(0) No Reviews yet
SKU: 9/30/8-A-4-s
View product citations for antibody 9/30/8-A-4 on CiteAb

In Stock

Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: link protein
Hybridoma Cells Available: No
Antigen Species: rat
Depositor: Caterson, B.
Isotype: MIgG2b
Antigen Sequence: about 40 kDa
Host Species: mouse
Depositors Institution: Cardiff University
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Human, Porcine, Rabbit, Rat
Depositors Notes: Recognizes 2 epitopes in the polypeptide backbone of rat chondrosarcoma link protein. IHC note: in proteoglycan aggregates, epitopes are sequestered; the proteoglycan must be reduced and alkylated. Recognizes link protein isolated from rat, bovine, human, chicken, rabbit, porcine, and canine hyaline cartilages.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: purified rat chondrosarcoma link protein 2
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2248142 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9/30/8-A-4 was deposited to the DSHB by Caterson, B. (DSHB Hybridoma Product 9/30/8-A-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review