CD133 HB#7

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$40.00
SKU: CD133 HB#7
View product citations for antibody CD133 HB#7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD133/ Prominin-1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Minnesota
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes both glycosylated and non-glycoslyated extracellular domains of CD133.
Antigen Molecular Weight: predicted molecular weight 97 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PROM1
Immunogen: Recombinant chimeric protein
Alternate Gene Names: AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Alternate Antibody Name: HC7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: O43490 
Immunogen Sequence: aa180-380 and aa612-765
Entrez Gene ID: 8842 
Additional Characterization:  
Antibody Registry ID: AB_2722053 
Additional Information: Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa. This antibody recognizes CD133 is aldehyde fixed cells and tissues (see 25448068 ). RRID:AB_2722053
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CD133 HB#7 was deposited to the DSHB by Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R. (DSHB Hybridoma Product CD133 HB#7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication

    Identification of a novel monoclonal antibody recognizing CD133.
    Ohlfest JR
    Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.

    IF References
    WB References

    Identification of a novel monoclonal antibody recognizing CD133.
    Ohlfest JR
    Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.

    A Novel Notch-YAP Circuit Drives Stemness and Tumorigenesis in Embryonal Rhabdomyosarcoma.
    Linardic CM
    Molecular cancer research : MCR 15.12 (2017 Dec): 1777-1791.

    IHC References

    Identification of a novel monoclonal antibody recognizing CD133.
    Ohlfest JR
    Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.

    FFPE References

    Identification of a novel monoclonal antibody recognizing CD133.
    Ohlfest JR
    Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.

    FACS References
    All References

    Identification of a novel monoclonal antibody recognizing CD133.
    Ohlfest JR
    Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5.

    Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages.
    Giebel B
    Stem cell reports 3.6 (2014 Dec 9): 1058-72.

    A Novel Notch-YAP Circuit Drives Stemness and Tumorigenesis in Embryonal Rhabdomyosarcoma.
    Linardic CM
    Molecular cancer research : MCR 15.12 (2017 Dec): 1777-1791.

    Targeting CD133 in an in vivo ovarian cancer model reduces ovarian cancer progression.
    Vallera DA
    Gynecologic oncology 130.3 (2013 Sep): 579-87.

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