JLA20

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$40.00
SKU: JLA20
View product citations for antibody JLA20 on CiteAb

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Available: 264

DSHB Data Sheet

Catalog Fields

Antigen: actin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgM, kappa light chain
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: This antibody recognizes all isoforms of actin over a broad range of species.
Positive Tested Species Reactivity: Bacteria, Chicken, Drosophila, Fungi, Human, Mouse, Planaria, Plant, Rat, Xenopus, Zebrafish
Gene: ACTA
Antigen Molecular Weight: 43 kDa
Clonality: Monoclonal
Immunogen: Gizzard lysate
Epitope Mapped: No
Myeloma Strain: NS1
Immunogen Sequence: Cytoskeletal preparation
Uniprot ID: P68139 
Entrez Gene ID: 423787 
Antibody Registry ID: AB_528068 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLA20 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLA20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.
    Lin JJ
    Proceedings of the National Academy of Sciences of the United States of America 78.4 (1981 Apr): 2335-9.

    IF References

    Fluorescence microscopic localization of actin in pollen tubes: comparison of actin antibody and phalloidin staining.
    Hepler PK
    Cell motility and the cytoskeleton 12.4 (1989): 216-24.

    Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.
    Lin JJ
    Proceedings of the National Academy of Sciences of the United States of America 78.4 (1981 Apr): 2335-9.

    Fluorescence microscopic localization of actin in pollen tubes: comparison of actin antibody and phalloidin staining.
    Hepler PK
    Cell motility and the cytoskeleton 12.4 (1989): 216-24.

    A class-V myosin required for mating, hyphal growth, and pathogenicity in the dimorphic plant pathogen Ustilago maydis.
    Steinberg G
    The Plant cell 15.12 (2003 Dec): 2826-42.

    α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton.
    Feany MB
    Neuron 97.1 (2018 Jan 3): 108-124.e6.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Impaired glucose tolerance, glucagon, and insulin responses in mice lacking the loop diuretic-sensitive <i>Nkcc2a</i> transporter.
    Di Fulvio M
    American journal of physiology. Cell physiology 317.4 (2019 Oct 1): C843-C856.

    Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy.
    Babu GJ
    American journal of physiology. Cell physiology 317.4 (2019 Oct 1): C813-C824.

    WB References

    Biochemical and immunological characterization of pea nuclear intermediate filament proteins.
    Roux SJ
    Planta 218.6 (2004 Apr): 965-75.

    A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities.
    Yamaguchi M
    Experimental cell research 362.2 (2018 Jan 15): 461-471.

    ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa.
    Rietsch A
    Journal of bacteriology 190.8 (2008 Apr): 2726-38.

    TFEB Overexpression in the P301S Model of Tauopathy Mitigates Increased PHF1 Levels and Lipofuscin Puncta and Rescues Memory Deficits.
    Lakshmana MK
    eNeuro 3.2 (2016 Mar-Apr): .

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease.
    Cho KS
    Disease models & mechanisms 9.3 (2016 Mar): 295-306.

    Tumor suppressive role of sestrin2 during colitis and colon carcinogenesis.
    Lee JH
    eLife 5. (2016 Feb 25): e12204.

    IL-17A Promotes Intracellular Growth of Mycobacterium by Inhibiting Apoptosis of Infected Macrophages.
    Castro AG
    Frontiers in immunology 6. (2015): 498.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Lrrk promotes tau neurotoxicity through dysregulation of actin and mitochondrial dynamics.
    Feany MB
    PLoS biology 16.12 (2018 Dec): e2006265.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death & disease 10.8 (2019 Jul 22): 564.

    PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer.
    Strömblad S
    Nature communications 10.1 (2019 Aug 9): 3589.

    IP References
    All References

    Fluorescence microscopic localization of actin in pollen tubes: comparison of actin antibody and phalloidin staining.
    Hepler PK
    Cell motility and the cytoskeleton 12.4 (1989): 216-24.

    Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.
    Lin JJ
    Proceedings of the National Academy of Sciences of the United States of America 78.4 (1981 Apr): 2335-9.

    A class-V myosin required for mating, hyphal growth, and pathogenicity in the dimorphic plant pathogen Ustilago maydis.
    Steinberg G
    The Plant cell 15.12 (2003 Dec): 2826-42.

    α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton.
    Feany MB
    Neuron 97.1 (2018 Jan 3): 108-124.e6.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Impaired glucose tolerance, glucagon, and insulin responses in mice lacking the loop diuretic-sensitive <i>Nkcc2a</i> transporter.
    Di Fulvio M
    American journal of physiology. Cell physiology 317.4 (2019 Oct 1): C843-C856.

    Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy.
    Babu GJ
    American journal of physiology. Cell physiology 317.4 (2019 Oct 1): C813-C824.

    Biochemical and immunological characterization of pea nuclear intermediate filament proteins.
    Roux SJ
    Planta 218.6 (2004 Apr): 965-75.

    A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities.
    Yamaguchi M
    Experimental cell research 362.2 (2018 Jan 15): 461-471.

    ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa.
    Rietsch A
    Journal of bacteriology 190.8 (2008 Apr): 2726-38.

    TFEB Overexpression in the P301S Model of Tauopathy Mitigates Increased PHF1 Levels and Lipofuscin Puncta and Rescues Memory Deficits.
    Lakshmana MK
    eNeuro 3.2 (2016 Mar-Apr): .

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease.
    Cho KS
    Disease models & mechanisms 9.3 (2016 Mar): 295-306.

    Tumor suppressive role of sestrin2 during colitis and colon carcinogenesis.
    Lee JH
    eLife 5. (2016 Feb 25): e12204.

    IL-17A Promotes Intracellular Growth of Mycobacterium by Inhibiting Apoptosis of Infected Macrophages.
    Castro AG
    Frontiers in immunology 6. (2015): 498.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Lrrk promotes tau neurotoxicity through dysregulation of actin and mitochondrial dynamics.
    Feany MB
    PLoS biology 16.12 (2018 Dec): e2006265.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death & disease 10.8 (2019 Jul 22): 564.

    PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer.
    Strömblad S
    Nature communications 10.1 (2019 Aug 9): 3589.

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    Structure of vimentin 10-nm filaments probed with a monoclonal antibody that recognizes a common antigenic determinant on vimentin and tropomyosin.
    Lin JJ
    Cold Spring Harbor symposia on quantitative biology 46 Pt 1. (1982): 455-63.

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