MW1

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SKU: MW1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MW1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: HTT
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_528290 
Uniprot ID: P42858 
RRID:  
Entrez Gene ID: 3064 
Clonality: Monoclonal
Immunogen: Recombinant DRPLA-19Q/GST fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: HL-1
Epitope Mapped: Yes
Antigen Name: huntingtin (specific for expanded polyQ by western blot)
Epitope Location or Sequence: N-terminal, polyQ
Alternate Antigen Name:
Deposit Date: 1/23/2003
Antigen Molecular Weight: Apparent: 348 kDa
Depositor: Patterson, P.H.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Human
Depositor Notes: MW1 is specific for the elongated polyQ stretch through which mHtt aggregates. The epitope is hidden in aggregates. This antibody is also known as anti-polyQ.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Appears to recognize an altered conformation of the polyQ domain generated as the polyQ domain of Htt increases in length. Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW1 to assay soluble huntingtin. PMID: 22365609 23966247 23626768 23100324 22984513. RRID:AB_528290
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW1 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    Mimicking proteasomal release of polyglutamine peptides initiates aggregation and toxicity.
    Reits E
    Journal of cell science 122.Pt 18 (2009 Sep 15): 3262-71.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    WB References
    IP References
    ELISA References
    FB References
    FACS References
    Epitope Map References
    All References

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    Mimicking proteasomal release of polyglutamine peptides initiates aggregation and toxicity.
    Reits E
    Journal of cell science 122.Pt 18 (2009 Sep 15): 3262-71.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    Mutant huntingtin is present in neuronal grafts in Huntington disease patients.
    Freeman TB
    Annals of neurology 76.1 (2014 Jul): 31-42.

    Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease.
    Bates GP
    The Journal of biological chemistry 285.12 (2010 Mar 19): 8808-23.

    Caspase-6 does not contribute to the proteolysis of mutant huntingtin in the HdhQ150 knock-in mouse model of Huntington's disease.
    Bates G
    PLoS currents 4. (2012 Jul 16): e4fd085bfc9973.

    Mitochondrial membranes modify mutant huntingtin aggregation.
    Legleiter J
    Biochimica et biophysica acta. Biomembranes 1863.10 (2021 Oct 1): 183663.

    Ultrasensitive measurement of huntingtin protein in cerebrospinal fluid demonstrates increase with Huntington disease stage and decrease following brain huntingtin suppression.
    Hayden MR
    Scientific reports 5. (2015 Jul 15): 12166.

    Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity.
    Patterson PH
    Proceedings of the National Academy of Sciences of the United States of America 99.2 (2002 Jan 22): 1002-7.

    Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core.
    van der Wel PCA
    Nature communications 8. (2017 May 24): 15462.

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