MW7

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$40.00
SKU: MW7
View product citations for antibody MW7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: huntingtin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Patterson, P.H.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes:
Antigen Molecular Weight: Apparent: 348 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: HTT
Immunogen: Recombinant Hd exon-1 67Q aggregate
Alternate Gene Names: HD, IT15
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: N-terminal, polyP
Uniprot ID: P42858 
Immunogen Sequence: Partial protein
Entrez Gene ID: 3064 
Additional Characterization:  
Antibody Registry ID: AB_528296 
Additional Information: Western blot: in Human, weak binding to high molecular weight Htt, and strong binding to two smaller protein bands (doesn't differentiate between HD and non-HD); in mice, preferential strong binding to expanded polyQ domain. ICC: perinuclear or nuclear membranes in WT and expanded polyQ domain mutant; stains aggregates as inclusions in the nuclei of expanded polyQ domain mutant.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW7 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    Mutant huntingtin is present in neuronal grafts in Huntington disease patients.
    Freeman TB
    Annals of neurology 76.1 (2014 Jul): 31-42.

    A compact beta model of huntingtin toxicity.
    Poirier MA
    The Journal of biological chemistry 286.10 (2011 Mar 11): 8188-96.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Human-to-mouse prion-like propagation of mutant huntingtin protein.
    Song J
    Acta neuropathologica 132.4 (2016 Oct): 577-92.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    WB References

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    IHC References

    Mutant huntingtin is present in neuronal grafts in Huntington disease patients.
    Freeman TB
    Annals of neurology 76.1 (2014 Jul): 31-42.

    Human mutant huntingtin disrupts vocal learning in transgenic songbirds.
    Lee R
    Nature neuroscience 18.11 (2015 Nov): 1617-22.

    ELISA References
    FB References
    Epitope Map References
    All References

    Mutant huntingtin is present in neuronal grafts in Huntington disease patients.
    Freeman TB
    Annals of neurology 76.1 (2014 Jul): 31-42.

    Human mutant huntingtin disrupts vocal learning in transgenic songbirds.
    Lee R
    Nature neuroscience 18.11 (2015 Nov): 1617-22.

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    A compact beta model of huntingtin toxicity.
    Poirier MA
    The Journal of biological chemistry 286.10 (2011 Mar 11): 8188-96.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Human-to-mouse prion-like propagation of mutant huntingtin protein.
    Song J
    Acta neuropathologica 132.4 (2016 Oct): 577-92.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity.
    Patterson PH
    Proceedings of the National Academy of Sciences of the United States of America 99.2 (2002 Jan 22): 1002-7.

    In vitro and in vivo aggregation of a fragment of huntingtin protein directly causes free radical production.
    Wyttenbach A
    The Journal of biological chemistry 286.52 (2011 Dec 30): 44512-20.

    Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core.
    van der Wel PCA
    Nature communications 8. (2017 May 24): 15462.

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