ADL84.12

(0) No Reviews yet
$40.00
SKU: ADL84.12
View product citations for antibody ADL84.12 on CiteAb

In Stock

Available: 233

DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not react with Sf9 lamin. Reacts only with Dm1 and Dmmit, not Dm2 or Dmmit-s; phosphorylation of S(25) abolishes reactivity; does not react with lamin C.
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL84; alphaL22-28
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 22-28; RPPSAGP
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:  
Antibody Registry ID: AB_528338 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL84.12 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL84.12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells.
    Sugiyama S
    Experimental cell research 315.7 (2009 Apr 15): 1181-9.

    Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene.
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    A comparative study of Drosophila and human A-type lamins.
    Wallrath LL
    PloS one 4.10 (2009 Oct 26): e7564.

    Disassembly of the Drosophila nuclear lamina in a homologous cell-free system.
    Fisher PA
    Journal of cell science 108 ( Pt 5). (1995 May): 2027-35.

    A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila.
    Krasnow MA
    Nature cell biology 3.9 (2001 Sep): 848-51.

    A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers.
    Stuurman N
    Journal of structural biology 123.1 (1998 Sep): 56-66.

    A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells.
    Sugiyama S
    Experimental cell research 315.7 (2009 Apr 15): 1181-9.

    Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
    Sullivan W
    PLoS pathogens 5.3 (2009 Mar): e1000343.

    Role of histone deacetylases in gene regulation at nuclear lamina.
    Nurminsky DI
    PloS one 7.11 (2012): e49692.

    Caspases function in autophagic programmed cell death in Drosophila.
    Baehrecke EH
    Development (Cambridge, England) 131.2 (2004 Jan): 275-84.

    The axon guidance gene lola is required for programmed cell death in the Drosophila ovary.
    McCall K
    Developmental biology 304.2 (2007 Apr 15): 771-85.

    Med24 and Mdh2 are required for Drosophila larval salivary gland cell death.
    Thummel CS
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 954-64.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    WB References

    Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    In vivo phosphorylation of Drosophila melanogaster nuclear lamins during both interphase and mitosis.
    Fisher PA
    Cellular & molecular biology letters 7.3 (2002): 859-76.

    Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.
    Geyer PK
    Genetics 197.2 (2014 Jun): 653-65.

    Caspases function in autophagic programmed cell death in Drosophila.
    Baehrecke EH
    Development (Cambridge, England) 131.2 (2004 Jan): 275-84.

    IP References
    Epitope Map References
    All References

    Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells.
    Sugiyama S
    Experimental cell research 315.7 (2009 Apr 15): 1181-9.

    Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene.
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    A comparative study of Drosophila and human A-type lamins.
    Wallrath LL
    PloS one 4.10 (2009 Oct 26): e7564.

    Disassembly of the Drosophila nuclear lamina in a homologous cell-free system.
    Fisher PA
    Journal of cell science 108 ( Pt 5). (1995 May): 2027-35.

    A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila.
    Krasnow MA
    Nature cell biology 3.9 (2001 Sep): 848-51.

    A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers.
    Stuurman N
    Journal of structural biology 123.1 (1998 Sep): 56-66.

    Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
    Sullivan W
    PLoS pathogens 5.3 (2009 Mar): e1000343.

    Role of histone deacetylases in gene regulation at nuclear lamina.
    Nurminsky DI
    PloS one 7.11 (2012): e49692.

    Caspases function in autophagic programmed cell death in Drosophila.
    Baehrecke EH
    Development (Cambridge, England) 131.2 (2004 Jan): 275-84.

    The axon guidance gene lola is required for programmed cell death in the Drosophila ovary.
    McCall K
    Developmental biology 304.2 (2007 Apr 15): 771-85.

    Med24 and Mdh2 are required for Drosophila larval salivary gland cell death.
    Thummel CS
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 954-64.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    In vivo phosphorylation of Drosophila melanogaster nuclear lamins during both interphase and mitosis.
    Fisher PA
    Cellular & molecular biology letters 7.3 (2002): 859-76.

    Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.
    Geyer PK
    Genetics 197.2 (2014 Jun): 653-65.

    Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding.
    Fisher PA
    Journal of structural biology 117.1 (1996 Jul-Aug): 1-15.

    Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation.
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review