MECA-32
Catalog Fields
Antigen: plasmalemma vesicle-associated protein (PLVAP)
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG2a
Depositor: Butcher, E.C.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse
Depositors Institution: Stanford University School of Medicine
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 50-55 kDa (reducing conditions), 100-120 kDa (nonreducing)
Depositors Notes: Binds to murine endothelial cells. most or all EC except for capillaries in skeletal and cardiac muscle, and vessels in the CNS. The antigen is downregulated during CNS development in association with formation of the blood brain barrier, and is induced in negative vessels in muscle and CNS in association with inflammation.
Immunogen: Lymph node stroma.
Gene: Plvap
Alternate Antibody Name:
Alternate Gene Names: Pv1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q91VC4
Epitope Location or Sequence:
Entrez Gene ID: 84094
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_531797
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Marker of fenestrated vasculature
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MECA-32 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product MECA-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration.
Orosz CG
Transplantation 48.5 (1989 Nov): 874-7.