Antibody Name: MW1
Antigen:huntingtin (specific for expanded polyQ by western blot)
Western blot of polyglutamine expression in ISPN lines using DSHB anti-polyQ antibody MW1. PMID: 34296279, Fig. 4D.
Catalog Fields
Identity
Clone ID / Antibody Name: MW1
Alternate Antibody Name: —
Antigen Name: huntingtin (specific for expanded polyQ by western blot)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: HTT
Entrez Gene ID: 3064
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Apparent: 348 kDa
Uniprot ID: P42858
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG2b
Myeloma Strain: HL-1
Antibody Registry / RRID: AB_528290
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Recombinant DRPLA-19Q/GST fusion protein
Immunogen Sequence: Partial protein
Epitope Mapped: Yes
Epitope Location / Seq: N-terminal, polyQ
Clone: —
Additional Info: Appears to recognize an altered conformation of the polyQ domain generated as the polyQ domain of Htt increases in length. Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW1 to assay soluble huntingtin. PMID: 22365609 23966247 23626768 23100324 22984513. RRID:AB_528290
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Human
Predicted Reactivity: —
Depositor
Depositor Name: P.H. Patterson
Institution: California Institute of Technology
Deposit Date: January 23, 2003
Depositor Notes: MW1 is specific for the elongated polyQ stretch through which mHtt aggregates. The epitope is hidden in aggregates. This antibody is also known as anti-polyQ.
Recommended Applications
ELISA
FACS
Function Blocking
IF
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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