SP1.D8
Catalog Fields
Antigen: Collagen, Type I pro-peptide
Hybridoma Cells Available: Yes
Antigen Species: Sheep
Depositor: Furthmayr, H.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University
Positive Tested Species Reactivity: Avian, Bovine, Canine, Chicken, Chondrichthyes, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Turtle, Xenopus, Zebrafish
Depositors Notes: This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.
Predicted Species Reactivity:
Gene: COL1A1
Immunogen: CNBr cleavage of pro-collagen type I from dermatosparactice sheep
Alternate Gene Names: OI4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: N-terminal residues in Type I collagen
Uniprot ID: P02452
Immunogen Sequence: Amino propeptide amino acids 1-9
Entrez Gene ID: 1277
Antibody Registry ID: AB_528438
Additional Information: The N-terminal propeptide extends from a.a 23-161.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SP1.D8 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product SP1.D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
20 References
Initial Publication
A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
Tuderman L
European journal of biochemistry 134.1 (1983 Jul 15): 183-9.