1D4B

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$40.00
SKU: 1D4B
View product citations for antibody 1D4B on CiteAb

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DSHB Data Sheet

Details

CD107a

Catalog Fields

Antigen: LAMP-1
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Pharmacology & Molecular Sciences, Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes:
Antigen Molecular Weight: Predicted: 41 kDa; Apparent: 110kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lamp1
Immunogen: NIH/3T3 mouse embryo fibroblast tissue culture cell membranes
Alternate Gene Names: AI196048, CD107a, Lamp-1
Alternate Antibody Name: CD107a
Clonality: Monoclonal
Alternate Antigen Name: Lysosomal membrane glycoprotein A, LGP-120, CD107a
Epitope Mapped: Yes
Myeloma Strain: P3-NSI/1AG4-1
Epitope Location or Sequence: Lumenal region
Uniprot ID: P11438 
Immunogen Sequence:
Entrez Gene ID: 16783 
Additional Characterization:
Antibody Registry ID: AB_528127 
Additional Information: RRID: AB_528127
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D4B was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product 1D4B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

28 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    IF References

    Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.
    Griffiths G
    The EMBO journal 19.2 (2000 Jan 17): 199-212.

    SseF and SseG are translocated effectors of the type III secretion system of Salmonella pathogenicity island 2 that modulate aggregation of endosomal compartments.
    Hensel M
    Cellular microbiology 4.12 (2002 Dec): 813-24.

    Early intraneuronal Abeta deposition in the hippocampus of APP transgenic mice.
    Jin LW
    Neuroreport 14.1 (2003 Jan 20): 123-9.

    Dynamics of intracellular bacterial replication at the single cell level.
    Holden DW
    Proceedings of the National Academy of Sciences of the United States of America 107.8 (2010 Feb 23): 3746-51.

    Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration.
    Strickland S
    Neurobiology of aging 36.2 (2015 Feb): 608-17.

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular and molecular biology research 41.5 (1995): 405-20.

    Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons.
    Sheng ZH
    The Journal of cell biology 217.9 (2018 Sep 3): 3127-3139.

    Impaired Lysosomal Integral Membrane Protein 2-dependent Peroxiredoxin 6 Delivery to Lamellar Bodies Accounts for Altered Alveolar Phospholipid Content in Adaptor Protein-3-deficient pearl Mice.
    Guttentag S
    The Journal of biological chemistry 291.16 (2016 Apr 15): 8414-27.

    Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
    Dikic I
    eLife 6. (2017 Jun 15): .

    Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis.
    Bovolenta P
    Nature neuroscience 22.8 (2019 Aug): 1258-1268.

    Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts.
    Sheng ZH
    Nature communications 10.1 (2019 Aug 13): 3645.

    WB References
    IHC References

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.
    Ganley IG
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 621-642.

    IP References
    All References

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.
    Ganley IG
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 621-642.

    Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.
    Griffiths G
    The EMBO journal 19.2 (2000 Jan 17): 199-212.

    SseF and SseG are translocated effectors of the type III secretion system of Salmonella pathogenicity island 2 that modulate aggregation of endosomal compartments.
    Hensel M
    Cellular microbiology 4.12 (2002 Dec): 813-24.

    Early intraneuronal Abeta deposition in the hippocampus of APP transgenic mice.
    Jin LW
    Neuroreport 14.1 (2003 Jan 20): 123-9.

    Dynamics of intracellular bacterial replication at the single cell level.
    Holden DW
    Proceedings of the National Academy of Sciences of the United States of America 107.8 (2010 Feb 23): 3746-51.

    Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration.
    Strickland S
    Neurobiology of aging 36.2 (2015 Feb): 608-17.

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular and molecular biology research 41.5 (1995): 405-20.

    Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons.
    Sheng ZH
    The Journal of cell biology 217.9 (2018 Sep 3): 3127-3139.

    Impaired Lysosomal Integral Membrane Protein 2-dependent Peroxiredoxin 6 Delivery to Lamellar Bodies Accounts for Altered Alveolar Phospholipid Content in Adaptor Protein-3-deficient pearl Mice.
    Guttentag S
    The Journal of biological chemistry 291.16 (2016 Apr 15): 8414-27.

    Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
    Dikic I
    eLife 6. (2017 Jun 15): .

    Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis.
    Bovolenta P
    Nature neuroscience 22.8 (2019 Aug): 1258-1268.

    Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts.
    Sheng ZH
    Nature communications 10.1 (2019 Aug 13): 3645.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.
    Haas A
    Molecular biology of the cell 29.4 (2018 Feb 15): 452-465.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins.
    Mellman I
    Proceedings of the National Academy of Sciences of the United States of America 85.20 (1988 Oct): 7577-81.

    Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells.
    August JT
    The Journal of biological chemistry 256.13 (1981 Jul 10): 7023-7.

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Lysosomal cathepsin B participates in the podosome-mediated extracellular matrix degradation and invasion via secreted lysosomes in v-Src fibroblasts.
    Band H
    Cancer research 68.22 (2008 Nov 15): 9147-56.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the localization of active Src at focal adhesions.
    Band H
    Proceedings of the National Academy of Sciences of the United States of America 107.37 (2010 Sep 14): 16107-12.

    alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state.
    Brentani RR
    Cancer research 56.7 (1996 Apr 1): 1682-9.

    A kinetic analysis of biosynthesis and localization of a lysosome-associated membrane glycoprotein.
    August JT
    Archives of biochemistry and biophysics 249.2 (1986 Sep): 522-32.

    Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2.
    August JT
    Biochemical Society symposium 51. (1986): 97-112.

    Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells.
    August JT
    Archives of biochemistry and biophysics 239.2 (1985 Jun): 574-86.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Murine cell surface glycoproteins. Identification, purification, and characterization of a major glycosylated component of 110,000 daltons by use of a monoclonal antibody.
    August JT
    The Journal of biological chemistry 257.7 (1982 Apr 10): 3970-7.

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