CD133 HB#7
Catalog Fields
Antigen: CD133/ Prominin-1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R.
Isotype: MIgG1
Depositors Institution: University of Minnesota
Host Species: mouse
Depositors Notes: This antibody recognizes both glycosylated and non-glycoslyated extracellular domains of CD133.
Positive Tested Species Reactivity: Human
Gene: PROM1
Antigen Molecular Weight: predicted molecular weight 97 kDa
Alternate Gene Names: AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Immunogen: Recombinant chimeric protein
Clonality: Monoclonal
Alternate Antibody Name: HC7
Epitope Mapped: No
Myeloma Strain: SP 2/0
Immunogen Sequence: aa180-380 and aa612-765
Uniprot ID: O43490
Additional Information: Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa. This antibody recognizes CD133 is aldehyde fixed cells and tissues (see 25448068 ). RRID:AB_2722053
Entrez Gene ID: 8842
Antibody Registry ID: AB_2722053
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CD133 HB#7 was deposited to the DSHB by Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R. (DSHB Hybridoma Product CD133 HB#7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Identification of a novel monoclonal antibody recognizing CD133.
Ohlfest JR
Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.