MJ7/18
Catalog Fields
Clone ID/Product Name: MJ7/18
Available to For-Profits: Yes
Alternate Antibody Name: CD105
Gene Symbol: Eng
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2262267
Uniprot ID: Q63961
Entrez Gene ID: 13805
Clonality: Monoclonal
Immunogen: Inflamed mouse skin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: endoglin (CD105)
Epitope Location or Sequence: extracellular domain
Alternate Antigen Name:
Deposit Date: 8/4/1995
Antigen Molecular Weight: Predicted: 70 kDa; Apparent: 180 kDa (nonreduced) or 90 (reduced)
Depositor: Butcher, E.C. / Jutila, M.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine and Montana State University
Antigen Species: Rat
Depositor Notes: Pan endothelial marker with an extremely weak (minimal) reactivity with early hematopoetic cells (J. Spangrude, personal communication).
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: Endoglin is a major glycoprotein of vascular endothelium. MJ7/18 is commonly used for the isolation of murine endothelial cells.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MJ7/18 was deposited to the DSHB by Butcher, E.C. / Jutila, M. (DSHB Hybridoma Product MJ7/18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand.
Butcher EC
Gene 138.1-2 (1994 Jan 28): 201-6.