DAO5

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SKU: DAO5

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DSHB Data Sheet

Catalog Fields

Product Name/ID: DAO5
Available to For-Profits: Yes
Alternate Antibody Name: 5E9
Gene Name: dao-5
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_10573805 
Uniprot ID: Q9XVS4 Q564W7 
RRID:  
Entrez Gene ID: 266845 
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged DAO-5 (C-terminal 221 aa) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: DAO-5
Epitope Location or Sequence: C-terminal 221 aa
Alternate Antigen Name:
Deposit Date: 2/16/2010
Antigen Molecular Weight: Predicted: 73 and 101 kDa; multiple bands from 10 to 150 kDa.
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Antigen Sequence:
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Works on C. elegans whole mounts. Stains the nucleosomes.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: DAO-5 is a homolog of the nucleolar phosphoprotein Nopp140. This protein has been reported to give multiple bands on western due to post-translational modification, proteolysis, dimerization, trimerization and alternative splicing.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DAO5 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product DAO5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ChIP References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Nucleologenesis in the Caenorhabditis elegans embryo.
    Lanctôt C
    PloS one 7.7 (2012): e40290.

    Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.
    Lo SJ
    Cell death & disease 5.4 (2014 Apr 10): e1158.

    Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
    Kimble J
    Proceedings of the National Academy of Sciences of the United States of America 111.10 (2014 Mar 11): 3739-44.

    DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans.
    Labbé JC
    Development (Cambridge, England) 142.24 (2015 Dec 15): 4230-41.

    WB References
    IHC References

    Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.
    Fernandez AG
    microPublication biology 2021. (2021): .

    ChIP References
    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.
    Fernandez AG
    microPublication biology 2021. (2021): .

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Nucleologenesis in the Caenorhabditis elegans embryo.
    Lanctôt C
    PloS one 7.7 (2012): e40290.

    Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.
    Lo SJ
    Cell death & disease 5.4 (2014 Apr 10): e1158.

    Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
    Kimble J
    Proceedings of the National Academy of Sciences of the United States of America 111.10 (2014 Mar 11): 3739-44.

    DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans.
    Labbé JC
    Development (Cambridge, England) 142.24 (2015 Dec 15): 4230-41.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

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