SP1.D8
Catalog Fields
Antigen: Collagen, Type I pro-peptide
Hybridoma Cells Available: Yes
Antigen Species: Sheep
Depositor: Furthmayr, H.
Isotype: MIgG1
Depositors Institution: Stanford University
Host Species: mouse
Depositors Notes: This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.
Positive Tested Species Reactivity: Avian, Bovine, Canine, Chicken, Chondrichthyes, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus, Zebrafish
Gene: COL1A1
Antigen Molecular Weight: 138.9 kDa
Alternate Gene Names: OI4
Immunogen: CNBr cleavage of pro-collagen type I from dermatosparactice sheep
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P02452
Epitope Location or Sequence: N-terminal residues in Type I collagen
Entrez Gene ID: 1277
Immunogen Sequence: Amino propeptide amino acids 1-9
Antibody Registry ID: AB_528438
Additional Information: The N-terminal propeptide extends from a.a 23-161.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SP1.D8 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product SP1.D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
18 References
Initial Publication
A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
Tuderman L
European journal of biochemistry 134.1 (1983 Jul 15): 183-9.