JLA20
Catalog Fields
Antigen: actin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgM, kappa light chain
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: This antibody recognizes all isoforms of actin over a broad range of species.
Positive Tested Species Reactivity: Bacteria, Chicken, Drosophila, Fungi, Human, Mouse, Planaria, Plant, Rat, Xenopus, Zebrafish
Gene: ACTA
Antigen Molecular Weight: 43 kDa
Clonality: Monoclonal
Immunogen: Gizzard lysate
Epitope Mapped: No
Myeloma Strain: NS1
Immunogen Sequence: Cytoskeletal preparation
Uniprot ID: P68139
Entrez Gene ID: 423787
Antibody Registry ID: AB_528068
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLA20 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLA20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
21 References
Initial Publication
Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.
Lin JJ
Proceedings of the National Academy of Sciences of the United States of America 78.4 (1981 Apr): 2335-9.