P2A4

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$40.00
SKU: P2A4
View product citations for antibody P2A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: intercellular adhesion molecule 1
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Wayner, E.A. / Vercellotti, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: inhibits binding of ICAM-1. Doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 55 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ICAM1
Immunogen: IL-1beta activated human umbilical vein endothelial cells
Alternate Gene Names: BB2, CD54, P3.58
Alternate Antibody Name: CD54
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox NY
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P05362 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3383 
Additional Characterization:  
Antibody Registry ID: AB_528298 
Additional Information:
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2A4 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Circulating CD34+ cells in cord blood and mobilized blood have a different profile of adhesion molecules than bone marrow CD34+ cells.
    De Waele M
    European journal of haematology 60.3 (1998 Mar): 153-60.

    Endothelial PI 3-kinase activity regulates lymphocyte diapedesis.
    Murray AG
    American journal of physiology. Heart and circulatory physiology 293.6 (2007 Dec): H3608-16.

    Complement component C5a activates ICAM-1 expression on human choroidal endothelial cells.
    Mullins RF
    Investigative ophthalmology & visual science 51.10 (2010 Oct): 5336-42.

    IHC References
    IP References

    Hypoxia enhances induction of endothelial ICAM-1: role for metabolic acidosis and proteasomes.
    Colgan SP
    The American journal of physiology 273.5 (1997 Nov): C1571-80.

    ELISA References

    Hypoxia enhances induction of endothelial ICAM-1: role for metabolic acidosis and proteasomes.
    Colgan SP
    The American journal of physiology 273.5 (1997 Nov): C1571-80.

    Endothelial cell injury in cardiac surgery: salicylate may be protective by reducing expression of endothelial adhesion molecules.
    Turina M
    European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 13.3 (1998 Mar): 293-7.

    FACS References

    Toxoplasma gondii-induced immune suppression by human peripheral blood monocytes: role of gamma interferon.
    Kasper LH
    Infection and immunity 64.4 (1996 Apr): 1181-9.

    Endothelial PI 3-kinase activity regulates lymphocyte diapedesis.
    Murray AG
    American journal of physiology. Heart and circulatory physiology 293.6 (2007 Dec): H3608-16.

    Different expression of adhesion molecules on myeloid and B-lymphoid CD34+ progenitors in normal bone marrow.
    Van Riet I
    European journal of haematology 59.5 (1997 Nov): 277-86.

    Adhesion molecules expressed on homing lymphocytes in model intestinal epithelia.
    Madara JL
    Gastroenterology 118.2 (2000 Feb): 289-98.

    All References

    Macular and peripheral distribution of ICAM-1 in the human choriocapillaris and retina.
    Kuehn MH
    Molecular vision 12. (2006 Mar 30): 224-35.

    Circulating CD34+ cells in cord blood and mobilized blood have a different profile of adhesion molecules than bone marrow CD34+ cells.
    De Waele M
    European journal of haematology 60.3 (1998 Mar): 153-60.

    Endothelial PI 3-kinase activity regulates lymphocyte diapedesis.
    Murray AG
    American journal of physiology. Heart and circulatory physiology 293.6 (2007 Dec): H3608-16.

    Complement component C5a activates ICAM-1 expression on human choroidal endothelial cells.
    Mullins RF
    Investigative ophthalmology & visual science 51.10 (2010 Oct): 5336-42.

    Hypoxia enhances induction of endothelial ICAM-1: role for metabolic acidosis and proteasomes.
    Colgan SP
    The American journal of physiology 273.5 (1997 Nov): C1571-80.

    Endothelial cell injury in cardiac surgery: salicylate may be protective by reducing expression of endothelial adhesion molecules.
    Turina M
    European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 13.3 (1998 Mar): 293-7.

    Toxoplasma gondii-induced immune suppression by human peripheral blood monocytes: role of gamma interferon.
    Kasper LH
    Infection and immunity 64.4 (1996 Apr): 1181-9.

    Different expression of adhesion molecules on myeloid and B-lymphoid CD34+ progenitors in normal bone marrow.
    Van Riet I
    European journal of haematology 59.5 (1997 Nov): 277-86.

    Adhesion molecules expressed on homing lymphocytes in model intestinal epithelia.
    Madara JL
    Gastroenterology 118.2 (2000 Feb): 289-98.

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