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DSHB Data Sheet

Catalog Fields

Product Name/ID: ADL101
Available to For-Profits: Yes
Alternate Antibody Name: ADL67; alphaL528-622
Gene Name: Lam
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528332 
Uniprot ID: P08928 
Entrez Gene ID: 33782 
Clonality: Monoclonal
Immunogen: Recombinant total protein
Immunogen Sequence: Total protein
Myeloma Strain: NS0
Epitope Mapped: Yes
Antigen Name: lamin Dm0
Epitope Location or Sequence: aa 261-278 [Depositor Notes]
Alternate Antigen Name:
Deposit Date: 3/11/2003
Antigen Molecular Weight: Predicted: 71 kDa
Depositor: Fisher, P.A.
Antigen Sequence:
Depositor Institution: SUNY at Stony Brook, Pharmacological Sciences
Antigen Species: Drosophila
Depositor Notes: Reacts with Sf9 lamin. Reacts with all Dm isoforms; reacts with lamin C. Epitope between aa 261-278 (coil 1b/2).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Spodoptera frugiperda
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL101 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product ADL101)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

    Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation.
    Furukawa K
    Developmental biology 284.1 (2005 Aug 1): 219-32.

    The axon guidance gene lola is required for programmed cell death in the Drosophila ovary.
    McCall K
    Developmental biology 304.2 (2007 Apr 15): 771-85.

    Citron kinase is an essential effector of the Pbl-activated Rho signalling pathway in Drosophila melanogaster.
    Saint R
    Development (Cambridge, England) 131.20 (2004 Oct): 5053-63.

    Accumulation of insoluble forms of FUS protein correlates with toxicity in Drosophila.
    Lecourtois M
    Neurobiology of aging 33.5 (2012 May): 1008.e1-15.

    Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope.
    Budnik V
    Cell reports 3.4 (2013 Apr 25): 988-95.

    The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth.
    Pereira PS
    Development (Cambridge, England) 138.2 (2011 Jan): 349-57.

    A global change in RNA polymerase II pausing during the Drosophila midblastula transition.
    Zeitlinger J
    eLife 2. (2013 Aug 13): e00861.

    Heterochromatic breaks move to the nuclear periphery to continue recombinational repair.
    Chiolo I
    Nature cell biology 17.11 (2015 Nov): 1401-11.

    Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene.
    Rohrmann GF
    Virology 364.2 (2007 Aug 1): 475-85.

    dFMRP and Caprin, translational regulators of synaptic plasticity, control the cell cycle at the Drosophila mid-blastula transition.
    Sisson JC
    Development (Cambridge, England) 137.24 (2010 Dec): 4201-9.

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.
    Scholey JM
    The Journal of cell biology 188.1 (2010 Jan 11): 49-68.

    Importin-beta11 regulates synaptic phosphorylated mothers against decapentaplegic, and thereby influences synaptic development and function at the Drosophila neuromuscular junction.
    Schwarz TL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.15 (2010 Apr 14): 5253-68.

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