LMP1
Catalog Fields
Antigen: LMP-1
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: 24 kDa; Apparent: 37 kDa
Depositors Notes: Works on C. elegans whole mounts. Stains lysosomes. Specific by western blot: the 37 kDa band observed in wild type is absent from the lmp-1 mutant (nr2045). On whole mount immunostaining, most of the wild type staining is lost in the lmp-1 mutant (nr2045) except that the immunostaining of the nervous system remained in the mutant and became more pronounced, suggesting some cross reaction with another LAMP protein (see initial publication).
Immunogen: Recombinant His6-tagged LMP-1 (N-terminal aa 1-143 of mature LMP-1) fusion protein
Gene: LMP-1
Alternate Antibody Name: 2E2
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: Q11117
Epitope Location or Sequence: N-terminal aa 1-143 of mature LMP-1
Entrez Gene ID: 180912
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2161795
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: The LAMP family protein lmp-1 is a homolog of the lysosome associated membrane protein family (LAMPs) and is highly expressed and localized to lysosomal-like granules in the C. elegans intestine which can be detected in wild type animals.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LMP1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product LMP1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
4 References
Initial Publication
A monoclonal antibody toolkit for C. elegans.
Nonet ML
PloS one 5.4 (2010 Apr 13): e10161.