BF-F3

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$40.00
SKU: BF-F3
View product citations for antibody BF-F3 on CiteAb

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Available: 421

DSHB Data Sheet

Catalog Fields

Antigen: Myosin Heavy Chain Type IIB
Hybridoma Cells Available: Yes
Antigen Species: bovine
Depositor: Schiaffino, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova
Positive Tested Species Reactivity: Bovine, Mouse, Porcine, Rat, Sheep
Depositors Notes: Fusion date: 1983. Western Blot recognizes 2B myosin heavy chain
Antigen Molecular Weight: Predicted: 223 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH4
Immunogen: Purified myosin from fetal bovine skeletal muscle.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: myosin heavy chain 2B
Epitope Mapped:
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: E1BP87 
Immunogen Sequence: Full length protein
Entrez Gene ID: 408020 
Additional Characterization:
Antibody Registry ID: AB_2266724 
Additional Information: RRID:AB_2266724
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-F3 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    IF References

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology and visual science 41.11 (2000 Oct): 3391-8.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death and disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle and nerve 54.2 (2016 Aug): 292-9.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    WB References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Spatial distribution of myosin heavy-chain isoforms in mouse masseter.
    Nekula C
    Journal of dental research 81.1 (2002 Jan): 33-8.

    IHC References

    New insights into muscle fiber types in the pig.
    Gueguen N
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.5 (2002 May): 719-30.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans.
    Bell GJ
    European journal of applied physiology 92.4-5 (2004 Aug): 376-84.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Myosin heavy chain 2B isoform is expressed in specialized eye muscles but not in trunk and limb muscles of cattle.
    Mascarello F
    European journal of histochemistry : EJH 48.4 (2004 Oct-Dec): 357-66.

    Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.
    Gorza L
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.2 (1990 Feb): 257-65.

    Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD.
    Levine S
    Journal of applied physiology (Bethesda, Md. : 1985) 88.4 (2000 Apr): 1446-56.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.
    Kurland IJ
    Veterinary pathology 56.2 (2019 Mar): 322-331.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    ELISA References

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    All References

    New insights into muscle fiber types in the pig.
    Gueguen N
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.5 (2002 May): 719-30.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans.
    Bell GJ
    European journal of applied physiology 92.4-5 (2004 Aug): 376-84.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Myosin heavy chain 2B isoform is expressed in specialized eye muscles but not in trunk and limb muscles of cattle.
    Mascarello F
    European journal of histochemistry : EJH 48.4 (2004 Oct-Dec): 357-66.

    Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.
    Gorza L
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 38.2 (1990 Feb): 257-65.

    Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD.
    Levine S
    Journal of applied physiology (Bethesda, Md. : 1985) 88.4 (2000 Apr): 1446-56.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.
    Kurland IJ
    Veterinary pathology 56.2 (2019 Mar): 322-331.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Myosin isoform expression in rat rhabdomyosarcoma induced by Moloney murine sarcoma virus.
    Schiaffino S
    Journal of cancer research and clinical oncology 113.5 (1987): 417-29.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology and visual science 41.11 (2000 Oct): 3391-8.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death and disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle and nerve 54.2 (2016 Aug): 292-9.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Spatial distribution of myosin heavy-chain isoforms in mouse masseter.
    Nekula C
    Journal of dental research 81.1 (2002 Jan): 33-8.

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