CIIC1

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$40.00
SKU: CIIC1
View product citations for antibody CIIC1 on CiteAb

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Available: 173

DSHB Data Sheet

Catalog Fields

Antigen: collagen type II
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Holmdahl, R. / Rubin, K.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Uppsala
Positive Tested Species Reactivity: Bovine, Chick, Fish, Human, Mouse, Ovine, Rabbit, Rat, Xenopus
Depositors Notes: Stains hyaluronidase-treated fetal and adult mouse and human cartilage in vitro as well as mouse cartilage in vivo. This antibody recognizes native triple-helical type II collagen. It does not bind to denatured type II collagen (PMID: 25005029).
Antigen Molecular Weight: Predicted: 125 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: COL2A1
Immunogen: native chick type I1 collagen
Alternate Gene Names:
Alternate Antibody Name: CIICI, C1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: The ARGLT epitope is located at aa 359-363 of the human collagen II triple helical region
Uniprot ID: H9L2B7 
Immunogen Sequence: Full length protein
Entrez Gene ID: 395069 
Additional Characterization:
Antibody Registry ID: AB_528164 
Additional Information: The CIIC1 antibody induces sub clinical arthritis if injected at low (0.5 mg) doses and clinical arthritis in mice if injected at higher doses and in combination with other antibodies (see [PMID: 16125192]).
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CIIC1 was deposited to the DSHB by Holmdahl, R. / Rubin, K. (DSHB Hybridoma Product CIIC1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

28 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Fibrocartilages in the extensor tendons of the interphalangeal joints of human toes.
    Benjamin M
    The Anatomical record 252.2 (1998 Oct): 264-70.

    Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints.
    Benjamin M
    The Anatomical record 256.2 (1999 Oct 1): 139-45.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Three-dimensional reconstructions of the Achilles tendon insertion in man.
    Benjamin M
    Journal of anatomy 200.Pt 2 (2002 Feb): 145-52.

    Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.
    Benjamin M
    Investigative ophthalmology and visual science 43.5 (2002 May): 1330-4.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Efficient promotion of collagen antibody induced arthritis (CAIA) using four monoclonal antibodies specific for the major epitopes recognized in both collagen induced arthritis and rheumatoid arthritis.
    Holmdahl R
    Journal of immunological methods 304.1-2 (2005 Sep): 126-36.

    Influence of various alginate brands on the redifferentiation of dedifferentiated bovine articular chondrocytes in alginate bead culture under high and low oxygen tension.
    Kurz B
    Tissue engineering 10.11-12 (2004 Nov-Dec): 1796-805.

    Pectoral fin and girdle development in the basal actinopterygians Polyodon spathula and Acipenser transmontanus.
    Force A
    Journal of morphology 262.2 (2004 Nov): 608-28.

    Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers.
    Fiorini M
    Biomaterials science 4.11 (2016 Nov 18): 1691-1703.

    Viability and Tissue Quality of Cartilage Flaps From Patients With Femoroacetabular Hip Impingement: A Matched-Control Comparison.
    Pascual-Garrido C
    Orthopaedic journal of sports medicine 5.8 (2017 Aug): 2325967117723608.

    ELISA References
    FFPE References
    Epitope Map References
    All References

    Fibrocartilages in the extensor tendons of the interphalangeal joints of human toes.
    Benjamin M
    The Anatomical record 252.2 (1998 Oct): 264-70.

    Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints.
    Benjamin M
    The Anatomical record 256.2 (1999 Oct 1): 139-45.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Three-dimensional reconstructions of the Achilles tendon insertion in man.
    Benjamin M
    Journal of anatomy 200.Pt 2 (2002 Feb): 145-52.

    Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.
    Benjamin M
    Investigative ophthalmology and visual science 43.5 (2002 May): 1330-4.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Efficient promotion of collagen antibody induced arthritis (CAIA) using four monoclonal antibodies specific for the major epitopes recognized in both collagen induced arthritis and rheumatoid arthritis.
    Holmdahl R
    Journal of immunological methods 304.1-2 (2005 Sep): 126-36.

    Influence of various alginate brands on the redifferentiation of dedifferentiated bovine articular chondrocytes in alginate bead culture under high and low oxygen tension.
    Kurz B
    Tissue engineering 10.11-12 (2004 Nov-Dec): 1796-805.

    Pectoral fin and girdle development in the basal actinopterygians Polyodon spathula and Acipenser transmontanus.
    Force A
    Journal of morphology 262.2 (2004 Nov): 608-28.

    Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers.
    Fiorini M
    Biomaterials science 4.11 (2016 Nov 18): 1691-1703.

    Viability and Tissue Quality of Cartilage Flaps From Patients With Femoroacetabular Hip Impingement: A Matched-Control Comparison.
    Pascual-Garrido C
    Orthopaedic journal of sports medicine 5.8 (2017 Aug): 2325967117723608.

    Maturation of myogenic and chondrogenic cells in the presomitic mesoderm of the chick embryo.
    Lash JW
    Experimental cell research 211.2 (1994 Apr): 263-74.

    The teratogenic effects of valproic acid in human chondrogenesis in vitro.
    Hitt DC
    Teratology 49.3 (1994 Mar): 208-17.

    An in vitro model for abnormal skeletal development in the lysosomal storage diseases.
    Alroy J
    Virchows Archiv : an international journal of pathology 426.2 (1995): 135-40.

    Coculture between periosteal explants and articular chondrocytes induces expression of TGF-beta1 and collagen I.
    Anders S
    Rheumatology (Oxford, England) 49.2 (2010 Feb): 218-30.

    Enhanced tissue integration during cartilage repair in vitro can be achieved by inhibiting chondrocyte death at the wound edge.
    Archer CW
    Tissue engineering. Part A 15.7 (2009 Jul): 1739-49.

    Extracellular matrix of connective tissues in the heads of teleosts.
    Ralphs JR
    Journal of anatomy 179. (1991 Dec): 137-48.

    Transglutaminase-catalyzed matrix cross-linking in differentiating cartilage: identification of osteonectin as a major glutaminyl substrate.
    Paulsson M
    The Journal of cell biology 129.3 (1995 May): 881-92.

    Effect of passage number and collagen type on the proliferative, biosynthetic, and contractile activity of adult canine articular chondrocytes in type I and II collagen-glycosaminoglycan matrices in vitro.
    Spector M
    Tissue engineering 10.1-2 (2004 Jan-Feb): 119-27.

    Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis.
    Holmdahl R
    European journal of immunology 35.5 (2005 May): 1643-52.

    A murine Ig light chain transgene reveals IGKV3 gene contributions to anti-collagen types IV and II specificities.
    Foster MH
    Molecular immunology 91. (2017 Nov): 49-56.

    Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies.
    Wigzell H
    Arthritis and rheumatism 29.3 (1986 Mar): 400-10.

    Development of functional specializations within the maturing rabbit flexor digitorum profundus tendon.
    Daniel JC
    Connective tissue research 30.1 (1993): 37-57.

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