SUMO-1 21C7
Catalog Fields
Antigen: SUMO1, aa 57-67
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Matunis, M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat, Xenopus, Zebrafish
Depositors Institution: Johns Hopkins University School of Medicine
Antigen Molecular Weight: 11 kDa
Depositors Notes: This antibody recognizes SUMO1 and SUMO1 conjugates in all vertebrate cells tested; does not cross-react with SUMO2 or SUMO3. 21C7 doesn't recognize C. elegans SUMO1.
Immunogen: Recombinant SUMO-1 (GenBank accession number AAB40390) protein with N-terminal 6xHis tag
Gene: SUMO1
Alternate Antibody Name: 21C7
Alternate Gene Names: SMT3C, SMT3H3, UBL1, PIC1, DAP1, GMP1, OFC10, SENP2
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Uniprot ID: P63165
Epitope Location or Sequence: aa 57-67 VPMNSLRFLFE
Entrez Gene ID: 7341
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2198257
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: This antibody will recognize both SUMO-1 isoforms.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO-1 21C7 was deposited to the DSHB by Matunis, M. (DSHB Hybridoma Product SUMO-1 21C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
Blobel G
The Journal of cell biology 135.6 Pt 1 (1996 Dec): 1457-70.