MECA-32

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SKU: MECA-32

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MECA-32
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Plvap
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID: AB_531797 
Uniprot ID: Q91VC4 
RRID:  
Entrez Gene ID: 84094 
Clonality: Monoclonal
Immunogen: Lymph node stroma.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: plasmalemma vesicle-associated protein (PLVAP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/4/1995
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 50-55 kDa (reducing conditions), 100-120 kDa (nonreducing)
Depositor: Butcher, E.C.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine
Antigen Species: Mouse
Depositor Notes: Binds to murine endothelial cells. most or all EC except for capillaries in skeletal and cardiac muscle, and vessels in the CNS. The antigen is downregulated during CNS development in association with formation of the blood brain barrier, and is induced in negative vessels in muscle and CNS in association with inflammation.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: Marker of fenestrated vasculature
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MECA-32 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product MECA-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    IHC References

    Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration.
    Orosz CG
    Transplantation 48.5 (1989 Nov): 874-7.

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    Cell adhesion molecules on vessels during inflammation in the mouse central nervous system.
    Butcher EC
    Journal of neuroimmunology 51.2 (1994 May): 199-208.

    Premature thymic involution is independent of structural plasticity of the thymic stroma.
    Liston A
    European journal of immunology 45.5 (2015 May): 1535-47.

    Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited.
    Kolesnick R
    Cancer research 70.20 (2010 Oct 15): 8179-86.

    Combination of a monoclonal anti-phosphatidylserine antibody with gemcitabine strongly inhibits the growth and metastasis of orthotopic pancreatic tumors in mice.
    Brekken RA
    International journal of cancer 118.10 (2006 May 15): 2639-43.

    Targeting acid sphingomyelinase with anti-angiogenic chemotherapy.
    Haimovitz-Friedman A
    Cellular signalling 29. (2017 Jan): 52-61.

    The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity.
    Gudjonsson JE
    JCI insight 4.8 (2019 Apr 18): .

    Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice.
    Blackshaw S
    Glia 68.10 (2020 Oct): 1987-2000.

    Macrophage-derived PDGF-B induces muscularization in murine and human pulmonary hypertension.
    Greif DM
    JCI insight 6.6 (2021 Mar 22): .

    IP References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    FFPE References

    Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited.
    Kolesnick R
    Cancer research 70.20 (2010 Oct 15): 8179-86.

    Targeting acid sphingomyelinase with anti-angiogenic chemotherapy.
    Haimovitz-Friedman A
    Cellular signalling 29. (2017 Jan): 52-61.

    All References

    Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration.
    Orosz CG
    Transplantation 48.5 (1989 Nov): 874-7.

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    Cell adhesion molecules on vessels during inflammation in the mouse central nervous system.
    Butcher EC
    Journal of neuroimmunology 51.2 (1994 May): 199-208.

    Premature thymic involution is independent of structural plasticity of the thymic stroma.
    Liston A
    European journal of immunology 45.5 (2015 May): 1535-47.

    Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited.
    Kolesnick R
    Cancer research 70.20 (2010 Oct 15): 8179-86.

    Combination of a monoclonal anti-phosphatidylserine antibody with gemcitabine strongly inhibits the growth and metastasis of orthotopic pancreatic tumors in mice.
    Brekken RA
    International journal of cancer 118.10 (2006 May 15): 2639-43.

    Targeting acid sphingomyelinase with anti-angiogenic chemotherapy.
    Haimovitz-Friedman A
    Cellular signalling 29. (2017 Jan): 52-61.

    The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity.
    Gudjonsson JE
    JCI insight 4.8 (2019 Apr 18): .

    Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice.
    Blackshaw S
    Glia 68.10 (2020 Oct): 1987-2000.

    Macrophage-derived PDGF-B induces muscularization in murine and human pulmonary hypertension.
    Greif DM
    JCI insight 6.6 (2021 Mar 22): .

    Changes in endothelial cell proliferation and vascular permeability after systemic lipopolysaccharide administration in the subfornical organ.
    Wanaka A
    Journal of neuroimmunology 298. (2016 Sep 15): 132-7.

    Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain.
    Miyata S
    Cell and tissue research 363.2 (2016 Feb): 497-511.

    Similarities and differences of human and experimental mouse lymphangiomas.
    Wilting J
    Developmental dynamics : an official publication of the American Association of Anatomists 236.10 (2007 Oct): 2952-61.

    Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain.
    Miyata S
    Cell and tissue research 359.3 (2015 Mar): 865-84.

    Pretreatment with anti-VEGF therapy may exacerbate inflammation in experimental acute colitis.
    Frischer JS
    Journal of pediatric surgery 47.2 (2012 Feb): 347-54.

    Tumor-derived intercellular adhesion molecule-1 mediates tumor-associated leukocyte infiltration in orthotopic pancreatic xenografts.
    Barnett CC Jr
    Experimental biology and medicine (Maywood, N.J.) 235.2 (2010 Feb): 263-70.

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