P1B5

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$40.00
SKU: P1B5
View product citations for antibody P1B5 on CiteAb

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Available: 101

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-3 (CD49c)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: P1B5 inhibits cell adhesion to collagen and fibronectin.
Antigen Molecular Weight: 119 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA3
Immunogen: HT1080 human fibrosarcoma cell line
Alternate Gene Names: CD49c, GAP-B3, MSK18, VCA-2, VLA3a
Alternate Antibody Name: CD49c
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: NITVKN (75-80)
Uniprot ID: P26006 
Immunogen Sequence:
Entrez Gene ID: 3675 
Additional Characterization:  
Antibody Registry ID: AB_2619595 
Additional Information: RRID:AB_2619595
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1B5 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References

    In PC3 prostate cancer cells ephrin receptors crosstalk to β1-integrins to strengthen adhesion to collagen type I.
    Helenius J
    Scientific reports 5. (2015 Feb 3): 8206.

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Integrin Ligation Results in Nephrin Tyrosine Phosphorylation In Vitro.
    Garg P
    PloS one 11.2 (2016): e0148906.

    A computer-assisted 3D model for analyzing the aggregation of tumorigenic cells reveals specialized behaviors and unique cell types that facilitate aggregate coalescence.
    Soll DR
    PloS one 10.3 (2015): e0118628.

    Quantitative Motion Analysis in Two and Three Dimensions.
    Soll DR
    Methods in molecular biology (Clifton, N.J.) 1365. (2016): 265-92.

    Integrin expression and function on human osteoblast-like cells.
    Simmons PJ
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1189-97.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FACS References
    Epitope Map References
    All References

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    In PC3 prostate cancer cells ephrin receptors crosstalk to β1-integrins to strengthen adhesion to collagen type I.
    Helenius J
    Scientific reports 5. (2015 Feb 3): 8206.

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Integrin Ligation Results in Nephrin Tyrosine Phosphorylation In Vitro.
    Garg P
    PloS one 11.2 (2016): e0148906.

    A computer-assisted 3D model for analyzing the aggregation of tumorigenic cells reveals specialized behaviors and unique cell types that facilitate aggregate coalescence.
    Soll DR
    PloS one 10.3 (2015): e0118628.

    Quantitative Motion Analysis in Two and Three Dimensions.
    Soll DR
    Methods in molecular biology (Clifton, N.J.) 1365. (2016): 265-92.

    Integrin expression and function on human osteoblast-like cells.
    Simmons PJ
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1189-97.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    Alpha 3 beta 1 adhesion to laminin-5 and invasin: critical and differential role of integrin residues clustered at the boundary between alpha 3 N-terminal repeats 2 and 3.
    Takada Y
    Biochemistry 38.43 (1999 Oct 26): 14424-31.

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