2H8

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$40.00
SKU: 2H8
View product citations for antibody 2H8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD31 (PECAM-1)
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Bogen, S.A.
Isotype: Armenian Hamster IgG
Antigen Sequence:
Host Species: Armenian hamster
Depositors Institution: Pathology & Lab Medicine, Boston University
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antibody has low mmunoreactive with endothelium, platelets, and leukocytes.
Antigen Molecular Weight: Predicted: 81Kda, Apparent: 120-130kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Pecam
Immunogen: D10.G4.1 mouse IL4-producing CD4+ T-cell
Alternate Gene Names: Pecam-1
Alternate Antibody Name: PECAM-1 CD_antigen: CD31
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q08481 
Immunogen Sequence:
Entrez Gene ID: 18613 
Additional Characterization:
Antibody Registry ID: AB_2161039 
Additional Information:
Recommended Applications: FACS, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2H8 was deposited to the DSHB by Bogen, S.A. (DSHB Hybridoma Product 2H8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    IHC References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

    IP References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    FB References

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

    FACS References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    All References

    Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Abbas AK
    The American journal of pathology 141.4 (1992 Oct): 843-54.

    Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    Muller WA
    The Journal of experimental medicine 179.3 (1994 Mar 1): 1059-64.

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