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View product citations for antibody MANLAC1(4A7) on CiteAb

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Available: 105

DSHB Data Sheet

Catalog Fields

Antigen: Lamin A/C
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2a, kappa light chain
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Host Species: mouse
Depositors Notes: Fixation with acetone/methanol is recommended.
Positive Tested Species Reactivity: Chinese Hamster, Human, Mouse, Rabbit
Gene: LMNA
Antigen Molecular Weight: 71 kda
Alternate Gene Names: LMN1
Immunogen: Recombinant human pre-lamin A expressed in E. coli
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: P02545 
Epitope Location or Sequence: PLLTYRFPP
Entrez Gene ID: 4000 
Immunogen Sequence: Full length
Antibody Registry ID: AB_2618203 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information: RRID:AB_2618203
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANLAC1(4A7) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANLAC1(4A7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

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