    Höke A
    Scientific data 6.1 (2019 Sep 24): 179.

    Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice.
    Cornachione AS
    Scientific reports 11.1 (2021 Feb 11): 3579.

    WB References
    IHC References

    New insights into muscle fiber types in the pig.
    Gueguen N
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.5 (2002 May): 719-30.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans.
    Bell GJ
    European journal of applied physiology 92.4-5 (2004 Aug): 376-84.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Myosin heavy chain 2B isoform is expressed in specialized eye muscles but not in trunk and limb muscles of cattle.
    Mascarello F
    European journal of histochemistry : EJH 48.4 (2004 Oct-Dec): 357-66.

    Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.
    Gorza L
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.2 (1990 Feb): 257-65.

    Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD.
    Levine S
    Journal of applied physiology (Bethesda, Md. : 1985) 88.4 (2000 Apr): 1446-56.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.
    Kurland IJ
    Veterinary pathology 56.2 (2019 Mar): 322-331.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    Adenosine/A2B Receptor Signaling Ameliorates the Effects of Aging and Counteracts Obesity.
    Pfeifer A
    Cell metabolism 32.1 (2020 Jul 7): 56-70.e7.

    Regular endurance exercise of overloaded muscle of young and old male mice does not attenuate hypertrophy and improves fatigue resistance.
    Degens H
    GeroScience . (2020 Jul 8): .

    Cardiotoxin-induced skeletal muscle injury elicits profound changes in anabolic and stress signaling, and muscle fiber type composition.
    Koppo K
    Journal of muscle research and cell motility 41.4 (2020 Dec): 375-387.

    Maintenance of contractile force and increased fatigue resistance in slow-twitch skeletal muscle of mice fed a high-fat diet.
    Watada H
    Journal of applied physiology (Bethesda, Md. : 1985) 130.3 (2021 Mar 1): 528-536.

    Mss51 deletion increases endurance and ameliorates histopathology in the mdx mouse model of Duchenne muscular dystrophy.
    Wagner KR
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.2 (2021 Feb): e21276.

    <i>Citrus hassaku</i> Extract Powder Increases Mitochondrial Content and Oxidative Muscle Fibers by Upregulation of PGC-1α in Skeletal Muscle.
    Miura S
    Nutrients 13.2 (2021 Feb 3): .

    Single-leg exercise training augments in vivo skeletal muscle oxidative flux and vascular content and function in adults with type 2 diabetes.
    Regensteiner JG
    The Journal of physiology . (2021 Feb 10): .

    Home apnea monitoring.
    Hutchins KH
    Pediatric nursing 12.4 (1986 Jul-Aug): 259-62, 304.

    ELISA References

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    All References

    New insights into muscle fiber types in the pig.
    Gueguen N
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.5 (2002 May): 719-30.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans.
    Bell GJ
    European journal of applied physiology 92.4-5 (2004 Aug): 376-84.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Myosin heavy chain 2B isoform is expressed in specialized eye muscles but not in trunk and limb muscles of cattle.
    Mascarello F
    European journal of histochemistry : EJH 48.4 (2004 Oct-Dec): 357-66.

    Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.
    Gorza L
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.2 (1990 Feb): 257-65.

    Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD.
    Levine S
    Journal of applied physiology (Bethesda, Md. : 1985) 88.4 (2000 Apr): 1446-56.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.
    Kurland IJ
    Veterinary pathology 56.2 (2019 Mar): 322-331.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    Adenosine/A2B Receptor Signaling Ameliorates the Effects of Aging and Counteracts Obesity.
    Pfeifer A
    Cell metabolism 32.1 (2020 Jul 7): 56-70.e7.

    Regular endurance exercise of overloaded muscle of young and old male mice does not attenuate hypertrophy and improves fatigue resistance.
    Degens H
    GeroScience . (2020 Jul 8): .

    Cardiotoxin-induced skeletal muscle injury elicits profound changes in anabolic and stress signaling, and muscle fiber type composition.
    Koppo K
    Journal of muscle research and cell motility 41.4 (2020 Dec): 375-387.

    Maintenance of contractile force and increased fatigue resistance in slow-twitch skeletal muscle of mice fed a high-fat diet.
    Watada H
    Journal of applied physiology (Bethesda, Md. : 1985) 130.3 (2021 Mar 1): 528-536.

    Mss51 deletion increases endurance and ameliorates histopathology in the mdx mouse model of Duchenne muscular dystrophy.
    Wagner KR
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.2 (2021 Feb): e21276.

    <i>Citrus hassaku</i> Extract Powder Increases Mitochondrial Content and Oxidative Muscle Fibers by Upregulation of PGC-1α in Skeletal Muscle.
    Miura S
    Nutrients 13.2 (2021 Feb 3): .

    Single-leg exercise training augments in vivo skeletal muscle oxidative flux and vascular content and function in adults with type 2 diabetes.
    Regensteiner JG
    The Journal of physiology . (2021 Feb 10): .

    Home apnea monitoring.
    Hutchins KH
    Pediatric nursing 12.4 (1986 Jul-Aug): 259-62, 304.

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology & visual science 41.11 (2000 Oct): 3391-8.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death & disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle & nerve 54.2 (2016 Aug): 292-9.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Synergistic PXT3003 therapy uncouples neuromuscular function from dysmyelination in male Charcot-Marie-Tooth disease type 1A (CMT1A) rats.
    Sereda MW
    Journal of neuroscience research 98.10 (2020 Oct): 1933-1952.

    Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.
    Calderó J
    Journal of cachexia, sarcopenia and muscle 11.6 (2020 Dec): 1628-1660.

    Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading.
    Dupont-Versteegden EE
    GeroScience 43.1 (2021 Feb): 65-83.

    Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.
    Rovere-Querini P
    Aging 12.23 (2020 Nov 26): 23497-23508.

    The ROS scavenger PDTC affects adaptation to treadmill running in mice: distinct effects on murine body mass, resting heart rate and skeletal muscle fiber type composition.
    Munz B
    The Journal of experimental biology 224.Pt 6 (2021 Mar 18): .

    Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice.
    Shanely RA
    International journal of environmental research and public health 18.2 (2021 Jan 6): .

    Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2β.
    Gregorevic P
    Molecular & cellular proteomics : MCP 20. (2021 Jan 29): 100050.

    Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy.
    Höke A
    Scientific data 6.1 (2019 Sep 24): 179.

    Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice.
    Cornachione AS
    Scientific reports 11.1 (2021 Feb 11): 3579.

    Spatial distribution of myosin heavy-chain isoforms in mouse masseter.
    Nekula C
    Journal of dental research 81.1 (2002 Jan): 33-8.

    A maternal high-fat/low-fiber diet impairs glucose tolerance and induces the formation of glycolytic muscle fibers in neonatal offspring.
    Tan C
    European journal of nutrition . (2021 Jan 2): .

    A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.
    Zhang ZJ
    Acta neuropathologica communications 9.1 (2021 Feb 8): 23.

    MicroRNA-129-5p Inhibited C2C12 Myogenesis and Repressed Slow Fiber Gene Expression in vitro.
    Li X
    American journal of physiology. Cell physiology . (2021 Apr 7): .

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