P5G10

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$40.00
SKU: P5G10
View product citations for antibody P5G10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-6, human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits adhesion of PC3 (prostate cancer line) cells to laminin-1.
Antigen Molecular Weight: Multiple isoforms; Predicted: 107 to 126 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA6
Immunogen: PC3 (prostate cancer line) cells
Alternate Gene Names: CD49f, VLA-6
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: FOX?NY
Epitope Location or Sequence: extracellular
Uniprot ID: P23229 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3655 
Additional Characterization:  
Antibody Registry ID: AB_2619593 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5G10 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    IF References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Epitope Map References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    All References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

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