ADL101

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$40.00
SKU: ADL101
View product citations for antibody ADL101 on CiteAb

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Available: 183

DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila, Spodoptera frugiperda
Depositors Notes: Reacts with Sf9 lamin. Reacts with all Dm isoforms; reacts with lamin C. Epitope between aa 261-278 (coil 1b/2).
Antigen Molecular Weight: Predicted: 71 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL67; alphaL528-622
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 261-278 [Depositor Notes]
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:
Antibody Registry ID: AB_528332 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL101 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product ADL101)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

    Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation.
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    The axon guidance gene lola is required for programmed cell death in the Drosophila ovary.
    McCall K
    Developmental biology 304.2 (2007 Apr 15): 771-85.

    Citron kinase is an essential effector of the Pbl-activated Rho signalling pathway in Drosophila melanogaster.
    Saint R
    Development (Cambridge, England) 131.20 (2004 Oct): 5053-63.

    Accumulation of insoluble forms of FUS protein correlates with toxicity in Drosophila.
    Lecourtois M
    Neurobiology of aging 33.5 (2012 May): 1008.e1-15.

    Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope.
    Budnik V
    Cell reports 3.4 (2013 Apr 25): 988-95.

    The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth.
    Pereira PS
    Development (Cambridge, England) 138.2 (2011 Jan): 349-57.

    A global change in RNA polymerase II pausing during the Drosophila midblastula transition.
    Zeitlinger J
    eLife 2. (2013 Aug 13): e00861.

    Heterochromatic breaks move to the nuclear periphery to continue recombinational repair.
    Chiolo I
    Nature cell biology 17.11 (2015 Nov): 1401-11.

    Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene.
    Rohrmann GF
    Virology 364.2 (2007 Aug 1): 475-85.

    dFMRP and Caprin, translational regulators of synaptic plasticity, control the cell cycle at the Drosophila mid-blastula transition.
    Sisson JC
    Development (Cambridge, England) 137.24 (2010 Dec): 4201-9.

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.
    Scholey JM
    The Journal of cell biology 188.1 (2010 Jan 11): 49-68.

    Importin-beta11 regulates synaptic phosphorylated mothers against decapentaplegic, and thereby influences synaptic development and function at the Drosophila neuromuscular junction.
    Schwarz TL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.15 (2010 Apr 14): 5253-68.

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