ADL67.10

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$40.00
SKU: ADL67.10
View product citations for antibody ADL67.10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila, Mosquito, Spodoptera frugiperda
Depositors Notes: Reacts with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL67; alphaL528-622
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 548-620 [Depositor Notes] or aa 528-622 [PMID 8776884].
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:
Antibody Registry ID: AB_528336 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL67.10 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL67.10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Journal of cell science
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    IF References

    Journal of cell science
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    The Journal of cell biology
    Schmitt B
    The Journal of cell biology 137.5 (1997 Jun 2): 1001-16.

    Developmental biology
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Nature genetics
    van Steensel B
    Nature genetics 38.9 (2006 Sep): 1005-14.

    Viruses
    Ji S
    Viruses 8.5 (2016 May 7): .

    PloS one
    Rzepecki R
    PloS one 7.2 (2012): e32649.

    Genetics
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    PloS one
    Prantera G
    PloS one 11.3 (2016): e0151231.

    Journal of molecular biology
    Shivashankar GV
    Journal of molecular biology 427.3 (2015 Feb 13): 695-706.

    BMC genomics
    Sharakhov IV
    BMC genomics 11. (2010 Aug 4): 459.

    Genetics
    Goldberg ML
    Genetics 191.4 (2012 Aug): 1181-97.

    WB References

    Journal of cell science
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Journal of cell science
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    Journal of cell science
    Kadrmas JL
    Journal of cell science 125.Pt 13 (2012 Jul 1): 3185-94.

    The Journal of cell biology
    Schmitt B
    The Journal of cell biology 137.5 (1997 Jun 2): 1001-16.

    Developmental biology
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Nature genetics
    van Steensel B
    Nature genetics 38.9 (2006 Sep): 1005-14.

    Viruses
    Ji S
    Viruses 8.5 (2016 May 7): .

    DNA repair
    Fisher PA
    DNA repair 4.9 (2005 Aug 15): 971-82.

    IHC References

    Journal of cell science
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Current biology : CB
    Fanto M
    Current biology : CB 27.23 (2017 Dec 4): 3626-3642.e6.

    IP References

    Journal of structural biology
    Fisher PA
    Journal of structural biology 117.1 (1996 Jul-Aug): 1-15.

    Journal of cell science
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    Epitope Map References

    Journal of structural biology
    Fisher PA
    Journal of structural biology 117.1 (1996 Jul-Aug): 1-15.

    All References

    Journal of cell science
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Current biology : CB
    Fanto M
    Current biology : CB 27.23 (2017 Dec 4): 3626-3642.e6.

    The Journal of cell biology
    Schmitt B
    The Journal of cell biology 137.5 (1997 Jun 2): 1001-16.

    Developmental biology
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Nature genetics
    van Steensel B
    Nature genetics 38.9 (2006 Sep): 1005-14.

    Viruses
    Ji S
    Viruses 8.5 (2016 May 7): .

    PloS one
    Rzepecki R
    PloS one 7.2 (2012): e32649.

    Genetics
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    PloS one
    Prantera G
    PloS one 11.3 (2016): e0151231.

    Journal of molecular biology
    Shivashankar GV
    Journal of molecular biology 427.3 (2015 Feb 13): 695-706.

    BMC genomics
    Sharakhov IV
    BMC genomics 11. (2010 Aug 4): 459.

    Genetics
    Goldberg ML
    Genetics 191.4 (2012 Aug): 1181-97.

    Journal of cell science
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    Journal of cell science
    Kadrmas JL
    Journal of cell science 125.Pt 13 (2012 Jul 1): 3185-94.

    DNA repair
    Fisher PA
    DNA repair 4.9 (2005 Aug 15): 971-82.

    Journal of structural biology
    Fisher PA
    Journal of structural biology 117.1 (1996 Jul-Aug): 1-15.

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