3B2
Catalog Fields
Clone ID/Product Name: 3B2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: COL3A1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 396340
Clonality: Monoclonal
Immunogen: Type III collagen from chicken gizzard.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: collagen type III
Epitope Location or Sequence: The epitope is located approximately one-fifth of the distance from the amino terminus of the collagen molecule (see reference in Additional Information).
Alternate Antigen Name:
Deposit Date: 5/20/1993
Antigen Molecular Weight: Predicted: 78 kDa
Depositor: Mayne, R.
Antigen Sequence:
Depositor Institution: University of Alabama at Birmingham
Antigen Species: chicken
Depositor Notes: 3B2 appears to be chicken-specific and doesn't cross react with quail tissue or a variety of mammalian species.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chicken
Additional Information: This antibody was originally described in Swasdison, S., Mayne, P.M., Wright, D.W., Accavitti, M.A., Fitch, J.M., Linsenmayer, T.F. and Mayne, R. (1992); Monoclonal antibodies that distinguish avian type I and type III collagens: Isolation, characterization and immunolocalization in various issues; Matrix 12, 56-65. ELISA and western blotting were performed in this article.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 3B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Location of the integrin complex and extracellular matrix molecules at the chicken myotendinous junction.
Mayne R
Cell and tissue research 257.3 (1989 Sep): 537-43.