8C8
Catalog Fields
Product Name/ID: 8C8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: itgb1-a and itgb1-b
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528309
Uniprot ID: P12606 P12607
Entrez Gene ID: 397755 399443
Clonality: Monoclonal
Immunogen: Protein fraction enriched in beta1-integrin precursor protein from XTC cells
Clone:
Immunogen Sequence:
Myeloma Strain: NS I
Epitope Mapped: No
Antigen Name: integrin beta-1 (Xenopus)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/20/1994
Antigen Molecular Weight: Predicted: 86 kDa; Apparent: 100 kDa (precursor) and 115 kDa (mature protein) under non-reducing conditions and 125 kDa (precursor) and 135 kDa (mature protein) under reducing conditions
Depositor: Hausen, P. / Gawantka, V.
Antigen Sequence:
Depositor Institution: MPI Tuebingen
Antigen Species: Xenopus laevis
Depositor Notes: 8C8 doesn't recognize the reduced protein. For immunohistochemistry, formaldehyde or methanol fixation work well.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Fish, Xenopus
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8C8 was deposited to the DSHB by Hausen, P. / Gawantka, V. (DSHB Hybridoma Product 8C8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
Hausen P
Development (Cambridge, England) 115.2 (1992 Jun): 595-605.