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Available: 49

DSHB Data Sheet

Catalog Fields

Antigen: Huntingtin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Patterson, P.H.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: The MW8 exclusively stains aggregates as inclusions in both nuclei and in the neuropil. This antigen is also known as huntington disease protein.
Antigen Molecular Weight: 347.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: HTT
Immunogen: Hd exon-1 67Q aggregate
Alternate Gene Names: HD, IT15
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: AEEPLHRPK/ a.a. 83-90
Uniprot ID: P42858 
Immunogen Sequence: HDx-67Q
Entrez Gene ID: 3064 
Additional Characterization:
Antibody Registry ID: AB_528297 
Additional Information: RRID:AB_528297; Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW8 to assay huntingtin aggregates. PMID: 22365609 22984513
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW8 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References
    Epitope Map References
    All References

    New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
    Patterson PH
    Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.

    Deletion of the huntingtin polyglutamine stretch enhances neuronal autophagy and longevity in mice.
    Zeitlin SO
    PLoS genetics 6.2 (2010 Feb 5): e1000838.

    A series of N-terminal epitope tagged Hdh knock-in alleles expressing normal and mutant huntingtin: their application to understanding the effect of increasing the length of normal Huntingtin's polyglutamine stretch on CAG140 mouse model pathogenesis.
    Zeitlin SO
    Molecular brain 5. (2012 Aug 14): 28.

    Methylene blue modulates huntingtin aggregation intermediates and is protective in Huntington's disease models.
    Thompson LM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.32 (2012 Aug 8): 11109-19.

    Human mutant huntingtin disrupts vocal learning in transgenic songbirds.
    Lee R
    Nature neuroscience 18.11 (2015 Nov): 1617-22.

    Comparison of mHTT Antibodies in Huntington's Disease Mouse Models Reveal Specific Binding Profiles and Steady-State Ubiquitin Levels with Disease Development.
    Brooks SP
    PloS one 11.5 (2016): e0155834.

    Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity.
    Patterson PH
    Proceedings of the National Academy of Sciences of the United States of America 99.2 (2002 Jan 22): 1002-7.

    Mutant huntingtin gene-dose impacts on aggregate deposition, DARPP32 expression and neuroinflammation in HdhQ150 mice.
    Schmid P
    PloS one 8.9 (2013): e75108.

    Fragments of HdhQ150 mutant huntingtin form a soluble oligomer pool that declines with aggregate deposition upon aging.
    Lotz GP
    PloS one 7.9 (2012): e44457.

    Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment.
    Muchowski PJ
    The Journal of biological chemistry 284.32 (2009 Aug 7): 21647-58.

    Differential morphology and composition of inclusions in the R6/2 mouse and PC12 cell models of Huntington's disease.
    Morton AJ
    Histochemistry and cell biology 127.5 (2007 May): 473-84.

    Identifying polyglutamine protein species in situ that best predict neurodegeneration.
    Finkbeiner S
    Nature chemical biology 7.12 (2011 Oct 30): 925-34.

    Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease.
    Bates GP
    The Journal of biological chemistry 285.12 (2010 Mar 19): 8808-23.

    TR-FRET-based duplex immunoassay reveals an inverse correlation of soluble and aggregated mutant huntingtin in huntington's disease.
    Weiss A
    Chemistry and biology 19.2 (2012 Feb 24): 264-75.

    Native mutant huntingtin in human brain: evidence for prevalence of full-length monomer.
    DiFiglia M
    The Journal of biological chemistry 287.16 (2012 Apr 13): 13487-99.

    Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core.
    van der Wel PCA
    Nature communications 8. (2017 May 24): 15462.

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