HCS-1

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SKU: HCS-1

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: HCS-1
Available to For-Profits: Yes
Alternate Antibody Name: otoferlin
Gene Symbol: OTOF
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_10804296 
Uniprot ID: Q9HC10 
RRID:  
Entrez Gene ID: 9381 
Clonality: Monoclonal
Immunogen: Delaminated chick utricles emulsion
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: SP/0
Epitope Mapped: No
Antigen Name: Otoferlin; Hair cell soma-1
Epitope Location or Sequence:
Alternate Antigen Name: otoferlin
Deposit Date: 11/16/2010
Antigen Molecular Weight: 210 kDa /230 kDa
Depositor: Corwin, J.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Chicken
Depositor Notes: This antigen is a key calcium ion sensor regulating neurotransmitter release. The antigen is also known as Fer-1-like protein 2.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Amphibian, Avian, Elasmobranch, Fish, Mammal, Reptile
Additional Information: RRID:AB_10804296
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HCS-1 was deposited to the DSHB by Corwin, J. (DSHB Hybridoma Product HCS-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    IF References

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    Expression and Misexpression of the miR-183 Family in the Developing Hearing Organ of the Chicken.
    Fekete DM
    PloS one 10.7 (2015): e0132796.

    Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance.
    Perkins BD
    Scientific reports 9.1 (2019 Jul 16): 10302.

    IHC References

    Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish.
    Lecaudey V
    eLife 4. (2015 Sep 3): e08201.

    Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 37.37 (2017 Sep 13): 8975-8988.

    IP References

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    All References

    Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish.
    Lecaudey V
    eLife 4. (2015 Sep 3): e08201.

    Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 37.37 (2017 Sep 13): 8975-8988.

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    Expression and Misexpression of the miR-183 Family in the Developing Hearing Organ of the Chicken.
    Fekete DM
    PloS one 10.7 (2015): e0132796.

    Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance.
    Perkins BD
    Scientific reports 9.1 (2019 Jul 16): 10302.

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