MW1
Catalog Fields
Clone ID/Product Name: MW1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: HTT
Ab Isotype: MIgG2b
Gene Name:
Entrez Gene ID: 3064
Clonality: Monoclonal
Immunogen: Recombinant DRPLA-19Q/GST fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: HL-1
Epitope Mapped: Yes
Antigen Name: huntingtin (specific for expanded polyQ by western blot)
Epitope Location or Sequence: N-terminal, polyQ
Alternate Antigen Name:
Deposit Date: 1/23/2003
Antigen Molecular Weight: Apparent: 348 kDa
Depositor: Patterson, P.H.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Human
Depositor Notes: MW1 is specific for the elongated polyQ stretch through which mHtt aggregates. The epitope is hidden in aggregates. This antibody is also known as anti-polyQ.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Appears to recognize an altered conformation of the polyQ domain generated as the polyQ domain of Htt increases in length. Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW1 to assay soluble huntingtin. PMID: 22365609 23966247 23626768 23100324 22984513. RRID:AB_528290
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW1 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
Patterson PH
Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.