3B2
Catalog Fields
Antigen: collagen type III
Hybridoma Cells Available:
Antigen Species: chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Univ. of Alabama at Birmingham
Positive Tested Species Reactivity: Chicken
Depositors Notes: 3B2 appears to be chicken-specific and doesn't cross react with quail tissue or a variety of mammalian species.
Antigen Molecular Weight: Predicted: 78 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: COL3A1
Immunogen: Type III collagen from chicken gizzard.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: The epitope is located approximately one-fifth of the distance from the amino terminus of the collagen molecule (see reference in Additional Information).
Uniprot ID: P12105
Immunogen Sequence: Full length protein
Entrez Gene ID: 396340
Additional Characterization:
Antibody Registry ID: AB_528166
Additional Information: This antibody was originally described in Swasdison, S., Mayne, P.M., Wright, D.W., Accavitti, M.A., Fitch, J.M., Linsenmayer, T.F. and Mayne, R. (1992); Monoclonal antibodies that distinguish avian type I and type III collagens: Isolation, characterization and immunolocalization in various issues; Matrix 12, 56-65. ELISA and western blotting were performed in this article.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 3B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Cell and tissue research
Mayne R
Cell and tissue research 257.3 (1989 Sep): 537-43.