MYO7A 138-1

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$50.00
SKU: MYO7A 138-1

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: MYO7A 138-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYO7A
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2282417 
Uniprot ID: Q13402 
RRID:  
Entrez Gene ID: 4647 
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: a.a. 927-1203
Myeloma Strain: MOPC-21 NS-1
Epitope Mapped: Yes
Antigen Name: Unconventional myosin-VIIa
Epitope Location or Sequence: a.a. 927-1203
Alternate Antigen Name:
Deposit Date: 11/1/2007
Antigen Molecular Weight: 240 kDa
Depositor: Orten, D.J.
Antigen Sequence:
Depositor Institution: Boys Town National Research Hospital
Antigen Species: Human
Depositor Notes: Mutations in this antigen lead to the human condition Usher syndrome 1B.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Mouse, Rat, Xenopus, Zebrafish
Additional Information: RRID:AB_2282417
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MYO7A 138-1 was deposited to the DSHB by Orten, D.J. (DSHB Hybridoma Product MYO7A 138-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The unconventional myosin-VIIa associates with lysosomes.
    Hasson T
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 13-26.

    IF References
    WB References

    The unconventional myosin-VIIa associates with lysosomes.
    Hasson T
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 13-26.

    IHC References
    IP References

    The unconventional myosin-VIIa associates with lysosomes.
    Hasson T
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 13-26.

    Epitope Map References

    The unconventional myosin-VIIa associates with lysosomes.
    Hasson T
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 13-26.

    All References

    MYC gene delivery to adult mouse utricles stimulates proliferation of postmitotic supporting cells in vitro.
    Jackson JD
    PloS one 7.10 (2012): e48704.

    Dual embryonic origin of the mammalian otic vesicle forming the inner ear.
    Morrow BE
    Development (Cambridge, England) 138.24 (2011 Dec): 5403-14.

    Cisplatin is retained in the cochlea indefinitely following chemotherapy.
    Cunningham LL
    Nature communications 8.1 (2017 Nov 21): 1654.

    Simultaneous gentamicin-mediated damage and Atoh1 overexpression promotes hair cell regeneration in the neonatal mouse utricle.
    Ren D
    Experimental cell research 398.1 (2021 Jan 1): 112395.

    Naringin attenuates cisplatin- and aminoglycoside-induced hair cell injury in the zebrafish lateral line via multiple pathways.
    Lu Z
    Journal of cellular and molecular medicine 25.2 (2021 Jan): 975-989.

    The unconventional myosin-VIIa associates with lysosomes.
    Hasson T
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 13-26.

    Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases.
    Burgess SM
    Scientific reports 6. (2016 Jul 18): 29946.

    Efferent innervation of turtle semicircular canal cristae: comparisons with bird and mouse.
    Holt JC
    The Journal of comparative neurology 523.8 (2015 Jun 1): 1258-80.

    Expression and Localization of CaBP Ca2+ Binding Proteins in the Mouse Cochlea.
    Lee A
    PloS one 11.1 (2016): e0147495.

    MET currents and otoacoustic emissions from mice with a detached tectorial membrane indicate the extracellular matrix regulates Ca(2+) near stereocilia.
    Marcotti W
    The Journal of physiology 599.7 (2021 Apr): 2015-2036.

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