Catalog Fields
Antigen: Green fluorescent protein (GFP)
Available to For-Profits: Yes
Antigen Species: Aequorea victoria
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1, kappa light chain
Depositor: DSHB
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Aequorea victoria, All species for fusion proteins
Depositors Institution: University of Iowa
Antigen Molecular Weight: 26.8 kDa
Depositors Notes: DSHB-GFP-12A6 does not western blot.
Immunogen: Recombinant protein
Gene: GFP
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Uniprot ID: P42212
Epitope Location or Sequence:
Antibody Registry ID: AB_2617417
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Additional Information: 12A6 also recognizes eGFP and BFP but not mCherry or RFP as determined by ELISA. RRID:AB_2617417
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-12A6 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-12A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant.
Soll DR
Monoclonal antibodies in immunodiagnosis and immunotherapy 33.2 (2014 Apr): 80-8.