12/21/1-C-6
Catalog Fields
Clone ID/Product Name: 12/21/1-C-6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ACAN
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 176
Clonality: Monoclonal
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Clone:
Immunogen Sequence: Protein domain
Myeloma Strain: X63-Ag8.653
Epitope Mapped: No
Antigen Name: Aggrecan core protein
Epitope Location or Sequence:
Alternate Antigen Name: aggrecan
Deposit Date: 6/1/1986
Antigen Molecular Weight: 221.9kDa
Depositor: Caterson, B.
Antigen Sequence:
Depositor Institution: Cardiff University, Nat. Institute of Dental Research, NIH
Antigen Species: Rat
Depositor Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Additional Information: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry