SUMO-2 8A2
Catalog Fields
Antigen: SUMO2 (SUMO-2/3)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Matunis, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Chicken, Drosophila melanogaster, Human, Mouse, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes SUMO2 and SUMO3 and their conjugates in all vertebrate cells tested; does not cross-react with SUMO1 .
Predicted Species Reactivity:
Gene: SUMO2
Immunogen: Recombinant SUMO-2 (GenBank accession number P61956) protein
Alternate Gene Names: SMT3B, SMT3H2
Alternate Antibody Name: 8A2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: aa 57-66, IRFRFDGQPI
Uniprot ID: P61956
Immunogen Sequence: Full length protein
Entrez Gene ID: 6613
Antibody Registry ID: AB_2198421
Additional Information: This antibody will not recognize isoform 2 of SUMO-2 missing aa 51-74, but will recognize both SUMO-3 isoforms.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO-2 8A2 was deposited to the DSHB by Matunis, M. (DSHB Hybridoma Product SUMO-2 8A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis.
Matunis MJ
Molecular cell 29.6 (2008 Mar 28): 729-41.