SP1.D8

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$40.00
SKU: SP1.D8
View product citations for antibody SP1.D8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Collagen, Type I pro-peptide
Hybridoma Cells Available: Yes
Antigen Species: Sheep
Depositor: Furthmayr, H.
Isotype: MIgG1
Depositors Institution: Stanford University
Host Species: mouse
Depositors Notes: This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.
Positive Tested Species Reactivity: Avian, Bovine, Canine, Chicken, Chondrichthyes, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus, Zebrafish
Gene: COL1A1
Antigen Molecular Weight: 138.9 kDa
Alternate Gene Names: OI4
Immunogen: CNBr cleavage of pro-collagen type I from dermatosparactice sheep
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P02452 
Epitope Location or Sequence: N-terminal residues in Type I collagen
Entrez Gene ID: 1277 
Immunogen Sequence: Amino propeptide amino acids 1-9
Antibody Registry ID: AB_528438 
Additional Information: The N-terminal propeptide extends from a.a 23-161.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SP1.D8 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product SP1.D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
    Tuderman L
    European journal of biochemistry 134.1 (1983 Jul 15): 183-9.

    IF References

    An in vitro model for abnormal skeletal development in the lysosomal storage diseases.
    Alroy J
    Virchows Archiv : an international journal of pathology 426.2 (1995): 135-40.

    A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
    Tuderman L
    European journal of biochemistry 134.1 (1983 Jul 15): 183-9.

    Antiproliferative and antifibrotic effects of mimosine on adult cardiac fibroblasts.
    Dixon IM
    Biochimica et biophysica acta 1448.1 (1998 Nov 19): 51-60.

    Development of dermal denticles in skates (Chondrichthyes, Batoidea): patterning and cellular differentiation.
    Hall BK
    Journal of morphology 241.1 (1999 Jul): 61-81.

    Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts.
    Dixon IM
    Developmental dynamics : an official publication of the American Association of Anatomists 239.6 (2010 Jun): 1573-84.

    Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration.
    Mercader N
    Developmental biology 370.2 (2012 Oct 15): 173-86.

    Split Thickness Grafts Grow From Bottom Up in Large Skin Injuries.
    Ghahary A
    Journal of burn care & research : official publication of the American Burn Association 40.6 (2019 Oct 16): 727-733.

    WB References
    IHC References

    Microarterial synthetic graft repair: interstitial cellular components.
    Sandra A
    Microsurgery 11.4 (1990): 268-77.

    A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
    Tuderman L
    European journal of biochemistry 134.1 (1983 Jul 15): 183-9.

    Mechanical stimulation of tissue repair in the hydraulic bone chamber.
    Goldstein SA
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1295-302.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen.
    Griffith M
    Investigative ophthalmology & visual science 49.9 (2008 Sep): 3887-94.

    Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis.
    Scaglione S
    PloS one 11.2 (2016): e0148173.

    The increase of interfollicular epidermal stem cells and regulation of aryl hydrocarbon receptor and its repressors in the skin through hydroporation with anti-aging cocktail.
    Park KC
    Journal of cosmetic dermatology 18.4 (2019 Aug): 1133-1139.

    Endoplasmic reticulum stress is induced in growth plate hypertrophic chondrocytes in G610C mouse model of osteogenesis imperfecta.
    Otsuru S
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 235-240.

    Anterior Cruciate Ligament Tear Promotes Skeletal Muscle Myostatin Expression, Fibrogenic Cell Expansion, and a Decline in Muscle Quality.
    Fry CS
    The American journal of sports medicine 47.6 (2019 May): 1385-1395.

    IP References

    Intracellular trafficking and degradation of unassociated proalpha2 chains of collagen type I.
    Bienkowski RS
    Experimental cell research 296.2 (2004 Jun 10): 307-16.

    ELISA References

    Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts.
    Dixon IM
    Developmental dynamics : an official publication of the American Association of Anatomists 239.6 (2010 Jun): 1573-84.

    FFPE References

    Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts.
    Dixon IM
    Developmental dynamics : an official publication of the American Association of Anatomists 239.6 (2010 Jun): 1573-84.

    Epitope Map References

    A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
    Tuderman L
    European journal of biochemistry 134.1 (1983 Jul 15): 183-9.

    All References

    Microarterial synthetic graft repair: interstitial cellular components.
    Sandra A
    Microsurgery 11.4 (1990): 268-77.

    A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
    Tuderman L
    European journal of biochemistry 134.1 (1983 Jul 15): 183-9.

    Mechanical stimulation of tissue repair in the hydraulic bone chamber.
    Goldstein SA
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1295-302.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen.
    Griffith M
    Investigative ophthalmology & visual science 49.9 (2008 Sep): 3887-94.

    Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis.
    Scaglione S
    PloS one 11.2 (2016): e0148173.

    The increase of interfollicular epidermal stem cells and regulation of aryl hydrocarbon receptor and its repressors in the skin through hydroporation with anti-aging cocktail.
    Park KC
    Journal of cosmetic dermatology 18.4 (2019 Aug): 1133-1139.

    Endoplasmic reticulum stress is induced in growth plate hypertrophic chondrocytes in G610C mouse model of osteogenesis imperfecta.
    Otsuru S
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 235-240.

    Anterior Cruciate Ligament Tear Promotes Skeletal Muscle Myostatin Expression, Fibrogenic Cell Expansion, and a Decline in Muscle Quality.
    Fry CS
    The American journal of sports medicine 47.6 (2019 May): 1385-1395.

    An in vitro model for abnormal skeletal development in the lysosomal storage diseases.
    Alroy J
    Virchows Archiv : an international journal of pathology 426.2 (1995): 135-40.

    Antiproliferative and antifibrotic effects of mimosine on adult cardiac fibroblasts.
    Dixon IM
    Biochimica et biophysica acta 1448.1 (1998 Nov 19): 51-60.

    Development of dermal denticles in skates (Chondrichthyes, Batoidea): patterning and cellular differentiation.
    Hall BK
    Journal of morphology 241.1 (1999 Jul): 61-81.

    Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts.
    Dixon IM
    Developmental dynamics : an official publication of the American Association of Anatomists 239.6 (2010 Jun): 1573-84.

    Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration.
    Mercader N
    Developmental biology 370.2 (2012 Oct 15): 173-86.

    Split Thickness Grafts Grow From Bottom Up in Large Skin Injuries.
    Ghahary A
    Journal of burn care & research : official publication of the American Burn Association 40.6 (2019 Oct 16): 727-733.

    UV-induced inhibition of adipokine production in subcutaneous fat aggravates dermal matrix degradation in human skin.
    Chung JH
    Scientific reports 6. (2016 May 10): 25616.

    Ultraviolet irradiation induces thrombospondin-1 which attenuates type I procollagen downregulation in human dermal fibroblasts.
    Chung JH
    Journal of dermatological science 59.1 (2010 Jul): 16-24.

    Intracellular trafficking and degradation of unassociated proalpha2 chains of collagen type I.
    Bienkowski RS
    Experimental cell research 296.2 (2004 Jun 10): 307-16.

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