8C8

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$40.00
SKU: 8C8
View product citations for antibody 8C8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (Xenopus)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus laevis
Depositor: Hausen, P. / Gawantka, V.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: MPI Tuebingen
Positive Tested Species Reactivity: Fish, Xenopus
Depositors Notes: 8C8 doesn't recognize the reduced protein. For immunohistochemistry, formaldehyde or methanol fixation work well.
Antigen Molecular Weight: Predicted: 86 kDa; Apparent: 100 kDa (precursor) and 115 kDa (mature protein) under non-reducing conditions and 125 kDa (precursor) and 135 kDa (mature protein) under reducing conditions
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: itgb1-a and itgb1-b
Immunogen: Protein fraction enriched in beta1-integrin precursor protein from XTC cells
Alternate Gene Names: CD29
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS I
Epitope Location or Sequence:
Uniprot ID: P12606 P12607 
Immunogen Sequence:
Entrez Gene ID: 397755 399443 
Additional Characterization:
Antibody Registry ID: AB_528309 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8C8 was deposited to the DSHB by Hausen, P. / Gawantka, V. (DSHB Hybridoma Product 8C8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
    Hausen P
    Development (Cambridge, England) 115.2 (1992 Jun): 595-605.

    Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus.
    Cozzi RR
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 150.3 (2008 Jul): 288-300.

    Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
    Nienhaus GU
    Nature communications 4. (2013): 2093.

    Polarized Wnt signaling regulates ectodermal cell fate in Xenopus.
    Niehrs C
    Developmental cell 29.2 (2014 Apr 28): 250-7.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    Cadherin-11 localizes to focal adhesions and promotes cell-substrate adhesion.
    Kashef J
    Nature communications 7. (2016 Mar 8): 10909.

    Different thresholds of Wnt-Frizzled 7 signaling coordinate proliferation, morphogenesis and fate of endoderm progenitor cells.
    Zorn AM
    Developmental biology 378.1 (2013 Jun 1): 1-12.

    Developmental role of plk4 in Xenopus laevis and Danio rerio: implications for Seckel Syndrome.
    Crawford M
    Biochemistry and cell biology = Biochimie et biologie cellulaire 93.4 (2015 Aug): 396-404.

    Active zones on motor nerve terminals contain alpha 3beta 1 integrin.
    DeSimone DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4912-21.

    Evidence for beta 1-integrins on both apical and basal surfaces of Xenopus retinal pigment epithelium.
    Defoe DM
    Experimental eye research 64.1 (1997 Jan): 73-84.

    WB References

    Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
    Hausen P
    Development (Cambridge, England) 115.2 (1992 Jun): 595-605.

    The classical progesterone receptor mediates Xenopus oocyte maturation through a nongenomic mechanism.
    Liu XJ
    Proceedings of the National Academy of Sciences of the United States of America 97.23 (2000 Nov 7): 12607-12.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    Evidence for a functional interaction between integrins and G protein-activated inward rectifier K+ channels.
    Lester HA
    The Journal of biological chemistry 273.52 (1998 Dec 25): 34696-702.

    Evidence for beta 1-integrins on both apical and basal surfaces of Xenopus retinal pigment epithelium.
    Defoe DM
    Experimental eye research 64.1 (1997 Jan): 73-84.

    IHC References
    IP References

    Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
    Hausen P
    Development (Cambridge, England) 115.2 (1992 Jun): 595-605.

    Active zones on motor nerve terminals contain alpha 3beta 1 integrin.
    DeSimone DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4912-21.

    Evidence for beta 1-integrins on both apical and basal surfaces of Xenopus retinal pigment epithelium.
    Defoe DM
    Experimental eye research 64.1 (1997 Jan): 73-84.

    All References

    The left-right asymmetry of liver lobation is generated by Pitx2c-mediated asymmetries in the hepatic diverticulum.
    Nascone-Yoder N
    Developmental biology 439.2 (2018 Jul 15): 80-91.

    Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
    Hausen P
    Development (Cambridge, England) 115.2 (1992 Jun): 595-605.

    Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus.
    Cozzi RR
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 150.3 (2008 Jul): 288-300.

    Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
    Nienhaus GU
    Nature communications 4. (2013): 2093.

    Polarized Wnt signaling regulates ectodermal cell fate in Xenopus.
    Niehrs C
    Developmental cell 29.2 (2014 Apr 28): 250-7.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    Cadherin-11 localizes to focal adhesions and promotes cell-substrate adhesion.
    Kashef J
    Nature communications 7. (2016 Mar 8): 10909.

    Different thresholds of Wnt-Frizzled 7 signaling coordinate proliferation, morphogenesis and fate of endoderm progenitor cells.
    Zorn AM
    Developmental biology 378.1 (2013 Jun 1): 1-12.

    Developmental role of plk4 in Xenopus laevis and Danio rerio: implications for Seckel Syndrome.
    Crawford M
    Biochemistry and cell biology = Biochimie et biologie cellulaire 93.4 (2015 Aug): 396-404.

    Active zones on motor nerve terminals contain alpha 3beta 1 integrin.
    DeSimone DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4912-21.

    Evidence for beta 1-integrins on both apical and basal surfaces of Xenopus retinal pigment epithelium.
    Defoe DM
    Experimental eye research 64.1 (1997 Jan): 73-84.

    The classical progesterone receptor mediates Xenopus oocyte maturation through a nongenomic mechanism.
    Liu XJ
    Proceedings of the National Academy of Sciences of the United States of America 97.23 (2000 Nov 7): 12607-12.

    Evidence for a functional interaction between integrins and G protein-activated inward rectifier K+ channels.
    Lester HA
    The Journal of biological chemistry 273.52 (1998 Dec 25): 34696-702.

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