P5G10

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$40.00
SKU: P5G10
View product citations for antibody P5G10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-6, human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits adhesion of PC3 (prostate cancer line) cells to laminin-1.
Antigen Molecular Weight: Multiple isoforms; Predicted: 107 to 126 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGA6
Immunogen: PC3 (prostate cancer line) cells
Alternate Gene Names: CD49f, VLA-6
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: FOX?NY
Epitope Location or Sequence: extracellular
Uniprot ID: P23229 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3655 
Additional Characterization:
Antibody Registry ID: AB_2619593 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5G10 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    IF References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Epitope Map References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    All References

    Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
    Cooper JA
    Journal of cell science 127.Pt 3 (2014 Feb 1): 509-20.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

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