mAb 1E5.25.4

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SKU: mAb 1E5.25.4
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DSHB Data Sheet

Catalog Fields

Antigen: F-actin capping protein beta-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Cooper, J.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Med. School, St. Louis
Positive Tested Species Reactivity: Chicken, Human
Depositors Notes: For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.
Antigen Molecular Weight: 31.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CAPZB
Immunogen: Recombinant GST-C-terminus beta-1
Alternate Gene Names: CAPB, CAPPB, CAPZ
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: P14315 
Immunogen Sequence: a.a. 251-277
Entrez Gene ID: 832 
Additional Characterization:  
Antibody Registry ID: AB_531767 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 1E5.25.4 was deposited to the DSHB by Cooper, J.A. (DSHB Hybridoma Product mAb 1E5.25.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • All References
  • Initial Publication

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    IF References

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments.
    Cooper JA
    The Journal of cell biology 128.1-2 (1995 Jan): 61-70.

    Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
    Gregorio CC
    Molecular biology of the cell 19.5 (2008 May): 1837-47.

    Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis.
    Sanger JW
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 35-47.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    WB References

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments.
    Cooper JA
    The Journal of cell biology 128.1-2 (1995 Jan): 61-70.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
    Gregorio CC
    Molecular biology of the cell 19.5 (2008 May): 1837-47.

    Cyclic mechanical strain of myocytes modifies CapZβ1 post translationally via PKCε.
    Russell B
    Journal of muscle research and cell motility 36.4-5 (2015 Oct): 329-37.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    IP References

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    FB References

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments.
    Cooper JA
    The Journal of cell biology 128.1-2 (1995 Jan): 61-70.

    Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85.
    Brown MH
    The Journal of biological chemistry 278.25 (2003 Jun 20): 22396-403.

    All References

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments.
    Cooper JA
    The Journal of cell biology 128.1-2 (1995 Jan): 61-70.

    Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
    Gregorio CC
    Molecular biology of the cell 19.5 (2008 May): 1837-47.

    Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis.
    Sanger JW
    Cell motility and the cytoskeleton 62.1 (2005 Sep): 35-47.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Cyclic mechanical strain of myocytes modifies CapZβ1 post translationally via PKCε.
    Russell B
    Journal of muscle research and cell motility 36.4-5 (2015 Oct): 329-37.

    Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85.
    Brown MH
    The Journal of biological chemistry 278.25 (2003 Jun 20): 22396-403.

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