39

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$40.00
SKU: 39
View product citations for antibody 39 on CiteAb

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Available: 42

DSHB Data Sheet

Catalog Fields

Antigen: collagen type VI
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University
Positive Tested Species Reactivity:  
Depositors Notes: IP note: works well when antibody bound to Sepharose beads. IHC note: present in all regions of muscle connective tissue; present bound to culture dishes after growth of primary chick muscle in tissue culture. Immunohistology: strong extracellular immunolabeling in many tissues.
Antigen Molecular Weight: SDS PAGE: 255, 140 and 130 kDa bands
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: crude extracellular matrix prep
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 39)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    All References

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

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