XAP-1 (Clone 3D2)
Catalog Fields
Antigen: photoreceptors (rods and cones)
Hybridoma Cells Available: No
Antigen Species: Xenopus
Depositor: Sakaguchi, D.S. / Harris, W.A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Axolotl, Cat, Chick, Frog, Hamster, Opossum, Rat, Salamander, Squirrel, Turtle
Depositors Notes: Cell binding note: appears membrane associated. Immunohistology note: labels photoreceptors in vertebrate retina. IHC note: yes, paraformaldehyde fixed tissue. labels photoreceptors in the retina also labels skin in Xenopus embryos and larvae.
Predicted Species Reactivity:
Gene:
Immunogen: immunosuppressed against Xenopus embryos, immunized with Xenopus tadpole optic nerves and retinae
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Antibody Registry ID: AB_531883
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
XAP-1 (Clone 3D2) was deposited to the DSHB by Sakaguchi, D.S. / Harris, W.A. (DSHB Hybridoma Product XAP-1 (Clone 3D2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Two cellular inductions involved in photoreceptor determination in the Xenopus retina.
Messersmith SL
Neuron 9.2 (1992 Aug): 357-72.