MJ7/18

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$40.00
SKU: MJ7/18
View product citations for antibody MJ7/18 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: endoglin (CD105)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Butcher, E.C. / Jutila, M.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Stanford University School of Medicine and Montana State University
Positive Tested Species Reactivity: Mouse
Depositors Notes: Pan endothelial marker with an extremely weak (minimal) reactivity with early hematopoetic cells (J. Spangrude, personal communication).
Antigen Molecular Weight: Predicted: 70 kDa; Apparent: 180 kDa (nonreduced) or 90 (reduced)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Eng
Immunogen: Inflamed mouse skin
Alternate Gene Names: CD105, Edg, Endo
Alternate Antibody Name: CD105
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q63961 
Immunogen Sequence: Full length protein
Entrez Gene ID: 13805 
Additional Characterization:  
Antibody Registry ID: AB_2262267 
Additional Information: Endoglin is a major glycoprotein of vascular endothelium. MJ7/18 is commonly used for the isolation of murine endothelial cells.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MJ7/18 was deposited to the DSHB by Butcher, E.C. / Jutila, M. (DSHB Hybridoma Product MJ7/18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Specialized patterns of vascular differentiation antigens in the pregnant mouse uterus and the placenta.
    Butcher EC
    Biology of reproduction 61.6 (1999 Dec): 1393-401.

    Targeting vascular endothelium with avidin microbubbles.
    Brekken RA
    Ultrasound in medicine & biology 31.9 (2005 Sep): 1279-83.

    Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature.
    Brekken RA
    Clinical cancer research : an official journal of the American Association for Cancer Research 13.1 (2007 Jan 1): 323-30.

    SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-β1 activity.
    Brekken RA
    The Journal of cell biology 193.7 (2011 Jun 27): 1305-19.

    Endoglin regulates cancer-stromal cell interactions in prostate tumors.
    Vary CP
    Cancer research 71.10 (2011 May 15): 3482-93.

    Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.
    Eisch AJ
    Neuroscience 159.3 (2009 Mar 31): 1003-10.

    Comparative study of stromal cell lines derived from embryonic, fetal, and postnatal mouse blood-forming tissues.
    Péault B
    Experimental hematology 30.10 (2002 Oct): 1202-10.

    WB References
    IHC References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    Cell adhesion molecules on vessels during inflammation in the mouse central nervous system.
    Butcher EC
    Journal of neuroimmunology 51.2 (1994 May): 199-208.

    Endoglin regulates cancer-stromal cell interactions in prostate tumors.
    Vary CP
    Cancer research 71.10 (2011 May 15): 3482-93.

    Effect of vascular endothelial growth factor on functional recovery after hepatectomy in lean and obese mice.
    Dufour JF
    Journal of hepatology 40.2 (2004 Feb): 305-12.

    IP References

    SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-β1 activity.
    Brekken RA
    The Journal of cell biology 193.7 (2011 Jun 27): 1305-19.

    FACS References
    Epitope Map References
    All References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    Cell adhesion molecules on vessels during inflammation in the mouse central nervous system.
    Butcher EC
    Journal of neuroimmunology 51.2 (1994 May): 199-208.

    Endoglin regulates cancer-stromal cell interactions in prostate tumors.
    Vary CP
    Cancer research 71.10 (2011 May 15): 3482-93.

    Effect of vascular endothelial growth factor on functional recovery after hepatectomy in lean and obese mice.
    Dufour JF
    Journal of hepatology 40.2 (2004 Feb): 305-12.

    Specialized patterns of vascular differentiation antigens in the pregnant mouse uterus and the placenta.
    Butcher EC
    Biology of reproduction 61.6 (1999 Dec): 1393-401.

    Targeting vascular endothelium with avidin microbubbles.
    Brekken RA
    Ultrasound in medicine & biology 31.9 (2005 Sep): 1279-83.

    Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature.
    Brekken RA
    Clinical cancer research : an official journal of the American Association for Cancer Research 13.1 (2007 Jan 1): 323-30.

    SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-β1 activity.
    Brekken RA
    The Journal of cell biology 193.7 (2011 Jun 27): 1305-19.

    Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.
    Eisch AJ
    Neuroscience 159.3 (2009 Mar 31): 1003-10.

    Comparative study of stromal cell lines derived from embryonic, fetal, and postnatal mouse blood-forming tissues.
    Péault B
    Experimental hematology 30.10 (2002 Oct): 1202-10.

    Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand.
    Butcher EC
    Gene 138.1-2 (1994 Jan 28): 201-6.

    Conditional immortalization of growth factor-responsive cardiac endothelial cells from H-2K(b)-tsA58 mice.
    Mason JC
    American journal of physiology. Cell physiology 282.1 (2002 Jan): C67-74.

    Expression profile of active genes in mouse lymph node high endothelial cells.
    Miyasaka M
    International immunology 11.12 (1999 Dec): 1989-98.

    Heme oxygenase-1 expression enhances vascular endothelial resistance to complement-mediated injury through induction of decay-accelerating factor: a role for increased bilirubin and ferritin.
    Mason JC
    Blood 113.7 (2009 Feb 12): 1598-607.

    Biodistribution of (125)I-labeled anti-endoglin antibody using SPECT/CT imaging: Impact of in vivo deiodination on tumor accumulation in mice.
    Gallez B
    Nuclear medicine and biology 43.7 (2016 Jul): 415-23.

    PKCε-CREB-Nrf2 signalling induces HO-1 in the vascular endothelium and enhances resistance to inflammation and apoptosis.
    Mason JC
    Cardiovascular research 106.3 (2015 Jun 1): 509-19.

    Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin.
    Clegg DO
    Stem cells and development 19.8 (2010 Aug): 1231-40.

    (89)Zr-labeled anti-endoglin antibody-targeted gold nanoparticles for imaging cancer: implications for future cancer therapy.
    Gallez B
    Nanomedicine (London, England) 9.13 (2014 Sep): 1923-37.

    Stimulated shedding of vascular cell adhesion molecule 1 (VCAM-1) is mediated by tumor necrosis factor-alpha-converting enzyme (ADAM 17).
    Raines EW
    The Journal of biological chemistry 278.39 (2003 Sep 26): 37459-64.

    Decay-accelerating factor induction by tumour necrosis factor-alpha, through a phosphatidylinositol-3 kinase and protein kinase C-dependent pathway, protects murine vascular endothelial cells against complement deposition.
    Mason JC
    Immunology 110.2 (2003 Oct): 258-68.

    Isolation and analysis of large and small vessel endothelial cells.
    Yarwood H
    Methods in molecular medicine 135. (2007): 305-21.

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