H5A5

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$40.00
SKU: H5A5
View product citations for antibody H5A5 on CiteAb

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Available: 43

DSHB Data Sheet

Catalog Fields

Antigen: Receptor-type tyrosine-protein phosphatase C
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as CD45- lymphocyte common antigen.
Antigen Molecular Weight: 147.4 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PTPRC
Immunogen: Adherent spleen cells
Alternate Gene Names: CD45
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence:
Uniprot ID: P08575 
Immunogen Sequence: Full length protein
Entrez Gene ID: 5788 
Additional Characterization:  
Antibody Registry ID: AB_528150 
Additional Information: RRID:AB_528150
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5A5 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IHC References
  • ELISA References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IHC References

    A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
    Engle EC
    Nature communications 8. (2017 Jul 6): 16077.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    ELISA References

    Association of host cell surface adhesion receptors and other membrane proteins with HIV and SIV.
    Hildreth JE
    AIDS research and human retroviruses 9.11 (1993 Nov): 1157-65.

    FFPE References

    A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
    Engle EC
    Nature communications 8. (2017 Jul 6): 16077.

    FACS References
    All References

    A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
    Engle EC
    Nature communications 8. (2017 Jul 6): 16077.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation.
    Kawakami K
    Development, growth & differentiation 58.6 (2016 Aug): 530-45.

    VEGF receptor blockade markedly reduces retinal microglia/macrophage infiltration into laser-induced CNV.
    Vinores SA
    PloS one 8.8 (2013): e71808.

    Anti-class II MHC antibody induces multinucleated giant cell formation from peripheral blood monocytes.
    Hildreth JE
    Journal of leukocyte biology 51.3 (1992 Mar): 199-209.

    Association of host cell surface adhesion receptors and other membrane proteins with HIV and SIV.
    Hildreth JE
    AIDS research and human retroviruses 9.11 (1993 Nov): 1157-65.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

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