Catalog Fields
Antigen: CD133/ Prominin-1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Minnesota
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes both glycosylated and non-glycoslyated extracellular domains of CD133.
Predicted Species Reactivity:
Gene: PROM1
Immunogen: Recombinant chimeric protein
Alternate Gene Names: AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Alternate Antibody Name: HC7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: O43490
Immunogen Sequence: aa180-380 and aa612-765
Entrez Gene ID: 8842
Antibody Registry ID: AB_2722053
Additional Information: Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa. This antibody recognizes CD133 is aldehyde fixed cells and tissues (see 25448068 ). RRID:AB_2722053
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CD133 HB#7 was deposited to the DSHB by Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R. (DSHB Hybridoma Product CD133 HB#7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Identification of a novel monoclonal antibody recognizing CD133.
Ohlfest JR
Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.