MECA-32

(0) No Reviews yet
$40.00
SKU: MECA-32
View product citations for antibody MECA-32 on CiteAb

In Stock

Available: 392

DSHB Data Sheet

Catalog Fields

Antigen: plasmalemma vesicle-associated protein (PLVAP)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Butcher, E.C.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Binds to murine endothelial cells. most or all EC except for capillaries in skeletal and cardiac muscle, and vessels in the CNS. The antigen is downregulated during CNS development in association with formation of the blood brain barrier, and is induced in negative vessels in muscle and CNS in association with inflammation.
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 50-55 kDa (reducing conditions), 100-120 kDa (nonreducing)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Plvap
Immunogen: Lymph node stroma.
Alternate Gene Names: Pv1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q91VC4 
Immunogen Sequence: Full length protein
Entrez Gene ID: 84094 
Additional Characterization:
Antibody Registry ID: AB_531797 
Additional Information: Marker of fenestrated vasculature
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MECA-32 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product MECA-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    IHC References
    IP References

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    FFPE References

    Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited.
    Kolesnick R
    Cancer research 70.20 (2010 Oct 15): 8179-86.

    Targeting acid sphingomyelinase with anti-angiogenic chemotherapy.
    Haimovitz-Friedman A
    Cellular signalling 29. (2017 Jan): 52-61.

    All References

    Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration.
    Orosz CG
    Transplantation 48.5 (1989 Nov): 874-7.

    Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Butcher EC
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 325-32.

    Cell adhesion molecules on vessels during inflammation in the mouse central nervous system.
    Butcher EC
    Journal of neuroimmunology 51.2 (1994 May): 199-208.

    Premature thymic involution is independent of structural plasticity of the thymic stroma.
    Liston A
    European journal of immunology 45.5 (2015 May): 1535-47.

    Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited.
    Kolesnick R
    Cancer research 70.20 (2010 Oct 15): 8179-86.

    Combination of a monoclonal anti-phosphatidylserine antibody with gemcitabine strongly inhibits the growth and metastasis of orthotopic pancreatic tumors in mice.
    Brekken RA
    International journal of cancer 118.10 (2006 May 15): 2639-43.

    Targeting acid sphingomyelinase with anti-angiogenic chemotherapy.
    Haimovitz-Friedman A
    Cellular signalling 29. (2017 Jan): 52-61.

    The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity.
    Gudjonsson JE
    JCI insight 4.8 (2019 Apr 18): .

    Changes in endothelial cell proliferation and vascular permeability after systemic lipopolysaccharide administration in the subfornical organ.
    Wanaka A
    Journal of neuroimmunology 298. (2016 Sep 15): 132-7.

    Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain.
    Miyata S
    Cell and tissue research 363.2 (2016 Feb): 497-511.

    Similarities and differences of human and experimental mouse lymphangiomas.
    Wilting J
    Developmental dynamics : an official publication of the American Association of Anatomists 236.10 (2007 Oct): 2952-61.

    Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain.
    Miyata S
    Cell and tissue research 359.3 (2015 Mar): 865-84.

    Pretreatment with anti-VEGF therapy may exacerbate inflammation in experimental acute colitis.
    Frischer JS
    Journal of pediatric surgery 47.2 (2012 Feb): 347-54.

    Tumor-derived intercellular adhesion molecule-1 mediates tumor-associated leukocyte infiltration in orthotopic pancreatic xenografts.
    Barnett CC Jr
    Experimental biology and medicine (Maywood, N.J.) 235.2 (2010 Feb): 263-70.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review