H5A4

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$40.00
SKU: H5A4
View product citations for antibody H5A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: Does not work on pig. Blocks leukocyte adhesion and inhibits T cell activity; no effect on NK cell activity. Stains frozen sections of human tonsil. Binds to PBMC.
Antigen Molecular Weight: Predicted: 127 kDa; Apparent: 170 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGAM
Immunogen: Adult Human adherent spleen cells
Alternate Gene Names: MAC-1, MO1A, CR3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence:
Uniprot ID: P11215 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3684 
Additional Characterization:
Antibody Registry ID: AB_528134 
Additional Information: Mac-1 is a macrophage differenciation antigen and a marker for microglia.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5A4 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H5A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References
    FB References
    FACS References

    Expression and function of P-selectin glycoprotein ligand 1 (CD162) on human basophils.
    Bochner BS
    The Journal of allergy and clinical immunology 106.5 (2000 Nov): 918-24.

    All References

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

    The role of complement receptor 3 (CR3) in Neisseria gonorrhoeae infection of human cervical epithelia.
    Apicella MA
    Cellular microbiology 3.9 (2001 Sep): 611-22.

    Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Schlesinger LS
    mBio 6.1 (2015 Feb 17): e02537-14.

    Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.
    Wilson ME
    Cellular microbiology 11.12 (2009 Dec): 1827-41.

    The role of lipooligosaccharide in Neisseria gonorrhoeae pathogenesis of cervical epithelia: lipid A serves as a C3 acceptor molecule.
    Apicella MA
    Cellular microbiology 4.9 (2002 Sep): 585-98.

    Neisseria gonorrhoeae PLD directly interacts with Akt kinase upon infection of primary, human, cervical epithelial cells.
    Apicella MA
    Cellular microbiology 8.8 (2006 Aug): 1253-71.

    Gonococcal phospholipase d modulates the expression and function of complement receptor 3 in primary cervical epithelial cells.
    Apicella MA
    Infection and immunity 71.11 (2003 Nov): 6381-91.

    Expression and function of P-selectin glycoprotein ligand 1 (CD162) on human basophils.
    Bochner BS
    The Journal of allergy and clinical immunology 106.5 (2000 Nov): 918-24.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

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