Antibody Name: 12/21/1-C-6
Antigen:Aggrecan core protein
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: X63-Ag8.653
Antibody Registry / RRID: AB_531912
Available to For-Profits: Yes
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Immunogen Sequence: Protein domain
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Predicted Reactivity: —
Depositor
Depositor Name: B. Caterson
Institution: Cardiff University, Nat. Institute of Dental Research, NIH
Deposit Date: June 1, 1986
Depositor Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Recommended Applications
FFPE
IF
IHC
Each lab must optimize concentration per application and species. Full usage recommendations →
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