12/21/1-C-6

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$40.00
SKU: 12/21/1-C-6-s
View product citations for antibody 12/21/1-C-6 on CiteAb

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Available: 88

DSHB Data Sheet

Catalog Fields

Antigen: Aggrecan core protein
Hybridoma Cells Available:
Antigen Species: Rat
Depositor: Caterson, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Nat. Institute of Dental Research, NIH/ Cardiff University
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Depositors Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Antigen Molecular Weight: 221.9kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ACAN
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Alternate Gene Names: AGC1, CSPG1, CSPGCP, MSK16
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: aggrecan
Epitope Mapped:
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q61282 
Immunogen Sequence: Protein domain
Entrez Gene ID: 176 
Additional Characterization:
Antibody Registry ID: AB_531912 
Additional Information: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12/21/1-C-6 was deposited to the DSHB by Caterson, B. (DSHB Hybridoma Product 12/21/1-C-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • IHC References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Characterization of a conserved apoptotic marker expressed in Caenorhabditis elegans phagocytic cells.
    Hermann GJ
    Biochemical and biophysical research communications 335.4 (2005 Oct 7): 1231-8.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    IHC References

    Smad4 regulates growth plate matrix production and chondrocyte polarity.
    Alliston T
    Biology open 6.3 (2017 Mar 15): 358-364.

    Effects of cell type and configuration on anabolic and catabolic activity in 3D co-culture of mesenchymal stem cells and nucleus pulposus cells.
    Lotz JC
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 35.1 (2017 Jan): 61-73.

    Heterodimeric BMP-2/7 for nucleus pulposus regeneration-In vitro and ex vivo studies.
    Grad S
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 35.1 (2017 Jan): 51-60.

    Sulfated hydrogel matrices direct mitogenicity and maintenance of chondrocyte phenotype through activation of FGF signaling.
    Zenobi-Wong M
    Advanced functional materials 26.21 (2016 Jun 7): 3649-3662.

    Establishment of a novel dwarf rat strain: cartilage calcification insufficient (CCI) rats.
    Tanuma J
    Experimental animals 64.2 (2015): 121-8.

    The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair.
    Hall AC
    Osteoarthritis and cartilage 23.3 (2015 Mar): 469-77.

    Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers.
    Zenobi-Wong M
    Tissue engineering. Part A 20.9-10 (2014 May): 1454-64.

    An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses.
    Cho BH
    European journal of histochemistry : EJH 57.4 (2013 Dec 2): e39.

    Sonic hedgehog signaling directly targets Hyaluronic Acid Synthase 2, an essential regulator of phalangeal joint patterning.
    Chiang C
    Developmental biology 375.2 (2013 Mar 15): 160-71.

    Chondrocyte-intrinsic Smad3 represses Runx2-inducible matrix metalloproteinase 13 expression to maintain articular cartilage and prevent osteoarthritis.
    Alliston T
    Arthritis and rheumatism 64.10 (2012 Oct): 3278-89.

    Differential response of human bone marrow stromal cells to either TGF-β(1) or rhGDF-5.
    Grad S
    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 20.6 (2011 Jun): 962-71.

    Functional lateral shift of the mandible effects on the expression of ECM in rat temporomandibular cartilage.
    Soma K
    The Angle orthodontist 79.4 (2009 Jul): 652-9.

    JAWS coordinates chondrogenesis and synovial joint positioning.
    Harland RM
    Development (Cambridge, England) 135.13 (2008 Jul): 2215-20.

    An immunohistochemical study of the extracellular matrix of entheses associated with the human pisiform bone.
    Benjamin M
    Journal of anatomy 212.5 (2008 May): 645-53.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.
    Benjamin M
    Investigative ophthalmology and visual science 43.5 (2002 May): 1330-4.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints.
    Benjamin M
    The Anatomical record 256.2 (1999 Oct 1): 139-45.

    Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma.
    Caterson B
    Archives of biochemistry and biophysics 298.2 (1992 Nov 1): 349-60.

    Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions.
    Varoga DJ
    BMC musculoskeletal disorders 16. (2015 Sep 12): 250.

    FFPE References
    FACS References
    All References

    Smad4 regulates growth plate matrix production and chondrocyte polarity.
    Alliston T
    Biology open 6.3 (2017 Mar 15): 358-364.

    Effects of cell type and configuration on anabolic and catabolic activity in 3D co-culture of mesenchymal stem cells and nucleus pulposus cells.
    Lotz JC
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 35.1 (2017 Jan): 61-73.

    Heterodimeric BMP-2/7 for nucleus pulposus regeneration-In vitro and ex vivo studies.
    Grad S
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 35.1 (2017 Jan): 51-60.

    Sulfated hydrogel matrices direct mitogenicity and maintenance of chondrocyte phenotype through activation of FGF signaling.
    Zenobi-Wong M
    Advanced functional materials 26.21 (2016 Jun 7): 3649-3662.

    Establishment of a novel dwarf rat strain: cartilage calcification insufficient (CCI) rats.
    Tanuma J
    Experimental animals 64.2 (2015): 121-8.

    The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair.
    Hall AC
    Osteoarthritis and cartilage 23.3 (2015 Mar): 469-77.

    Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers.
    Zenobi-Wong M
    Tissue engineering. Part A 20.9-10 (2014 May): 1454-64.

    An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses.
    Cho BH
    European journal of histochemistry : EJH 57.4 (2013 Dec 2): e39.

    Sonic hedgehog signaling directly targets Hyaluronic Acid Synthase 2, an essential regulator of phalangeal joint patterning.
    Chiang C
    Developmental biology 375.2 (2013 Mar 15): 160-71.

    Chondrocyte-intrinsic Smad3 represses Runx2-inducible matrix metalloproteinase 13 expression to maintain articular cartilage and prevent osteoarthritis.
    Alliston T
    Arthritis and rheumatism 64.10 (2012 Oct): 3278-89.

    Differential response of human bone marrow stromal cells to either TGF-β(1) or rhGDF-5.
    Grad S
    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 20.6 (2011 Jun): 962-71.

    Functional lateral shift of the mandible effects on the expression of ECM in rat temporomandibular cartilage.
    Soma K
    The Angle orthodontist 79.4 (2009 Jul): 652-9.

    JAWS coordinates chondrogenesis and synovial joint positioning.
    Harland RM
    Development (Cambridge, England) 135.13 (2008 Jul): 2215-20.

    An immunohistochemical study of the extracellular matrix of entheses associated with the human pisiform bone.
    Benjamin M
    Journal of anatomy 212.5 (2008 May): 645-53.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.
    Benjamin M
    Investigative ophthalmology and visual science 43.5 (2002 May): 1330-4.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints.
    Benjamin M
    The Anatomical record 256.2 (1999 Oct 1): 139-45.

    Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma.
    Caterson B
    Archives of biochemistry and biophysics 298.2 (1992 Nov 1): 349-60.

    Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions.
    Varoga DJ
    BMC musculoskeletal disorders 16. (2015 Sep 12): 250.

    Characterization of a conserved apoptotic marker expressed in Caenorhabditis elegans phagocytic cells.
    Hermann GJ
    Biochemical and biophysical research communications 335.4 (2005 Oct 7): 1231-8.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

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