DAO5

(0) No Reviews yet
$40.00
SKU: DAO5
View product citations for antibody DAO5 on CiteAb

In Stock

Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: DAO-5
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains the nucleosomes.
Antigen Molecular Weight: Predicted: 73 and 101 kDa; multiple bands from 10 to 150 kDa.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: dao-5
Immunogen: Recombinant His6-tagged DAO-5 (C-terminal 221 aa) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5E9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal 221 aa
Uniprot ID: Q9XVS4 Q564W7 
Immunogen Sequence: Partial protein
Entrez Gene ID: 266845 
Additional Characterization:
Antibody Registry ID: AB_10573805 
Additional Information: DAO-5 is a homolog of the nucleolar phosphoprotein Nopp140. This protein has been reported to give multiple bands on western due to post-translational modification, proteolysis, dimerization, trimerization and alternative splicing.
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DAO5 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product DAO5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Nucleologenesis in the Caenorhabditis elegans embryo.
    Lanctôt C
    PloS one 7.7 (2012): e40290.

    Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.
    Lo SJ
    Cell death and disease 5. (2014 Apr 10): e1158.

    Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
    Kimble J
    Proceedings of the National Academy of Sciences of the United States of America 111.10 (2014 Mar 11): 3739-44.

    DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans.
    Labbé JC
    Development (Cambridge, England) 142.24 (2015 Dec 15): 4230-41.

    WB References
    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Nucleologenesis in the Caenorhabditis elegans embryo.
    Lanctôt C
    PloS one 7.7 (2012): e40290.

    Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.
    Lo SJ
    Cell death and disease 5. (2014 Apr 10): e1158.

    Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
    Kimble J
    Proceedings of the National Academy of Sciences of the United States of America 111.10 (2014 Mar 11): 3739-44.

    DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans.
    Labbé JC
    Development (Cambridge, England) 142.24 (2015 Dec 15): 4230-41.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review