SP1.D8
Catalog Fields
Clone ID/Product Name: SP1.D8
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: COL1A1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 1277
Clonality: Monoclonal
Immunogen: CNBr cleavage of pro-collagen type I from dermatosparactice sheep
Clone:
Immunogen Sequence: Amino propeptide amino acids 1-9
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Collagen, Type I pro-peptide
Epitope Location or Sequence: N-terminal residues in Type I collagen
Alternate Antigen Name:
Deposit Date: 8/25/1988
Antigen Molecular Weight: 138.9 kDa
Depositor: Furthmayr, H.
Antigen Sequence:
Depositor Institution: Yale University
Antigen Species: Sheep
Depositor Notes: This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Avian, Axolotl, Bovine, Canine, Chicken, Chondrichthyes, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Turtle, Xenopus, Zebrafish
Additional Information: The N-terminal propeptide extends from a.a 23-161.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SP1.D8 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product SP1.D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
Tuderman L
European journal of biochemistry 134.1 (1983 Jul 15): 183-9.