mAb 3F2.3

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$40.00
SKU: mAb 3F2.3
View product citations for antibody mAb 3F2.3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: F-actin capping protein beta-2 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Cooper, J.A.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Med. School, St. Louis
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes: For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.
Antigen Molecular Weight: 30.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CAPZB
Immunogen: Purified capping protein beta-2
Alternate Gene Names: CAPB, CAPPB, CAPZ
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: P14315 
Immunogen Sequence: Full length
Entrez Gene ID: 832 
Additional Characterization:
Antibody Registry ID:
Additional Information: 3F2 does not cross-react with the CapZ Beta-1 subunit.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 3F2.3 was deposited to the DSHB by Cooper, J.A. (DSHB Hybridoma Product mAb 3F2.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

    IF References

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular and cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    WB References

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Actin-capping protein promotes microtubule stability by antagonizing the actin activity of mDia1.
    Gundersen GG
    Molecular biology of the cell 23.20 (2012 Oct): 4032-40.

    Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
    Gregorio CC
    Molecular biology of the cell 19.5 (2008 May): 1837-47.

    Rapid and efficient purification of actin from nonmuscle sources.
    Cooper JA
    Cell motility and the cytoskeleton 39.2 (1998): 166-71.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular and cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    IHC References

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    IP References

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    FB References
    All References

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates.
    Cooper JA
    The Journal of cell biology 127.2 (1994 Oct): 453-65.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular and cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Actin-capping protein promotes microtubule stability by antagonizing the actin activity of mDia1.
    Gundersen GG
    Molecular biology of the cell 23.20 (2012 Oct): 4032-40.

    Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
    Gregorio CC
    Molecular biology of the cell 19.5 (2008 May): 1837-47.

    Rapid and efficient purification of actin from nonmuscle sources.
    Cooper JA
    Cell motility and the cytoskeleton 39.2 (1998): 166-71.

    Identification and characterization of an actin-binding site of CapZ.
    Cooper JA
    The Journal of cell biology 116.4 (1992 Feb): 923-31.

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