anti-Glorund 5B7

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$40.00
SKU: anti-Glorund 5B7
View product citations for antibody anti-Glorund 5B7 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Glorund
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Gavis, E.R.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Molecular Biology, Princeton University
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes:
Antigen Molecular Weight: Predicted: 61 kDa; Apparent 67 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: glo
Immunogen: Recombinant Glo
Alternate Gene Names: CG6946
Alternate Antibody Name: 5B7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9VGH5 
Immunogen Sequence: Full length protein
Entrez Gene ID: 41431 
Additional Characterization:  
Antibody Registry ID: AB_10570401 
Additional Information: 5B7 is specfic to Glo by western blot and immunostaining (no signal in glo mutant).
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Glorund 5B7 was deposited to the DSHB by Gavis, E.R. (DSHB Hybridoma Product anti-Glorund 5B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Glorund, a Drosophila hnRNP F/H homolog, is an ovarian repressor of nanos translation.
    Gavis ER
    Developmental cell 10.3 (2006 Mar): 291-301.

    Glorund interactions in the regulation of gurken and oskar mRNAs.
    Gavis ER
    Developmental biology 326.1 (2009 Feb 1): 68-74.

    IHC References
    IP References

    Glorund interactions in the regulation of gurken and oskar mRNAs.
    Gavis ER
    Developmental biology 326.1 (2009 Feb 1): 68-74.

    All References

    Glorund, a Drosophila hnRNP F/H homolog, is an ovarian repressor of nanos translation.
    Gavis ER
    Developmental cell 10.3 (2006 Mar): 291-301.

    Glorund interactions in the regulation of gurken and oskar mRNAs.
    Gavis ER
    Developmental biology 326.1 (2009 Feb 1): 68-74.

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