H5A4

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$45.00 to $425.00
SKU: H5A4
View product citations for antibody H5A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: August, J.T. / Hildreth, J.E.K.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Johns Hopkins University School of Medicine
Antigen Molecular Weight: Predicted: 127 kDa; Apparent: 170 kDa
Depositors Notes: Does not work on pig. Blocks leukocyte adhesion and inhibits T cell activity; no effect on NK cell activity. Stains frozen sections of human tonsil. Binds to PBMC.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Adult Human adherent spleen cells
Gene: ITGAM
Alternate Antibody Name:
Alternate Gene Names: MAC-1, MO1A, CR3
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X653-Ag8
Epitope Mapped: No
Uniprot ID: P11215 
Epitope Location or Sequence:
Entrez Gene ID: 3684 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528134 
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Mac-1 is a macrophage differenciation antigen and a marker for microglia.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5A4 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H5A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References
    FB References
    FACS References

    Expression and function of P-selectin glycoprotein ligand 1 (CD162) on human basophils.
    Bochner BS
    The Journal of allergy and clinical immunology 106.5 (2000 Nov): 918-24.

    All References

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

    The role of complement receptor 3 (CR3) in Neisseria gonorrhoeae infection of human cervical epithelia.
    Apicella MA
    Cellular microbiology 3.9 (2001 Sep): 611-22.

    Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Schlesinger LS
    mBio 6.1 (2015 Feb 17): e02537-14.

    Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.
    Wilson ME
    Cellular microbiology 11.12 (2009 Dec): 1827-41.

    The role of lipooligosaccharide in Neisseria gonorrhoeae pathogenesis of cervical epithelia: lipid A serves as a C3 acceptor molecule.
    Apicella MA
    Cellular microbiology 4.9 (2002 Sep): 585-98.

    Neisseria gonorrhoeae PLD directly interacts with Akt kinase upon infection of primary, human, cervical epithelial cells.
    Apicella MA
    Cellular microbiology 8.8 (2006 Aug): 1253-71.

    Gonococcal phospholipase d modulates the expression and function of complement receptor 3 in primary cervical epithelial cells.
    Apicella MA
    Infection and immunity 71.11 (2003 Nov): 6381-91.

    Expression and function of P-selectin glycoprotein ligand 1 (CD162) on human basophils.
    Bochner BS
    The Journal of allergy and clinical immunology 106.5 (2000 Nov): 918-24.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

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