P2A4
Catalog Fields
Clone ID/Product Name: P2A4
Available to For-Profits: Yes
Alternate Antibody Name: CD54
Gene Symbol: ICAM1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 3383
Clonality: Monoclonal
Immunogen: IL-1beta activated human umbilical vein endothelial cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Fox NY
Epitope Mapped: Yes
Antigen Name: intercellular adhesion molecule 1
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 8/4/1993
Antigen Molecular Weight: Predicted: 55 kDa
Depositor: Wayner, E.A. / Vercellotti, G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: human
Depositor Notes: inhibits binding of ICAM-1. Doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2A4 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
Toxoplasma gondii-induced immune suppression by human peripheral blood monocytes: role of gamma interferon.
Kasper LH
Infection and immunity 64.4 (1996 Apr): 1181-9.