Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not react with Sf9 lamin. Reacts only with Dm1 and Dmmit, not Dm2 or Dmmit-s; phosphorylation of S(25) abolishes reactivity; does not react with lamin C.
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL84; alphaL22-28
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 22-28; RPPSAGP
Uniprot ID: P08928
Immunogen Sequence: Total protein
Entrez Gene ID: 33782
Antibody Registry ID: AB_528338
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL84.12 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL84.12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References