P1B5
Catalog Fields
Clone ID/Product Name: P1B5
Available to For-Profits: Yes
Alternate Antibody Name: CD49c
Gene Symbol: ITGA3
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2619595
Uniprot ID: P26006
Entrez Gene ID: 3675
Clonality: Monoclonal
Immunogen: HT1080 human fibrosarcoma cell line
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin alpha-3 (CD49c)
Epitope Location or Sequence: NITVKN (75-80)
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: 119 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: P1B5 inhibits cell adhesion to collagen and fibronectin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2619595
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1B5 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
Kunicki TJ
The Journal of cell biology 107.5 (1988 Nov): 1881-91.