3B2

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$40.00
SKU: 3B2-s
View product citations for antibody 3B2 on CiteAb

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Available: 133

DSHB Data Sheet

Catalog Fields

Antigen: collagen type III
Hybridoma Cells Available:
Antigen Species: chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Univ. of Alabama at Birmingham
Positive Tested Species Reactivity: Chicken
Depositors Notes: 3B2 appears to be chicken-specific and doesn't cross react with quail tissue or a variety of mammalian species.
Antigen Molecular Weight: Predicted: 78 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: COL3A1
Immunogen: Type III collagen from chicken gizzard.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: The epitope is located approximately one-fifth of the distance from the amino terminus of the collagen molecule (see reference in Additional Information).
Uniprot ID: P12105 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396340 
Additional Characterization:
Antibody Registry ID: AB_528166 
Additional Information: This antibody was originally described in Swasdison, S., Mayne, P.M., Wright, D.W., Accavitti, M.A., Fitch, J.M., Linsenmayer, T.F. and Mayne, R. (1992); Monoclonal antibodies that distinguish avian type I and type III collagens: Isolation, characterization and immunolocalization in various issues; Matrix 12, 56-65. ELISA and western blotting were performed in this article.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 3B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Type II collagen is transiently expressed during avian cardiac valve morphogenesis.
    Solursh M
    Developmental dynamics : an official publication of the American Association of Anatomists 200.4 (1994 Aug): 294-304.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    IHC References

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix.
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    A novel aspect of the structure of the avian thymic medulla.
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Impact of divergent selection for ultimate pH of pectoralis major muscle on biochemical, histological, and sensorial attributes of broiler meat.
    Berri C
    Journal of animal science 93.9 (2015 Sep): 4524-31.

    Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken.
    Oláh I
    Journal of anatomy 211.3 (2007 Sep): 407-11.

    Extracellular matrix remodeling and organization in developing and diseased aortic valves.
    Yutzey KE
    Circulation research 98.11 (2006 Jun 9): 1431-8.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Role of canonical Wnt-signalling in joint formation.
    Hartmann C
    European cells and materials 12. (2006 Nov 17): 71-80.

    All References

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix.
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    A novel aspect of the structure of the avian thymic medulla.
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Impact of divergent selection for ultimate pH of pectoralis major muscle on biochemical, histological, and sensorial attributes of broiler meat.
    Berri C
    Journal of animal science 93.9 (2015 Sep): 4524-31.

    Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken.
    Oláh I
    Journal of anatomy 211.3 (2007 Sep): 407-11.

    Extracellular matrix remodeling and organization in developing and diseased aortic valves.
    Yutzey KE
    Circulation research 98.11 (2006 Jun 9): 1431-8.

    Role of canonical Wnt-signalling in joint formation.
    Hartmann C
    European cells and materials 12. (2006 Nov 17): 71-80.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Type II collagen is transiently expressed during avian cardiac valve morphogenesis.
    Solursh M
    Developmental dynamics : an official publication of the American Association of Anatomists 200.4 (1994 Aug): 294-304.

    Location of the integrin complex and extracellular matrix molecules at the chicken myotendinous junction.
    Mayne R
    Cell and tissue research 257.3 (1989 Sep): 537-43.

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