P5G10

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SKU: P5G10

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P5G10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ITGA6
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619593 
Uniprot ID: P23229 
RRID:  
Entrez Gene ID: 3655 
Clonality: Monoclonal
Immunogen: PC3 (prostate cancer line) cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: FOX?NY
Epitope Mapped: Yes
Antigen Name: integrin alpha-6, human
Epitope Location or Sequence: extracellular
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Multiple isoforms; Predicted: 107 to 126 kDa
Depositor: Wayner, E.A.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Inhibits adhesion of PC3 (prostate cancer line) cells to laminin-1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5G10 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    IF References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Epitope Map References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    All References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

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