MT4

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$40.00
SKU: MT4
View product citations for antibody MT4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin
Hybridoma Cells Available: Yes
Antigen Species: newt
Depositor: Tassava, R.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Ohio State University
Positive Tested Species Reactivity: Axolotl, Newt
Depositors Notes:
Antigen Molecular Weight: Apparent: multiple isoforms between 225 and 260 kDa (reducing conditions; 500 kDa under nonreducing conditions)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FN
Immunogen: Crude early-bud and mid-bud blastema wound epithelium extract of newt
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q91400 
Immunogen Sequence: Full length protein
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528245 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MT4 was deposited to the DSHB by Tassava, R.A. (DSHB Hybridoma Product MT4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Examination of fibronectin distribution and its sources in the regenerating newt limb by immunocytochemistry and in situ hybridization.
    Tassava RA
    Developmental dynamics : an official publication of the American Association of Anatomists 202.2 (1995 Feb): 153-64.

    IF References

    Examination of fibronectin distribution and its sources in the regenerating newt limb by immunocytochemistry and in situ hybridization.
    Tassava RA
    Developmental dynamics : an official publication of the American Association of Anatomists 202.2 (1995 Feb): 153-64.

    Extracellular matrix protein turnover during salamander limb regeneration.
    Wei Y
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 4.1 (1996 Jan-Mar): 75-81.

    WB References

    Examination of fibronectin distribution and its sources in the regenerating newt limb by immunocytochemistry and in situ hybridization.
    Tassava RA
    Developmental dynamics : an official publication of the American Association of Anatomists 202.2 (1995 Feb): 153-64.

    All References

    Examination of fibronectin distribution and its sources in the regenerating newt limb by immunocytochemistry and in situ hybridization.
    Tassava RA
    Developmental dynamics : an official publication of the American Association of Anatomists 202.2 (1995 Feb): 153-64.

    Extracellular matrix protein turnover during salamander limb regeneration.
    Wei Y
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 4.1 (1996 Jan-Mar): 75-81.

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