SUMO1 76-86

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SKU: SUMO1 76-86
View product citations for antibody SUMO1 76-86 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SUMO1, aa 76-86
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Melchior, F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: ZMBH, University of Heidelberg
Positive Tested Species Reactivity: Chicken, Human, Mouse, Xenopus
Depositors Notes: This antibody recognizes SUMO1 and SUMO1 conjugates in mammalian cells, and likely in many other vertebrates (shared epitope).
Antigen Molecular Weight: 11 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SUMO1
Immunogen: SUMO-1 peptide (CIADNHTPKLELGMEEEDVIEVYQEQT) coupled to carrier protein
Alternate Gene Names: SMT3C, SMT3H3, UBL1, PIC1, DAP1, GMP1, OFC10, SENP2
Alternate Antibody Name: 76-86
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: aa 76-86 TPKELGMEEED
Uniprot ID: P63165 
Immunogen Sequence: Peptide
Entrez Gene ID: 7341 
Additional Characterization:  
Antibody Registry ID: AB_2618115 
Additional Information: This antibody will recognize both SUMO-1 isoforms.
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO1 76-86 was deposited to the DSHB by Melchior, F. (DSHB Hybridoma Product SUMO1 76-86)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Detecting endogenous SUMO targets in mammalian cells and tissues.
    Melchior F
    Nature structural & molecular biology 20.4 (2013 Apr): 525-31.

    WB References
    IP References
    Epitope Map References

    Detecting endogenous SUMO targets in mammalian cells and tissues.
    Melchior F
    Nature structural & molecular biology 20.4 (2013 Apr): 525-31.

    All References

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