P2B1

(0) No Reviews yet
$40.00
SKU: P2B1
View product citations for antibody P2B1 on CiteAb

In Stock

Available: 49

DSHB Data Sheet

Catalog Fields

Antigen: PECAM1 (CD31)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Vercellotti, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Canine, Human, Mouse, Rat
Depositors Notes: This antibody doesn't appear to work for western blotting.
Antigen Molecular Weight: Multiple isoforms; Predicted: 76 to 80 kDa; Apparent: 120 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: PECAM1
Immunogen: IL-1beta activated human umbilical vein endothelial cells.
Alternate Gene Names: CD31, EndoCAM, GPIIA', PECA1
Alternate Antibody Name: CD31
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox NY
Epitope Location or Sequence: extracellular
Uniprot ID: P16284 
Immunogen Sequence: Full length protein
Entrez Gene ID: 5175 
Additional Characterization:
Antibody Registry ID: AB_528430 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2B1 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P2B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • IHC References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin.
    Cheresh DA
    The Journal of clinical investigation 96.4 (1995 Oct): 1815-22.

    IF References

    Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin.
    Cheresh DA
    The Journal of clinical investigation 96.4 (1995 Oct): 1815-22.

    Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.
    Acott TS
    PloS one 10.3 (2015): e0122483.

    Alteration of cardiac progenitor cell potency in GRMD dogs.
    Sampaolesi M
    Cell transplantation 21.9 (2012): 1945-67.

    The creation of an in vitro adipose tissue that contains a vascular-adipocyte complex.
    Caplan AI
    Biomaterials 32.36 (2011 Dec): 9667-76.

    Platelet-endothelial cell adhesion molecule-1 (CD31) redistributes from the endothelial junction and is not required for the transendothelial migration of melanoma cells.
    Siu CH
    Clinical and experimental metastasis 18.6 (2000): 527-32.

    Molecular cloning, functional expression, and tissue distribution of a novel human gap junction-forming protein, connexin-31.9. Interaction with zona occludens protein-1.
    Kumar NM
    The Journal of biological chemistry 277.41 (2002 Oct 11): 38272-83.

    Suppressing iron oxide nanoparticle toxicity by vascular targeted antioxidant polymer nanoparticles.
    Dziubla TD
    Biomaterials 34.37 (2013 Dec): 9615-22.

    Microfluidic Tumor-Vascular Model to Study Breast Cancer Cell Invasion and Intravasation.
    Nikkhah M
    Advanced healthcare materials 7.9 (2018 May): e1701257.

    Microfluidic Tumor-Vascular Model to Study Breast Cancer Cell Invasion and Intravasation.
    Nikkhah M
    Advanced healthcare materials 7.9 (2018 May): e1701257.

    IHC References

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    FB References
    Epitope Map References
    All References

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin.
    Cheresh DA
    The Journal of clinical investigation 96.4 (1995 Oct): 1815-22.

    Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.
    Acott TS
    PloS one 10.3 (2015): e0122483.

    Alteration of cardiac progenitor cell potency in GRMD dogs.
    Sampaolesi M
    Cell transplantation 21.9 (2012): 1945-67.

    The creation of an in vitro adipose tissue that contains a vascular-adipocyte complex.
    Caplan AI
    Biomaterials 32.36 (2011 Dec): 9667-76.

    Platelet-endothelial cell adhesion molecule-1 (CD31) redistributes from the endothelial junction and is not required for the transendothelial migration of melanoma cells.
    Siu CH
    Clinical and experimental metastasis 18.6 (2000): 527-32.

    Molecular cloning, functional expression, and tissue distribution of a novel human gap junction-forming protein, connexin-31.9. Interaction with zona occludens protein-1.
    Kumar NM
    The Journal of biological chemistry 277.41 (2002 Oct 11): 38272-83.

    Suppressing iron oxide nanoparticle toxicity by vascular targeted antioxidant polymer nanoparticles.
    Dziubla TD
    Biomaterials 34.37 (2013 Dec): 9615-22.

    Microfluidic Tumor-Vascular Model to Study Breast Cancer Cell Invasion and Intravasation.
    Nikkhah M
    Advanced healthcare materials 7.9 (2018 May): e1701257.

    Monoclonal antibody to CD31 (PECAM-1) inhibits tubular regeneration after ischemia reperfusion injury in the rat.
    Toubeau G
    Nephron. Experimental nephrology 118.3 (2011): e60-8.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review