2H8

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$45.00 to $425.00
SKU: 2H8
View product citations for antibody 2H8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD31 (PECAM-1)
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Bogen, S.A.
Isotype: Armenian Hamster IgG
Antigen Sequence:
Host Species: Armenian hamster
Depositors Institution: Boston University, Pathology & Lab Medicine
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antibody has low mmunoreactive with endothelium, platelets, and leukocytes.
Antigen Molecular Weight: Predicted: 81Kda, Apparent: 120-130kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Pecam
Immunogen: D10.G4.1 mouse IL4-producing CD4+ T-cell
Alternate Gene Names: Pecam-1
Alternate Antibody Name: PECAM-1 CD_antigen: CD31
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q08481 
Immunogen Sequence:
Entrez Gene ID: 18613 
Additional Characterization:  
Antibody Registry ID: AB_2161039 
Additional Information:
Recommended Applications: FACS, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2H8 was deposited to the DSHB by Bogen, S.A. (DSHB Hybridoma Product 2H8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    IHC References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

    Integral role for lysyl oxidase-like-1 in conventional outflow tissue function and behavior.
    Stamer WD
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34.8 (2020 Aug): 10762-10777.

    Age-related focal loss of contractile vascular smooth muscle cells in retinal arterioles is accelerated by caveolin-1 deficiency.
    Elliott MH
    Neurobiology of aging 71. (2018 Nov): 1-12.

    IP References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    FB References

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

    FACS References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    All References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

    Integral role for lysyl oxidase-like-1 in conventional outflow tissue function and behavior.
    Stamer WD
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34.8 (2020 Aug): 10762-10777.

    Age-related focal loss of contractile vascular smooth muscle cells in retinal arterioles is accelerated by caveolin-1 deficiency.
    Elliott MH
    Neurobiology of aging 71. (2018 Nov): 1-12.

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