ADL101
Catalog Fields
Antigen: lamin Dm0
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Fisher, P.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Spodoptera frugiperda
Depositors Institution: SUNY at Stony Brook, Pharmacological Sciences
Antigen Molecular Weight: Predicted: 71 kDa
Depositors Notes: Reacts with Sf9 lamin. Reacts with all Dm isoforms; reacts with lamin C. Epitope between aa 261-278 (coil 1b/2).
Immunogen: Recombinant total protein
Gene: Lam
Alternate Antibody Name: ADL67; alphaL528-622
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS0
Epitope Mapped: Yes
Uniprot ID: P08928
Epitope Location or Sequence: aa 261-278 [Depositor Notes]
Entrez Gene ID: 33782
Immunogen Sequence: Total protein
Antibody Registry ID: AB_528332
Recommended Applications: Immunofluorescence, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL101 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product ADL101)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
Weber K
Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.