HSP60

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$45.00 to $425.00
SKU: HSP60
View product citations for antibody HSP60 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: HSP-60
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. briggsae, C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: 60 kDa; Apparent: 65 kDa
Depositors Notes: Works on C. elegans whole mounts
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant His6-tagged HSP-60 (aa 1-547) fusion protein
Gene: hsp-60
Alternate Antibody Name: 1H12
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: No
Uniprot ID: P50140 
Epitope Location or Sequence:
Entrez Gene ID: 175316 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10572252 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: The Chaperonin homolog Hsp-60, mitochondrial, is a mitochondrial targeted heat shock protein useful as a marker of mitochondria
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HSP60 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product HSP60)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Regulation of CED-3 caspase localization and activation by C. elegans nuclear-membrane protein NPP-14.
    Xue D
    Nature structural & molecular biology 23.11 (2016 Nov): 958-964.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response.
    Dillin A
    Cell 166.6 (2016 Sep 8): 1539-1552.e16.

    IHC References
    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    Autophagy facilitates mitochondrial rebuilding after acute heat stress via a DRP-1-dependent process.
    Legouis R
    The Journal of cell biology 220.4 (2021 Apr 5): .

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    Regulation of CED-3 caspase localization and activation by C. elegans nuclear-membrane protein NPP-14.
    Xue D
    Nature structural & molecular biology 23.11 (2016 Nov): 958-964.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response.
    Dillin A
    Cell 166.6 (2016 Sep 8): 1539-1552.e16.

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