SUMO1 76-86

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SKU: SUMO1 76-86

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: SUMO1 76-86
Available to For-Profits: Yes
Alternate Antibody Name: 76-86
Gene Symbol: SUMO1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2618115 
Uniprot ID: P63165 
Entrez Gene ID: 7341 
Clonality: Monoclonal
Immunogen: SUMO-1 peptide (CIADNHTPKLELGMEEEDVIEVYQEQT) coupled to carrier protein
Immunogen Sequence: Peptide
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: SUMO1, aa 76-86
Epitope Location or Sequence: aa 76-86 TPKELGMEEED
Alternate Antigen Name:
Deposit Date: 8/20/2013
Antigen Molecular Weight: 11 kDa
Depositor: Melchior, F.
Antigen Sequence:
Depositor Institution: University of Heidelberg, ZMBH
Antigen Species: Human
Depositor Notes: This antibody recognizes SUMO1 and SUMO1 conjugates in mammalian cells, and likely in many other vertebrates (shared epitope).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Mouse, Xenopus
Additional Information: This antibody will recognize both SUMO-1 isoforms.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO1 76-86 was deposited to the DSHB by Melchior, F. (DSHB Hybridoma Product SUMO1 76-86)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Detecting endogenous SUMO targets in mammalian cells and tissues.
    Melchior F
    Nature structural & molecular biology 20.4 (2013 Apr): 525-31.

    WB References
    IP References
    Epitope Map References

    Detecting endogenous SUMO targets in mammalian cells and tissues.
    Melchior F
    Nature structural & molecular biology 20.4 (2013 Apr): 525-31.

    All References

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