39

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SKU: 39

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 39
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619530 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: crude extracellular matrix prep
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Antigen Name: collagen type VI
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/11/1991
Antigen Molecular Weight: SDS PAGE: 255, 140 and 130 kDa bands
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins University
Antigen Species: chicken
Depositor Notes: IP note: works well when antibody bound to Sepharose beads. IHC note: present in all regions of muscle connective tissue; present bound to culture dishes after growth of primary chick muscle in tissue culture. Immunohistology: strong extracellular immunolabeling in many tissues.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:  
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 39)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    All References

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

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