3B2
Catalog Fields
Antigen: collagen type III
Hybridoma Cells Available: No
Antigen Species: chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Alabama at Birmingham
Positive Tested Species Reactivity: Chicken
Depositors Notes: 3B2 appears to be chicken-specific and doesn't cross react with quail tissue or a variety of mammalian species.
Predicted Species Reactivity:
Gene: COL3A1
Immunogen: Type III collagen from chicken gizzard.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: The epitope is located approximately one-fifth of the distance from the amino terminus of the collagen molecule (see reference in Additional Information).
Uniprot ID: P12105
Immunogen Sequence: Full length protein
Entrez Gene ID: 396340
Antibody Registry ID: AB_528166
Additional Information: This antibody was originally described in Swasdison, S., Mayne, P.M., Wright, D.W., Accavitti, M.A., Fitch, J.M., Linsenmayer, T.F. and Mayne, R. (1992); Monoclonal antibodies that distinguish avian type I and type III collagens: Isolation, characterization and immunolocalization in various issues; Matrix 12, 56-65. ELISA and western blotting were performed in this article.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 3B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Location of the integrin complex and extracellular matrix molecules at the chicken myotendinous junction.
Mayne R
Cell and tissue research 257.3 (1989 Sep): 537-43.