12/21/1-C-6
Catalog Fields
Antigen: Aggrecan core protein
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Caterson, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Nat. Institute of Dental Research, NIH/ Cardiff University
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Depositors Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Predicted Species Reactivity:
Gene: ACAN
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Alternate Gene Names: AGC1, CSPG1, CSPGCP, MSK16
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: aggrecan
Epitope Mapped: No
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q61282
Immunogen Sequence: Protein domain
Entrez Gene ID: 176
Antibody Registry ID: AB_531912
Additional Information: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12/21/1-C-6 was deposited to the DSHB by Caterson, B. (DSHB Hybridoma Product 12/21/1-C-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
25 References
Initial Publication
Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma.
Caterson B
Archives of biochemistry and biophysics 298.2 (1992 Nov 1): 349-60.