P2B1
Catalog Fields
Antigen: PECAM1 (CD31)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Vercellotti, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Canine, Human, Mouse, Rat
Depositors Notes: This antibody doesn't appear to work for western blotting.
Antigen Molecular Weight: Multiple isoforms; Predicted: 76 to 80 kDa; Apparent: 120 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: PECAM1
Immunogen: IL-1beta activated human umbilical vein endothelial cells.
Alternate Gene Names: CD31, EndoCAM, GPIIA', PECA1
Alternate Antibody Name: CD31
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox NY
Epitope Location or Sequence: extracellular
Uniprot ID: P16284
Immunogen Sequence: Full length protein
Entrez Gene ID: 5175
Additional Characterization:
Antibody Registry ID: AB_528430
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2B1 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P2B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin.
Cheresh DA
The Journal of clinical investigation 96.4 (1995 Oct): 1815-22.