ADL46

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$40.00
SKU: ADL46
View product citations for antibody ADL46 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not react with Sf9 lamin. Reacts with all Dm isoforms. Epitope between aa 336-410 (coil 2).
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL84; alphaL22-28
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 336-410 [Depositor Notes]
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:  
Antibody Registry ID: AB_528335 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL46 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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