H5A5
Catalog Fields
Clone ID/Product Name: H5A5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: PTPRC
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 5788
Clonality: Monoclonal
Immunogen: Adherent spleen cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X653-Ag8
Epitope Mapped: No
Antigen Name: Receptor-type tyrosine-protein phosphatase C
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/12/1993
Antigen Molecular Weight: 147.4 kDa
Depositor: August, J.T. / Hildreth, J.E.K.
Antigen Sequence:
Depositor Institution: Johns Hopkins University School of Medicine
Antigen Species: Human
Depositor Notes: This antigen is also known as CD45- lymphocyte common antigen.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_528150
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5A5 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
August JT
Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.