12/21/1-C-6
Catalog Fields
Antigen: Aggrecan core protein
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Caterson, B.
Isotype: MIgG1
Depositors Institution: Nat. Institute of Dental Research, NIH/ Cardiff University
Host Species: mouse
Depositors Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Gene: ACAN
Antigen Molecular Weight: 221.9kDa
Alternate Gene Names: AGC1, CSPG1, CSPGCP, MSK16
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Clonality: Monoclonal
Alternate Antigen Name: aggrecan
Epitope Mapped: No
Myeloma Strain: X63-Ag8.653
Immunogen Sequence: Protein domain
Uniprot ID: Q61282
Additional Information: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Entrez Gene ID: 176
Antibody Registry ID: AB_531912
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12/21/1-C-6 was deposited to the DSHB by Caterson, B. (DSHB Hybridoma Product 12/21/1-C-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
25 References
Initial Publication
Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma.
Caterson B
Archives of biochemistry and biophysics 298.2 (1992 Nov 1): 349-60.