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View product citations for antibody ADL46 on CiteAb

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DSHB Data Sheet

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Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Host Species: mouse
Depositors Notes: Does not react with Sf9 lamin. Reacts with all Dm isoforms. Epitope between aa 336-410 (coil 2).
Positive Tested Species Reactivity: Drosophila
Gene: Lam
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Immunogen: Recombinant total protein
Clonality: Monoclonal
Alternate Antibody Name: ADL84; alphaL22-28
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 336-410 [Depositor Notes]
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Antibody Registry ID: AB_528335 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL46 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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