ADL67.10

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$40.00
SKU: ADL67.10
View product citations for antibody ADL67.10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila, Mosquito, Spodoptera frugiperda
Depositors Notes: Reacts with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL67; alphaL528-622
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 548-620 [Depositor Notes] or aa 528-622 [PMID 8776884].
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:
Antibody Registry ID: AB_528336 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL67.10 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL67.10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.
    Schmitt B
    The Journal of cell biology 137.5 (1997 Jun 2): 1001-16.

    Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation.
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Characterization of the Drosophila melanogaster genome at the nuclear lamina.
    van Steensel B
    Nature genetics 38.9 (2006 Sep): 1005-14.

    Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection.
    Ji S
    Viruses 8.5 (2016 May 7): .

    The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.
    Rzepecki R
    PloS one 7.2 (2012): e32649.

    Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene.
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster.
    Prantera G
    PloS one 11.3 (2016): e0151231.

    Cytoskeletal control of nuclear morphology and chromatin organization.
    Shivashankar GV
    Journal of molecular biology 427.3 (2015 Feb 13): 695-706.

    Genome mapping and characterization of the Anopheles gambiae heterochromatin.
    Sharakhov IV
    BMC genomics 11. (2010 Aug 4): 459.

    Bypassing the Greatwall-Endosulfine pathway: plasticity of a pivotal cell-cycle regulatory module in Drosophila melanogaster and Caenorhabditis elegans.
    Goldberg ML
    Genetics 191.4 (2012 Aug): 1181-97.

    WB References
    IHC References
    IP References
    Epitope Map References
    All References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration.
    Fanto M
    Current biology : CB 27.23 (2017 Dec 4): 3626-3642.e6.

    Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.
    Schmitt B
    The Journal of cell biology 137.5 (1997 Jun 2): 1001-16.

    Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation.
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    Characterization of the Drosophila melanogaster genome at the nuclear lamina.
    van Steensel B
    Nature genetics 38.9 (2006 Sep): 1005-14.

    Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection.
    Ji S
    Viruses 8.5 (2016 May 7): .

    The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.
    Rzepecki R
    PloS one 7.2 (2012): e32649.

    Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene.
    Wallrath LL
    Genetics 171.1 (2005 Sep): 185-96.

    Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster.
    Prantera G
    PloS one 11.3 (2016): e0151231.

    Cytoskeletal control of nuclear morphology and chromatin organization.
    Shivashankar GV
    Journal of molecular biology 427.3 (2015 Feb 13): 695-706.

    Genome mapping and characterization of the Anopheles gambiae heterochromatin.
    Sharakhov IV
    BMC genomics 11. (2010 Aug 4): 459.

    Bypassing the Greatwall-Endosulfine pathway: plasticity of a pivotal cell-cycle regulatory module in Drosophila melanogaster and Caenorhabditis elegans.
    Goldberg ML
    Genetics 191.4 (2012 Aug): 1181-97.

    Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
    Fisher PA
    Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.

    A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted.
    Kadrmas JL
    Journal of cell science 125.Pt 13 (2012 Jul 1): 3185-94.

    The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos.
    Fisher PA
    DNA repair 4.9 (2005 Aug 15): 971-82.

    Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding.
    Fisher PA
    Journal of structural biology 117.1 (1996 Jul-Aug): 1-15.

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