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DSHB Data Sheet

Catalog Fields

Product Name/ID: DSHB-GFP-12A6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: GFP
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617417 
Uniprot ID: P42212 
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Recombinant protein
Immunogen Sequence: Full length
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: Green fluorescent protein (GFP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/15/2013
Antigen Molecular Weight: 26.8 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Aequorea victoria
Depositor Notes: DSHB-GFP-12A6 does not western blot.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Aequorea victoria, All species for fusion proteins
Additional Information: 12A6 also recognizes eGFP and BFP but not mCherry or RFP as determined by ELISA. RRID:AB_2617417
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-12A6 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-12A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IP References
  • ChIP References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IP References
    ChIP References
    FFPE References
    FACS References
    All References

    Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.2 (2014 Apr): 80-8.

    Netrin-1 induces local translation of down syndrome cell adhesion molecule in axonal growth cones.
    Welshhans K
    Developmental neurobiology 76.7 (2016 Jul): 799-816.

    The defender against apoptotic cell death 1 gene is required for tissue growth and efficient N-glycosylation in Drosophila melanogaster.
    Lai ZC
    Developmental biology 420.1 (2016 Dec 1): 186-195.

    LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans.
    Greenstein D
    Genetics 206.4 (2017 Aug): 2007-2039.

    A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.
    Fulga TA
    Nature communications 8.1 (2017 Nov 21): 1663.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization.
    Grassl GA
    PLoS pathogens 15.7 (2019 Jul): e1007915.

    Mitochondrial localization of Dictyostelium discoideum dUTPase mediated by its N-terminus.
    Moriyama H
    BMC research notes 13.1 (2020 Jan 7): 16.

    Epigenetic regulation of hematopoiesis by DNA methylation.
    Weinstein BM
    eLife 5. (2016 Jan 27): e11813.

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