H-69

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$40.00
SKU: H-69
View product citations for antibody H-69 on CiteAb

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Available: 45

DSHB Data Sheet

Catalog Fields

Antigen: endoplasmic reticulum, rough, glycoprotein
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes: IHC notes: localized in RER, induced in F9 cells
Antigen Molecular Weight: 76 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: NIH/3T3 mouse embryo fibroblast tissue culture cell membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-NSI/1-Ag4-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2174424 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H-69 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product H-69)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    All References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

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